{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:55:12Z","timestamp":1791593712149,"version":"4.3.4"},"reference-count":32,"publisher":"European Respiratory Society (ERS)","issue":"2","funder":[{"DOI":"10.13039\/100009148","name":"Queen Mary University of London","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009148","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000836","name":"University of Liverpool","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000836","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["publications.ersnet.org"],"crossmark-restriction":true},"short-container-title":["Eur Respir J"],"accepted":{"date-parts":[[2017,11,20]]},"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:p>E-cigarette vapour contains free radicals with the potential to induce oxidative stress. Since oxidative stress in airway cells increases platelet-activating factor receptor (PAFR) expression, and PAFR is co-opted by pneumococci to adhere to host cells, we hypothesised that E-cigarette vapour increases pneumococcal adhesion to airway cells.<\/jats:p><jats:p>Nasal epithelial PAFR was assessed in non-vaping controls, and in adults before and after 5\u2005min of vaping. We determined the effect of vapour on oxidative stress-induced, PAFR-dependent pneumococcal adhesion to airway epithelial cells<jats:italic>in vitro<\/jats:italic>, and on pneumococcal colonisation in the mouse nasopharynx. Elemental analysis of vapour was done by mass spectrometry, and oxidative potential of vapour assessed by antioxidant depletion<jats:italic>in vitro<\/jats:italic>.<\/jats:p><jats:p>There was no difference in baseline nasal epithelial PAFR expression between vapers (n=11) and controls (n=6). Vaping increased nasal PAFR expression. Nicotine-containing and nicotine-free E-cigarette vapour increased pneumococcal adhesion to airway cells<jats:italic>in vitro<\/jats:italic>. Vapour-stimulated adhesion<jats:italic>in vitro<\/jats:italic>was attenuated by the PAFR blocker CV3988. Nicotine-containing E-cigarette vapour increased mouse nasal PAFR expression, and nasopharyngeal pneumococcal colonisation. Vapour contained redox-active metals, had considerable oxidative activity, and adhesion was attenuated by the antioxidant N-acetyl cysteine.<\/jats:p><jats:p>This study suggests that E-cigarette vapour has the potential to increase susceptibility to pneumococcal infection.<\/jats:p>","DOI":"10.1183\/13993003.01592-2017","type":"journal-article","created":{"date-parts":[[2018,2,8]],"date-time":"2018-02-08T10:08:45Z","timestamp":1518084525000},"page":"1701592","update-policy":"https:\/\/doi.org\/10.1183\/ers-crossmark-policy","source":"Crossref","is-referenced-by-count":117,"title":["E-cigarette vapour enhances pneumococcal adherence to airway epithelial cells"],"prefix":"10.1183","volume":"51","author":[{"given":"Lisa","family":"Miyashita","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5509-2502","authenticated-orcid":false,"given":"Reetika","family":"Suri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Dearing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"Mudway","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosamund\u00a0E","family":"Dove","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel R.","family":"Neill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4115-5362","authenticated-orcid":false,"given":"Richard","family":"Van Zyl-Smit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aras","family":"Kadioglu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3109-6028","authenticated-orcid":false,"given":"Jonathan","family":"Grigg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"81","published-online":{"date-parts":[[2018,2,7]]},"reference":[{"key":"2024101909294817000_51.2.1701592.1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200003093421002"},{"key":"2024101909294817000_51.2.1701592.2","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2008.106385"},{"key":"2024101909294817000_51.2.1701592.3","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1289\/ehp.1306755","article-title":"Air pollution and respiratory infections during early childhood: an analysis of 10 European birth cohorts within the ESCAPE Project","volume":"122","author":"MacIntyre","year":"2014","journal-title":"Environ Health Perspect"},{"key":"2024101909294817000_51.2.1701592.4","doi-asserted-by":"publisher","DOI":"10.1378\/chest.12-0210"},{"key":"2024101909294817000_51.2.1701592.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2010.02.2268"},{"key":"2024101909294817000_51.2.1701592.6","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1016\/j.jaci.2016.04.062","article-title":"Airborne dust and high temperatures are risk factors for invasive bacterial disease","volume":"139","author":"Jusot","year":"2017","journal-title":"J Allergy Clin Immunol"},{"key":"2024101909294817000_51.2.1701592.7","doi-asserted-by":"crossref","first-page":"3878","DOI":"10.1172\/JCI81888","article-title":"Bacterial exploitation of phosphorylcholine mimicry suppresses inflammation to promote airway infection","volume":"125","author":"Hergott","year":"2015","journal-title":"J Clin Invest"},{"key":"2024101909294817000_51.2.1701592.8","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2011-200835"},{"key":"2024101909294817000_51.2.1701592.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.11.039"},{"key":"2024101909294817000_51.2.1701592.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.06.033"},{"key":"2024101909294817000_51.2.1701592.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.ypmed.2014.10.009"},{"key":"2024101909294817000_51.2.1701592.12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0116732"},{"key":"2024101909294817000_51.2.1701592.13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0116861"},{"key":"2024101909294817000_51.2.1701592.14","unstructured":"abcam. 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