{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T07:01:03Z","timestamp":1790406063476,"version":"4.1.0"},"reference-count":35,"publisher":"European Respiratory Society (ERS)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur Respir J"],"accepted":{"date-parts":[[2010,4,12]]},"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:p>The airway epithelium is a central effector tissue in allergic inflammation and T-helper cell (Th) type 2-driven epithelial responses, such as mucus hypersecretion contribute to airflow obstruction in allergic airway disease. Previous<jats:italic>in vitro<\/jats:italic>studies demonstrated that Th2 cytokines also act as potent modulators of epithelial ion transport and fluid secretion, but the<jats:italic>in vivo<\/jats:italic>effect of allergic inflammation on airway ion transport remains unknown.<\/jats:p><jats:p>We, therefore, induced allergic inflammation by intratracheal instillation of<jats:italic>Aspergillus fumigatus<\/jats:italic>extract or interleukin-13 in mice and determined effects on ion transport in native tracheal and bronchial tissues.<\/jats:p><jats:p>We demonstrate that allergic inflammation enhanced basal Cl<jats:sup>-<\/jats:sup>secretion in both airway regions and inhibited epithelial Na<jats:sup>+<\/jats:sup>channel (ENaC)-mediated Na<jats:sup>+<\/jats:sup>absorption and increased Ca<jats:sup>2+<\/jats:sup>-dependent Cl<jats:sup>-<\/jats:sup>secretion in bronchi. Allergen-induced alterations in bronchial ion transport were associated with reduced transcript levels of \u03b1-, \u03b2- and \u03b3ENaC, and were largely abrogated in signal transducer and activator of transcription (Stat)6<jats:sup>-\/-<\/jats:sup>mice.<\/jats:p><jats:p>Our studies demonstrate that Th2-dependent airway inflammation produced a pro-secretory ion transport phenotype<jats:italic>in vivo<\/jats:italic>, which was largely Stat6-dependent. These results suggest that Th2-mediated fluid secretion may improve airway surface hydration and clearance of mucus that is hypersecreted in allergic airway diseases such as asthma, and identify epithelial Stat6 signalling as a potential therapeutic target to promote mucus hydration and airway clearance.<\/jats:p>","DOI":"10.1183\/09031936.00181209","type":"journal-article","created":{"date-parts":[[2010,4,23]],"date-time":"2010-04-23T00:46:18Z","timestamp":1271983578000},"page":"1436-1447","source":"Crossref","is-referenced-by-count":58,"title":["Allergic airway inflammation induces a pro-secretory epithelial ion transport phenotype in 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