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Mutation frequencies in resistant and susceptible isolates were calculated, together with novel statistical measures to classify mutations as high, moderate, minimal or indeterminate confidence for predicting resistance.<\/jats:p>\n                  <jats:p>We identified 286 confidence-graded mutations associated with resistance. Compared to phenotypic methods, sensitivity (95% CI) for rifampicin was 90.3% (89.6\u201390.9%), while for isoniazid it was 78.2% (77.4\u201379.0%) and their specificities were 96.3% (95.7\u201396.8%) and 94.4% (93.1\u201395.5%), respectively. For second-line drugs, sensitivity varied from 67.4% (64.1\u201370.6%) for capreomycin to 88.2% (85.1\u201390.9%) for moxifloxacin, with specificity ranging from 90.0% (87.1\u201392.5%) for moxifloxacin to 99.5% (99.0\u201399.8%) for amikacin.<\/jats:p>\n                  <jats:p>\n                    This study provides a standardised and comprehensive approach for the interpretation of mutations as predictors of\n                    <jats:italic>M. tuberculosis<\/jats:italic>\n                    drug-resistant phenotypes. These data have implications for the clinical interpretation of molecular diagnostics and next-generation sequencing as well as efficient individualised therapy for patients with drug-resistant tuberculosis.\n                  <\/jats:p>","DOI":"10.1183\/13993003.01354-2017","type":"journal-article","created":{"date-parts":[[2017,12,28]],"date-time":"2017-12-28T20:10:41Z","timestamp":1514491841000},"page":"1701354","update-policy":"https:\/\/doi.org\/10.1183\/ers-crossmark-policy","source":"Crossref","is-referenced-by-count":322,"title":["A standardised method for interpreting the association between mutations and phenotypic drug resistance in\n                    <i>Mycobacterium 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