{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T20:24:38Z","timestamp":1770495878196,"version":"3.49.0"},"reference-count":50,"publisher":"European Respiratory Society (ERS)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur Respir J"],"accepted":{"date-parts":[[2008,3,18]]},"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:p>Airway epithelial cells are exposed to environmental toxicants that result in airway injury. Naphthalene (NA) causes site-selective damage to Clara cells in mouse distal airways. N-terminally truncated recombinant human keratinocyte growth factor (\u0394N23-KGF) protects against acute lung injury. The present study investigated whether or not \u0394N23-KGF protects against NA-induced acute Clara cell damage by measuring airway responses specifically and in order to identify underlying molecular mechanisms.<\/jats:p><jats:p>Mice were treated with \u0394N23-KGF or PBS 33\u2005h prior to injection of 200\u2005mg\u00b7kg body weight<jats:sup>\u22121<\/jats:sup>NA. Lung function was analysed by head-out body plethysmography. Distal airways isolated by microdissection were assessed for cell permeability using ethidium homodimer-1. Immunohistochemistry of Clara cell-specific protein in conjunction with a physical dissector was used to quantify Clara cell numbers. RNA was isolated from frozen airways in order to analyse gene expression using quantitative RT-PCR.<\/jats:p><jats:p>\u0394N23-KGF prevented NA-induced airflow limitation and Clara cell permeability, and resulted in twice as many Clara cells compared with PBS pre-treatment. \u0394N23-KGF-pre-treated mice exhibited increased expression of proliferating cell nuclear antigen mRNA. Cytochrome P<jats:sub>450<\/jats:sub>isoform 2F2, which converts NA into its toxic metabolite, was reduced by \u223c50%.<\/jats:p><jats:p>The present results demonstrate that pre-treatment with N-terminally truncated recombinant human keratinocyte growth factor protects against naphthalene-induced injury. This suggests that N-terminally truncated recombinant human keratinocyte growth factor exerts its beneficial effect through a decrease in the expression of cytochrome P<jats:sub>450<\/jats:sub>isoform 2F2.<\/jats:p>","DOI":"10.1183\/09031936.00155107","type":"journal-article","created":{"date-parts":[[2008,4,3]],"date-time":"2008-04-03T00:26:58Z","timestamp":1207182418000},"page":"694-704","source":"Crossref","is-referenced-by-count":30,"title":["Keratinocyte growth factor protects against Clara cell injury induced by naphthalene"],"prefix":"10.1183","volume":"32","author":[{"given":"A. \u00d6.","family":"Yildirim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Veith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Rausch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Kilb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. S.","family":"Van Winkle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Fehrenbach","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"81","published-online":{"date-parts":[[2008,4,2]]},"reference":[{"key":"2024102019344799000_32.3.694.1","doi-asserted-by":"publisher","DOI":"10.1513\/pats.200605-126SF"},{"key":"2024102019344799000_32.3.694.2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa040610"},{"key":"2024102019344799000_32.3.694.3","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.02110s4505"},{"key":"2024102019344799000_32.3.694.4","unstructured":"Plopper CG, Hyde DM. Epithelial cells of bronchioles. In: Parent RA., ed. Comparative Biology of the Normal Lung. Boca Raton, CRC Press, 1992; pp.\u200585\u201392"},{"key":"2024102019344799000_32.3.694.5","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.24.3.4247"},{"key":"2024102019344799000_32.3.694.6","doi-asserted-by":"publisher","DOI":"10.1007\/s00420-003-0458-1"},{"key":"2024102019344799000_32.3.694.7","doi-asserted-by":"publisher","DOI":"10.1007\/s00420-004-0593-3"},{"key":"2024102019344799000_32.3.694.8","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/18.11.2035"},{"key":"2024102019344799000_32.3.694.9","first-page":"800","volume":"269","year":"1995","journal-title":"Am J Physiol"},{"key":"2024102019344799000_32.3.694.10","first-page":"L101","volume":"266","year":"1994","journal-title":"Am J Physiol"},{"key":"2024102019344799000_32.3.694.11","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1165\/ajrcmb.17.3.2774","volume":"17","year":"1997","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2024102019344799000_32.3.694.12","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0022-3565(24)34896-7","volume":"290","year":"1999","journal-title":"J Pharmacol Exp Ther"},{"key":"2024102019344799000_32.3.694.13","first-page":"553","volume":"67","year":"1992","journal-title":"Lab Invest"},{"key":"2024102019344799000_32.3.694.14","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1152\/ajplung.00439.2001","volume":"282","year":"2002","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2024102019344799000_32.3.694.15","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002030210"},{"key":"2024102019344799000_32.3.694.16","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.10451"},{"key":"2024102019344799000_32.3.694.17","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.02.00022702"},{"key":"2024102019344799000_32.3.694.18","doi-asserted-by":"crossref","first-page":"195","DOI":"10.2307\/3579855","volume":"150","year":"1998","journal-title":"Radiat Res"},{"key":"2024102019344799000_32.3.694.19","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.10115"},{"key":"2024102019344799000_32.3.694.20","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00011606"},{"key":"2024102019344799000_32.3.694.21","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199912)181:3<424::AID-JCP6>3.0.CO;2-Z"},{"key":"2024102019344799000_32.3.694.22","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2006-0384OC"},{"key":"2024102019344799000_32.3.694.23","doi-asserted-by":"publisher","DOI":"10.1006\/geno.2001.6610"},{"key":"2024102019344799000_32.3.694.24","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.43.100901.140251"},{"key":"2024102019344799000_32.3.694.25","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzj013"},{"key":"2024102019344799000_32.3.694.26","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.14.6.8652187"},{"key":"2024102019344799000_32.3.694.27","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2006.04.006"},{"key":"2024102019344799000_32.3.694.28","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00450.2004"},{"key":"2024102019344799000_32.3.694.29","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00309.2001"},{"key":"2024102019344799000_32.3.694.30","doi-asserted-by":"publisher","DOI":"10.1080\/01926230600713509"},{"key":"2024102019344799000_32.3.694.31","first-page":"8","volume":"38","year":"2008","journal-title":"TNF-\u03b1 from macrophages enhances LPS-induced Clara cell expression of keratinocyte-derived chemokine. Am J Respir Cell Mol Biol"},{"key":"2024102019344799000_32.3.694.32","doi-asserted-by":"publisher","DOI":"10.1016\/j.etp.2004.12.004"},{"key":"2024102019344799000_32.3.694.33","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1111\/j.1749-6632.2000.tb05549.x","volume":"923","year":"2000","journal-title":"Ann NY Acad Sci"},{"key":"2024102019344799000_32.3.694.34","doi-asserted-by":"crossref","first-page":"L1146","DOI":"10.1152\/ajplung.2000.279.6.L1146","volume":"279","year":"2000","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2024102019344799000_32.3.694.35","first-page":"353","volume":"261","year":"1992","journal-title":"J Pharmacol Exp Ther"},{"key":"2024102019344799000_32.3.694.36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1165\/ajrcmb.15.1.8679213","volume":"15","year":"1996","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2024102019344799000_32.3.694.37","doi-asserted-by":"crossref","first-page":"L791","DOI":"10.1152\/ajpcell.1995.269.3.C791","volume":"269","year":"1995","journal-title":"Am J Physiol"},{"key":"2024102019344799000_32.3.694.38","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64727-X"},{"key":"2024102019344799000_32.3.694.39","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64169-7"},{"key":"2024102019344799000_32.3.694.40","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2005-0332OC"},{"key":"2024102019344799000_32.3.694.41","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/S0002-9440(10)64375-1","volume":"160","year":"2002","journal-title":"Am J Pathol"},{"key":"2024102019344799000_32.3.694.42","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0610770104"},{"key":"2024102019344799000_32.3.694.43","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1165\/ajrcmb.22.2.3674","volume":"22","year":"2000","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2024102019344799000_32.3.694.44","doi-asserted-by":"publisher","DOI":"10.1007\/s002040050101"},{"key":"2024102019344799000_32.3.694.45","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1152\/japplphysiol.01121.2001","volume":"93","year":"2002","journal-title":"J Appl Physiol"},{"key":"2024102019344799000_32.3.694.46","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-6-139"},{"key":"2024102019344799000_32.3.694.47","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00783.2003"},{"key":"2024102019344799000_32.3.694.48","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00028.2007"},{"key":"2024102019344799000_32.3.694.49","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.17.2.f138"},{"key":"2024102019344799000_32.3.694.50","first-page":"1256","volume":"278","year":"2000","journal-title":"Am J Physiol Lung Cell Mol Physiol"}],"container-title":["European Respiratory Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1183\/09031936.00155107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T06:10:08Z","timestamp":1740377408000},"score":1,"resource":{"primary":{"URL":"https:\/\/publications.ersnet.org\/lookup\/doi\/10.1183\/09031936.00155107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,4,2]]},"references-count":50,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2008,8,29]]},"published-print":{"date-parts":[[2008,9]]}},"alternative-id":["10.1183\/09031936.00155107"],"URL":"https:\/\/doi.org\/10.1183\/09031936.00155107","relation":{},"ISSN":["0903-1936","1399-3003"],"issn-type":[{"value":"0903-1936","type":"print"},{"value":"1399-3003","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,4,2]]}}}