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We therefore hypothesised that different PM constituents may contribute through common mechanisms to induce CXCL8.<\/jats:p><jats:p>Human bronchial epithelial cells (BEAS-2B) were exposed to different PM components (Zn<jats:sup>2+<\/jats:sup>\/Fe<jats:sup>2+<\/jats:sup>salts, 1-nitropyrene, lipopolysaccharide and diesel exhaust\/mineral particles). Gene expression patterns were detected by real-time PCR array. CXCL8 responses were measured by real-time PCR and ELISA. CXCL8 regulation was assessed with a broad inhibitor panel and neutralising antibodies. Epidermal growth factor receptor (EGFR) phosphorylation was examined by immunoprecipitation and Western blotting.<\/jats:p><jats:p>Component-induced gene expression was mainly linked to nuclear factor-\u03baB, Ca<jats:sup>2+<\/jats:sup>\/protein kinase C, phospholipase C, low-density lipoprotein and mitogenic signalling. Many inhibitors attenuated CXCL8 release induced by all PM components, but to varying extents. However, EGFR inhibition strongly reduced CXCL8 release induced by all test compounds and selected compounds increased EGFR phosphorylation. Interference with transforming growth factor (TGF)-\u03b1 or tumour necrosis factor-\u03b1-converting enzyme (TACE), which mediates TGF-\u03b1 ectodomain shedding, also attenuated CXCL8 release.<\/jats:p><jats:p>Different PM constituents induced CXCL8 partly through similar signalling pathways but the relative importance of the different pathways varied. However, TACE\/TGF-\u03b1\/EGFR signalling appears to be a convergent pathway regulating innate immune responses of airway epithelial cells upon exposure to multiple airborne pollutants.<\/jats:p>","DOI":"10.1183\/09031936.00171110","type":"journal-article","created":{"date-parts":[[2011,5,4]],"date-time":"2011-05-04T00:46:18Z","timestamp":1304469978000},"page":"1189-1199","source":"Crossref","is-referenced-by-count":39,"title":["TACE\/TGF-\u03b1\/EGFR regulates CXCL8 in bronchial epithelial cells exposed to particulate matter 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