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The present study aimed to examine the influence of macrolide antibiotics, roxithromycin (RXM) and josamycin (JM) on matrix metalloproteinase (MMP) production from nasal polyp fibroblasts (NPF)<jats:italic>in vitro<\/jats:italic>.<\/jats:p><jats:p>NPF, at a concentration of 2.5\u00d710<jats:sup>5<\/jats:sup>cells\u00b7mL<jats:sup>\u22121<\/jats:sup>, were stimulated with tumour necrosis factor (TNF)-\u03b1 in the presence of various concentrations of RXM or JM for 24\u2005h. MMP\u20102 and \u20109 levels in culture supernatants were analysed by ELISA, and MMP mRNA expression was examined by RT-PCR. The influence of RXM on nuclear factor (NF)-\u03baB and activator protein (AP)\u20101 activation was also examined.<\/jats:p><jats:p>Addition of RXM (but not JM) at 5.0 and 7.5 \u00b5g\u00b7mL<jats:sup>\u22121<\/jats:sup>significantly suppressed the production of MMP\u20102 and \u20109 from NPF induced by TNF-\u03b1 stimulation. RXM also suppressed MMP mRNA expression through the inhibition of NF-\u03baB and AP-1 activation.<\/jats:p><jats:p>The present results suggest that the suppressive activity of roxithromycin on MMP\u20102 and \u20109 production is, in part, responsible for the therapeutic action of macrolides on chronic airway inflammatory diseases.<\/jats:p>","DOI":"10.1183\/09031936.04.00057104","type":"journal-article","created":{"date-parts":[[2004,4,21]],"date-time":"2004-04-21T23:05:33Z","timestamp":1082588733000},"page":"671-678","source":"Crossref","is-referenced-by-count":54,"title":["Suppression of matrix metalloproteinase production from nasal fibroblasts by macrolide antibiotics<i>in vitro<\/i>"],"prefix":"10.1183","volume":"23","author":[{"given":"K.","family":"Kanai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Asano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Hisamitsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Suzaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"81","published-online":{"date-parts":[[2004,5,1]]},"reference":[{"key":"2024101810474212000_23.5.671.1","first-page":"16","volume":"101","year":"1992","journal-title":"Ann Otol Rhinol Laryngol"},{"key":"2024101810474212000_23.5.671.2","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1164\/ajrccm.157.6.9710075","volume":"157","year":"1998","journal-title":"Am J Respir Crit Care Med"},{"key":"2024101810474212000_23.5.671.3","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1007\/BF03256601","volume":"1","year":"2002","journal-title":"Am J Respir Med"},{"key":"2024101810474212000_23.5.671.4","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)78360-4"},{"key":"2024101810474212000_23.5.671.5","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)11081-6"},{"key":"2024101810474212000_23.5.671.6","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(84)90299-9"},{"key":"2024101810474212000_23.5.671.7","first-page":"449","volume":"68","year":"1986","journal-title":"J Invest Dermatol"},{"key":"2024101810474212000_23.5.671.8","doi-asserted-by":"publisher","DOI":"10.1159\/000236773"},{"key":"2024101810474212000_23.5.671.9","doi-asserted-by":"publisher","DOI":"10.1080\/09629350120102343"},{"key":"2024101810474212000_23.5.671.10","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1080\/09629359990351","volume":"8","year":"1999","journal-title":"Med Inflamm"},{"key":"2024101810474212000_23.5.671.11","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-5769(01)00116-3"},{"key":"2024101810474212000_23.5.671.12","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.16.3.9070604"},{"key":"2024101810474212000_23.5.671.13","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-1811(00)00128-6"},{"key":"2024101810474212000_23.5.671.14","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1165\/ajrcmb\/7.2.120","volume":"7","year":"1992","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2024101810474212000_23.5.671.15","doi-asserted-by":"publisher","DOI":"10.1067\/mlc.2001.112726"},{"key":"2024101810474212000_23.5.671.16","doi-asserted-by":"publisher","DOI":"10.1080\/00016480310002519"},{"key":"2024101810474212000_23.5.671.17","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.1917"},{"key":"2024101810474212000_23.5.671.18","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64205-8"},{"key":"2024101810474212000_23.5.671.19","first-page":"164","volume":"36","year":"1988","journal-title":"Chemotherapy"},{"key":"2024101810474212000_23.5.671.20","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S1567-5769(00)00023-0","volume":"1","year":"2001","journal-title":"Int Immunopharmac"},{"key":"2024101810474212000_23.5.671.21","unstructured":"Tomashefski JF, Abramowsky CR, Dahms BB. 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