{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:23:52Z","timestamp":1790616232416,"version":"4.1.0"},"reference-count":41,"publisher":"European Respiratory Society (ERS)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur Respir J"],"accepted":{"date-parts":[[2003,3,11]]},"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:p>This study examined inflammatory responses from primary cultured human bronchial epithelial cells in chronic obstructive pulmonary disease (COPD) and the clinical factors modulating them.<\/jats:p><jats:p>Epithelial cells from bronchoscopic biopsies from 14 patients with COPD ((mean\u00b1sd) age 74.6\u00b15.7\u2005yrs, forced expiratory volume in one second (FEV<jats:sub>1<\/jats:sub>) 1.21\u00b10.36\u2005L, FEV<jats:sub>1<\/jats:sub>% predicted 51.1\u00b115.8%, 51.5\u00b124.0 pack-yrs of smoking, inhaled steroid dosage 1237.5\u00b1671.0\u2005\u00b5g\u00b7day<jats:sup>\u22121<\/jats:sup>, Medical Research Council (MRC) dyspnoea score 3.18\u00b11.33) and eight current\/exsmokers with normal pulmonary function (age 60.4\u00b113.5\u2005yrs, FEV<jats:sub>1<\/jats:sub>2.66\u00b11.27\u2005L, FEV<jats:sub>1<\/jats:sub>% pred 89.6\u00b117.7%, 49\u00b144 pack-yrs of smoking, MRC dyspnoea score 1\u00b10) were grown in primary culture and exposed to 50\u2005ng\u00b7mL<jats:sup>\u22121<\/jats:sup>tumour necrosis factor\u2010\u03b1.<\/jats:p><jats:p>Stimulated COPD cells produced significantly more interleukin (IL)\u20106 at 24 and 48\u2005h, and IL\u20108 at 6 and 24\u2005h than unstimulated COPD cells. This response was not seen in cells from current\/exsmokers. IL\u20106 and IL\u20108 production was lower in COPD patients taking inhaled steroids.<\/jats:p><jats:p>Following an inflammatory stimulus, bronchial epithelial cells in chronic obstructive pulmonary disease show a significant cytokine response not seen in smokers with normal pulmonary function and this may be modified by inhaled steroid therapy.<\/jats:p>","DOI":"10.1183\/09031936.03.00093703","type":"journal-article","created":{"date-parts":[[2003,6,27]],"date-time":"2003-06-27T21:21:20Z","timestamp":1056748880000},"page":"94-99","source":"Crossref","is-referenced-by-count":46,"title":["Airway epithelial inflammatory responses and clinical parameters in 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