{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T18:23:14Z","timestamp":1760898194434,"version":"3.37.3"},"reference-count":30,"publisher":"European Respiratory Society (ERS)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur Respir J"],"accepted":{"date-parts":[[2013,1,3]]},"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:p>The proprotein convertases (PCs) are serine proteases responsible for the proteolytic maturation of many precursor proteins involved in upper airway remodelling during nasal polyposis. We have previously found that PC1\/3 is expressed in human nasal mucosa.<\/jats:p><jats:p>However, whether PC1\/3 is related to nasal polyp formation has not been investigated. To gain insight into the functional role of PC1\/3 in nasal polyps, we determined PC1\/3 expression in nasal polyps by immunostaining, Western blotting and enzyme assays and generated stable cells expressing PC1\/3 using airway epithelial cell line NCI-H292.<\/jats:p><jats:p>Nasal polyps exhibit increased PC1\/3 expression compared to normal nasal mucosa. PC1\/3 was expressed in neuroendocrine cells in normal nasal mucosa and it was also expressed in goblet and ciliated cells in nasal polyps. NCI-H292 cells stably expressing PC1\/3 displayed morphological changes, enhanced cell proliferation and migration, downregulation of E-cadherin and cytokeratins and upregulation of N-cadherin, vimentin, matrix metalloproteinase-2, collagen-I, snail and twist. Importantly, PC1\/3 expression was positively correlated with epithelial-mesenchymal transition in cultured human nasal epithelial cells and in nasal polyps.<\/jats:p><jats:p>Taken together, our data suggest that PC1\/3 overexpression induces morphological and phenotypic epithelial-mesenchymal transition changes of airway epithelial cells and these changes may contribute to the pathogenesis of nasal polyps.<\/jats:p>","DOI":"10.1183\/09031936.00100412","type":"journal-article","created":{"date-parts":[[2013,1,12]],"date-time":"2013-01-12T05:41:15Z","timestamp":1357969275000},"page":"1379-1390","source":"Crossref","is-referenced-by-count":11,"title":["Overexpressed proprotein convertase 1\/3 induces an epithelial-mesenchymal transition in airway 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