{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T22:54:41Z","timestamp":1791327281997,"version":"4.3.1"},"reference-count":40,"publisher":"European Respiratory Society (ERS)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur Respir J"],"accepted":{"date-parts":[[2011,1,2]]},"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:p>There is increasing interest in using the cyanogenic properties of<jats:italic>Pseudomonas aeruginosa<\/jats:italic>to develop a nonmicrobiological method for its detection. Prior to this, the variation in cyanide production between different<jats:italic>P. aeruginosa<\/jats:italic>strains needs to be investigated.<\/jats:p><jats:p>Hydrogen cyanide (HCN) released into the gas phase by 96 genotyped<jats:italic>P. aeruginosa<\/jats:italic>samples was measured using selected ion flow tube-mass spectrometry after 24, 48, 72 and 96 h of incubation. The HCN produced by a range of non-<jats:italic>P. aeruginosa<\/jats:italic>cultures and incubated blank plates was also measured.<\/jats:p><jats:p>All<jats:italic>P. aeruginosa<\/jats:italic>strains produced more HCN than the control samples, which generated extremely low levels. Analysis across all time-points demonstrated that nonmucoid samples produced more HCN than the mucoid samples (p=0.003), but this relationship varied according to strain. There were clear differences in the headspace HCN concentration for different strains. Multivariate analysis of headspace HCN for the commonest strains (Liverpool, Midlands_1 and Stoke-on-Trent, UK) revealed a significant effect of strain (p&lt;0.001) and a borderline interaction of strain and phenotype (p=0.051).<\/jats:p><jats:p>This evidence confirms that all<jats:italic>P. aeruginosa<\/jats:italic>strains produce HCN but to varying degrees and generates interest in the possible future clinical applications of the cyanogenic properties of<jats:italic>P. aeruginosa<\/jats:italic>.<\/jats:p>","DOI":"10.1183\/09031936.00166510","type":"journal-article","created":{"date-parts":[[2011,1,29]],"date-time":"2011-01-29T01:58:06Z","timestamp":1296266286000},"page":"409-414","source":"Crossref","is-referenced-by-count":54,"title":["Variation in hydrogen cyanide production between different strains of<i>Pseudomonas 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