{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T01:17:44Z","timestamp":1790471864306,"version":"4.1.0"},"reference-count":37,"publisher":"European Respiratory Society (ERS)","issue":"1","funder":[{"name":"Stanford Translational Therapeutics Development Center"},{"name":"Science Foundation of Ireland","award":["SFI\/08\/US\/B1676"],"award-info":[{"award-number":["SFI\/08\/US\/B1676"]}]},{"name":"CFRI","award":["Elizabeth Nash Memorial Fellowship"],"award-info":[{"award-number":["Elizabeth Nash Memorial Fellowship"]}]},{"name":"the Ross Mosier Fund for CF Research"},{"DOI":"10.13039\/100004328","name":"Genentech","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004328","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006501","name":"Cystic Fibrosis Foundation Therapeutics","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006501","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["publications.ersnet.org"],"crossmark-restriction":true},"short-container-title":["Eur Respir J"],"accepted":{"date-parts":[[2015,8,14]]},"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:p>The diagnosis of cystic fibrosis (CF) patients with allergic bronchopulmonary aspergillosis (ABPA) is clinically challenging, due to the absence of an objective biological test. Since blood basophils play a major role in allergic responses, we hypothesised that changes in their surface activation pattern discriminate between CF patients with and without ABPA.<\/jats:p><jats:p>We conducted a prospective longitudinal study (Stanford cohort) comparing basophil activation test CD203c levels by flow cytometry before and after activation with<jats:italic>Aspergillus fumigatus<\/jats:italic>allergen extract or recombinant<jats:italic>Asp<\/jats:italic>f1 in 20 CF patients with ABPA (CF-ABPA) and in two comparison groups: CF patients with<jats:italic>A. fumigatus<\/jats:italic>colonisation (AC) but without ABPA (CF-AC; n=13) and CF patients without either AC or ABPA (CF; n=12). Patients were tested every 6\u2005months and when ill with pulmonary exacerbation. We also conducted cross-sectional validation in a separate patient set (Dublin cohort).<\/jats:p><jats:p>Basophil CD203c surface expression reliably discriminated CF-ABPA from CF-AC and CF over time<jats:italic>. Ex vivo<\/jats:italic>stimulation with<jats:italic>A. fumigatus<\/jats:italic>extract or recombinant<jats:italic>Asp<\/jats:italic>f1 produced similar results within the Stanford (p&lt;0.0001) and the Dublin cohorts. CF-ABPA patients were likelier to have elevated specific IgE to<jats:italic>A. fumigatus<\/jats:italic>and were less frequently co-infected with<jats:italic>Staphylococcus aureus<\/jats:italic>.<\/jats:p><jats:p>Basophil CD203c upregulation is a suitable diagnostic and stable monitoring biomarker of ABPA in CF.<\/jats:p>","DOI":"10.1183\/13993003.01068-2015","type":"journal-article","created":{"date-parts":[[2015,11,25]],"date-time":"2015-11-25T06:06:27Z","timestamp":1448431587000},"page":"177-185","update-policy":"https:\/\/doi.org\/10.1183\/ers-crossmark-policy","source":"Crossref","is-referenced-by-count":47,"title":["Blood basophil activation is a reliable biomarker of allergic bronchopulmonary aspergillosis in cystic 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