{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T01:58:25Z","timestamp":1790128705608,"version":"4.0.1"},"reference-count":45,"publisher":"European Respiratory Society (ERS)","issue":"5","license":[{"start":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T00:00:00Z","timestamp":1753920000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005927","name":"Daiichi Sankyo Foundation of Life Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005927","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007263","name":"Astellas Foundation for Research on Metabolic Disorders","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007263","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007428","name":"Naito Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007951","name":"Tokyo Medical and Dental University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007951","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18K15919"],"award-info":[{"award-number":["18K15919"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20K08515"],"award-info":[{"award-number":["20K08515"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21K18255"],"award-info":[{"award-number":["21K18255"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22H02845"],"award-info":[{"award-number":["22H02845"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22K007115"],"award-info":[{"award-number":["22K007115"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22K08230"],"award-info":[{"award-number":["22K08230"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23KJ0837"],"award-info":[{"award-number":["23KJ0837"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K11338"],"award-info":[{"award-number":["24K11338"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100024224","name":"Chemo-Sero-Therapeutic Research Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100024224","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008667","name":"SENSHIN Medical Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008667","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006680","name":"Takeda Medical Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006680","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020962","name":"ACT-X","doi-asserted-by":"publisher","award":["JPMJAX232I"],"award-info":[{"award-number":["JPMJAX232I"]}],"id":[{"id":"10.13039\/501100020962","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["publications.ersnet.org"],"crossmark-restriction":true},"short-container-title":["Eur Respir J"],"accepted":{"date-parts":[[2025,6,27]]},"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Acute respiratory distress syndrome (ARDS) is a severe form of lung failure with a high mortality rate and no effective pharmacological therapy. Although the cellular and molecular mechanisms involved in ARDS onset have been extensively studied, those governing its resolution remain unclear. Recent human cohort studies have suggested an association between ARDS severity and low blood basophil count. Therefore, in this study, we investigated the roles of basophils in ARDS pathogenesis and resolution.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We examined the effects of basophil depletion in lipopolysaccharide-induced ARDS model mice and assessed the roles of basophils in ARDS onset and resolution using genetically engineered mice and single-cell RNA-sequencing analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Intratracheal administration of lipopolysaccharides induced severe lung inflammation, characterised by extensive neutrophil infiltration, followed by gradual recovery to homeostatic conditions. Basophil depletion impaired the resolution but not the induction of lung inflammation, highlighting the critical role of basophils in the resolution phase of ARDS. Basophils accumulated in the lungs were the primary sources of the cytokine interleukin (IL)-4. Mice with basophil-specific IL-4 deficiency failed to resolve lung inflammation, as did mice with neutrophil-specific IL-4 receptor deficiency. Transcriptomic analysis revealed that basophil-derived IL-4 acted on neutrophils to suppress the anti-apoptotic gene and pro-inflammatory mediator expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Overall, our findings revealed that basophils played essential roles in the ARDS resolution phase, primarily by producing IL-4, which acted on neutrophils to alleviate lung inflammation in ARDS model mice.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1183\/13993003.01150-2024","type":"journal-article","created":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T15:05:12Z","timestamp":1753974312000},"page":"2401150","update-policy":"https:\/\/doi.org\/10.1183\/ers-crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Emerging roles of basophils in the resolution of acute respiratory distress syndrome"],"prefix":"10.1183","volume":"66","author":[{"given":"Seiko","family":"Takasawa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoya","family":"Tateishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Sugihara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sho","family":"Shibata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junya","family":"Ito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaru","family":"Ito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koki","family":"Hata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachiko","family":"Miyake","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajime","family":"Karasuyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1149-3755","authenticated-orcid":false,"given":"Kensuke","family":"Miyake","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9073-9815","authenticated-orcid":false,"given":"Yasunari","family":"Miyazaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"81","published-online":{"date-parts":[[2025,7,31]]},"reference":[{"key":"2025121603400963000_66.5.2401150.1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.202303-0558WS"},{"key":"2025121603400963000_66.5.2401150.2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1608077"},{"key":"2025121603400963000_66.5.2401150.3","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1038\/s41572-019-0069-0","article-title":"Acute respiratory distress syndrome","volume":"5","author":"Matthay","year":"2019","journal-title":"Nat Rev Dis Primers"},{"key":"2025121603400963000_66.5.2401150.4","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(22)01485-4"},{"key":"2025121603400963000_66.5.2401150.5","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1016\/S0140-6736(22)01439-8","article-title":"Acute respiratory distress syndrome in adults: diagnosis, outcomes, long-term sequelae, and management","volume":"400","author":"Gorman","year":"2022","journal-title":"Lancet"},{"key":"2025121603400963000_66.5.2401150.6","doi-asserted-by":"publisher","DOI":"10.1002\/path.5221"},{"key":"2025121603400963000_66.5.2401150.7","doi-asserted-by":"publisher","DOI":"10.1086\/338122"},{"key":"2025121603400963000_66.5.2401150.8","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.jmii.2019.07.009","article-title":"Anti-IFN-\u03b3 therapy alleviates acute lung injury induced by severe influenza A (H1N1) pdm09 infection in mice","volume":"54","author":"Liu","year":"2021","journal-title":"J Microbiol Immunol Infect"},{"key":"2025121603400963000_66.5.2401150.9","doi-asserted-by":"crossref","first-page":"e14368","DOI":"10.14814\/phy2.14368","article-title":"Effects of IFN-\u03b3 on immune cell kinetics during the resolution of acute lung injury","volume":"8","author":"Mock","year":"2020","journal-title":"Physiol Rep"},{"key":"2025121603400963000_66.5.2401150.10","doi-asserted-by":"crossref","unstructured":"D'Alessio FR . Mouse models of acute lung injury and ARDS. In: Alper S , Janssen WJ , eds. Lung Innate Immunity and Inflammation: Methods and Protocols. New York, Springer New York, 2018; pp. 341\u2013350.","DOI":"10.1007\/978-1-4939-8570-8_22"},{"key":"2025121603400963000_66.5.2401150.11","doi-asserted-by":"crossref","first-page":"18533","DOI":"10.1038\/s41598-023-45865-y","article-title":"Basophils absence predicts poor prognosis and indicates immunosuppression of patients in intensive care units","volume":"13","author":"Chen","year":"2023","journal-title":"Sci Rep"},{"key":"2025121603400963000_66.5.2401150.12","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-1038-6"},{"key":"2025121603400963000_66.5.2401150.13","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2109123118"},{"key":"2025121603400963000_66.5.2401150.14","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2018.06.006"},{"key":"2025121603400963000_66.5.2401150.15","doi-asserted-by":"publisher","DOI":"10.1111\/all.14662"},{"key":"2025121603400963000_66.5.2401150.16","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2021.101529"},{"key":"2025121603400963000_66.5.2401150.17","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1056838"},{"key":"2025121603400963000_66.5.2401150.18","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-46148-4"},{"key":"2025121603400963000_66.5.2401150.19","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.12.020"},{"key":"2025121603400963000_66.5.2401150.20","doi-asserted-by":"publisher","DOI":"10.1172\/JCI136778"},{"key":"2025121603400963000_66.5.2401150.21","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/j.jaci.2021.02.018","article-title":"Basophils promote barrier dysfunction and resolution in the atopic skin","volume":"148","author":"Pellefigues","year":"2021","journal-title":"J Allergy Clin Immunol"},{"key":"2025121603400963000_66.5.2401150.22","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00419.2015"},{"key":"2025121603400963000_66.5.2401150.23","doi-asserted-by":"publisher","DOI":"10.1172\/JCI42680"},{"key":"2025121603400963000_66.5.2401150.24","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(01)00084-X"},{"key":"2025121603400963000_66.5.2401150.25","doi-asserted-by":"publisher","DOI":"10.1038\/362245a0"},{"key":"2025121603400963000_66.5.2401150.26","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1813927115"},{"key":"2025121603400963000_66.5.2401150.27","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(04)00107-4"},{"key":"2025121603400963000_66.5.2401150.28","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.10.010"},{"key":"2025121603400963000_66.5.2401150.29","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2012.12.001"},{"key":"2025121603400963000_66.5.2401150.30","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2009-0210ST"},{"key":"2025121603400963000_66.5.2401150.31","doi-asserted-by":"publisher","DOI":"10.1038\/S41587-023-01767-Y"},{"key":"2025121603400963000_66.5.2401150.32","first-page":"100141","article-title":"clusterProfiler 4.0: a universal enrichment tool for interpreting omics data","volume":"2","author":"Wu","year":"2021","journal-title":"Innovation"},{"key":"2025121603400963000_66.5.2401150.33","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00010.2008"},{"key":"2025121603400963000_66.5.2401150.34","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-01-068718"},{"key":"2025121603400963000_66.5.2401150.35","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1038\/s41419-019-1900-y","article-title":"Efficacy of pulmonary transplantation of engineered macrophages secreting IL-4 on acute lung injury in C57BL\/6J mice","volume":"10","author":"Liu","year":"2019","journal-title":"Cell Death Dis"},{"key":"2025121603400963000_66.5.2401150.36","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201808-1599OC"},{"key":"2025121603400963000_66.5.2401150.37","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00170.2004"},{"key":"2025121603400963000_66.5.2401150.38","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2004-0330OC"},{"key":"2025121603400963000_66.5.2401150.39","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathmechdis-012418-012718"},{"key":"2025121603400963000_66.5.2401150.40","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2016.02.015"},{"key":"2025121603400963000_66.5.2401150.41","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12216"},{"key":"2025121603400963000_66.5.2401150.42","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20220525"},{"key":"2025121603400963000_66.5.2401150.43","doi-asserted-by":"publisher","DOI":"10.1126\/science.aai8132"},{"key":"2025121603400963000_66.5.2401150.44","doi-asserted-by":"crossref","first-page":"195","DOI":"10.3390\/jpm11030195","article-title":"White blood cells and severe COVID-19: a mendelian randomization study","volume":"11","author":"Sun","year":"2021","journal-title":"J Pers Med"},{"key":"2025121603400963000_66.5.2401150.45","doi-asserted-by":"crossref","first-page":"100078","DOI":"10.1016\/j.xcrm.2020.100078","article-title":"Systems-level immunomonitoring from acute to recovery phase of severe COVID-19","volume":"1","author":"Rodriguez","year":"2020","journal-title":"Cell Rep Med"}],"container-title":["European Respiratory Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1183\/13993003.01150-2024","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T11:40:21Z","timestamp":1765885221000},"score":1,"resource":{"primary":{"URL":"https:\/\/publications.ersnet.org\/lookup\/doi\/10.1183\/13993003.01150-2024"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,31]]},"references-count":45,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2025,11,6]]},"published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1183\/13993003.01150-2024"],"URL":"https:\/\/doi.org\/10.1183\/13993003.01150-2024","relation":{},"ISSN":["0903-1936","1399-3003"],"issn-type":[{"value":"0903-1936","type":"print"},{"value":"1399-3003","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,31]]}}}