{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T05:22:53Z","timestamp":1788153773134,"version":"build-2803163510"},"reference-count":38,"publisher":"European Respiratory Society (ERS)","issue":"3","funder":[{"DOI":"10.13039\/501100001186","name":"Australian Cystic Fibrosis Research Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001186","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National PCD Centre at UHS"},{"name":"NHS England"},{"name":"COST Action BEAT-PCD","award":["BM1407"],"award-info":[{"award-number":["BM1407"]}]}],"content-domain":{"domain":["publications.ersnet.org"],"crossmark-restriction":true},"short-container-title":["Eur Respir J"],"accepted":{"date-parts":[[2017,6,4]]},"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:p>Non-typeable<jats:italic>Haemophilus influenzae<\/jats:italic>(NTHi) is the most common pathogen in primary ciliary dyskinesia (PCD) patients. We hypothesised that abnormal ciliary motility and low airway nitric oxide (NO) levels on airway epithelial cells from PCD patients might be permissive for NTHi colonisation and biofilm development.<\/jats:p><jats:p>We used a primary epithelial cell co-culture model to investigate NTHi infection. Primary airway epithelial cells from PCD and non-PCD patients were differentiated to ciliation using an air\u2013liquid interface culture and then co-cultured with NTHi.<\/jats:p><jats:p>NTHi adherence was greater on PCD epithelial cells compared to non-PCD cells (p&lt;0.05) and the distribution of NTHi on PCD epithelium showed more aggregated NTHi in biofilms (p&lt;0.001). Apart from defective ciliary motility, PCD cells did not significantly differ from non-PCD epithelial cells in the degree of ciliation and epithelial integrity or in cytokine, LL-37 and NO production. Treatment of PCD epithelia using exogenous NO and antibiotic significantly reduced NTHi viability in biofilms compared with antibiotic treatment alone.<\/jats:p><jats:p>Impaired ciliary function was the primary defect in PCD airway epithelium underlying susceptibility to NTHi biofilm development compared with non-PCD epithelium. Although NO responses were similar, use of targeted NO with antibiotics enhanced killing of NTHi in biofilms, suggesting a novel therapeutic approach.<\/jats:p>","DOI":"10.1183\/13993003.00612-2017","type":"journal-article","created":{"date-parts":[[2017,11,10]],"date-time":"2017-11-10T09:52:28Z","timestamp":1510307548000},"page":"1700612","update-policy":"https:\/\/doi.org\/10.1183\/ers-crossmark-policy","source":"Crossref","is-referenced-by-count":43,"title":["Primary ciliary dyskinesia ciliated airway\u00a0cells show increased susceptibility to<i>Haemophilus influenzae<\/i>biofilm formation"],"prefix":"10.1183","volume":"50","author":[{"given":"Woolf T.","family":"Walker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire L.","family":"Jackson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond N.","family":"Allan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1571-7410","authenticated-orcid":false,"given":"Samuel\u00a0A.","family":"Collins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Kelso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ardeshir","family":"Rineh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nageshwar R.","family":"Yepuri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Nicholas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurie","family":"Lau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Johnston","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Lackie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saul N.","family":"Faust","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8701-9975","authenticated-orcid":false,"given":"Jane S.A.","family":"Lucas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luanne","family":"Hall-Stoodley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"81","published-online":{"date-parts":[[2017,9,10]]},"reference":[{"key":"2024101904235607000_50.3.1700612.1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00736-2016"},{"key":"2024101904235607000_50.3.1700612.2","doi-asserted-by":"crossref","first-page":"1093 e1091","DOI":"10.1016\/j.cmi.2015.08.020","article-title":"A longitudinal study of lung bacterial pathogens in patients with primary ciliary dyskinesia","volume":"21","author":"Alanin","year":"2015","journal-title":"Clin Microbiol Infect"},{"key":"2024101904235607000_50.3.1700612.3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0150949"},{"key":"2024101904235607000_50.3.1700612.4","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.7.4249-4260.2004"},{"key":"2024101904235607000_50.3.1700612.5","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.6.3210-3218.2005"},{"key":"2024101904235607000_50.3.1700612.6","doi-asserted-by":"publisher","DOI":"10.1001\/jama.296.2.202"},{"key":"2024101904235607000_50.3.1700612.7","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00756-10"},{"key":"2024101904235607000_50.3.1700612.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2014.10.024"},{"key":"2024101904235607000_50.3.1700612.9","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5418.1318"},{"key":"2024101904235607000_50.3.1700612.10","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.129.6.634"},{"key":"2024101904235607000_50.3.1700612.11","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200509-1459OC"},{"key":"2024101904235607000_50.3.1700612.12","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e31806009b0"},{"key":"2024101904235607000_50.3.1700612.13","doi-asserted-by":"crossref","unstructured":"Marsh RL , Thornton RB , Smith-Vaughan HC , et\u00a0al. 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