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In the present study, we used volumetric computed tomography (VCT) as a new method for longitudinal<jats:italic>in vivo<\/jats:italic>monitoring of early lesions and disease progression in CF-like lung disease in \u03b2-epithelial Na<jats:sup>+<\/jats:sup>channel (ENaC)-transgenic (TG) mice.<\/jats:p><jats:p>Using a VCT scanner prototype (80 kV, 50 mA\u00b7s, scan time 19 s and spatial resolution 200 \u03bcm), \u03b2ENaC-TG mice and wild-type (WT) littermates were examined longitudinally at 10 time-points from neonatal to adult ages, and VCT images were assessed by qualitative and quantitative morphological parameters.<\/jats:p><jats:p>We demonstrate that VCT detected early-onset airway mucus obstruction, diffuse infiltrates, atelectasis and air trapping as characteristic abnormalities in \u03b2ENaC-TG mice. Furthermore, we show that early tracheal mucus obstruction predicted mortality in \u03b2ENaC-TG mice and that the density of lung parenchyma was significantly reduced at all time-points in \u03b2ENaC-TG compared with WT mice (median\u00b1<jats:sc>sem<\/jats:sc>-558\u00b18 HU in WT<jats:italic>versus<\/jats:italic>-686\u00b116 HU in \u03b2ENaC-TG at 6 weeks of age; p&lt;0.005).<\/jats:p><jats:p>Our study demonstrates that VCT is a sensitive, noninvasive technique for early detection and longitudinal monitoring of morphological abnormalities of CF-like lung disease in mice, and may thus provide a useful tool for pre-clinical<jats:italic>in vivo<\/jats:italic>evaluation of novel treatment strategies for CF.<\/jats:p>","DOI":"10.1183\/09031936.00149810","type":"journal-article","created":{"date-parts":[[2011,4,9]],"date-time":"2011-04-09T01:19:03Z","timestamp":1302311943000},"page":"1060-1070","source":"Crossref","is-referenced-by-count":47,"title":["<i>In vivo<\/i>monitoring of cystic fibrosis-like lung disease in mice by volumetric computed tomography"],"prefix":"10.1183","volume":"38","author":[{"given":"M.O.","family":"Wielp\u00fctz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Eichinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Leotta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Hirtz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.H.","family":"Bartling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Semmler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H-U.","family":"Kauczor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Puderbach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.A.","family":"Mall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"81","published-online":{"date-parts":[[2011,4,8]]},"reference":[{"key":"2024102020375017000_38.5.1060.1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2570460"},{"key":"2024102020375017000_38.5.1060.2","unstructured":"Welsh MJ Ramsey BW Accurso F . 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