{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T12:18:28Z","timestamp":1790338708776,"version":"4.1.0"},"reference-count":40,"publisher":"European Respiratory Society (ERS)","issue":"5","funder":[{"DOI":"10.13039\/501100004744","name":"Innoviris","doi-asserted-by":"publisher","award":["Doctiris program"],"award-info":[{"award-number":["Doctiris program"]}],"id":[{"id":"10.13039\/501100004744","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["publications.ersnet.org"],"crossmark-restriction":true},"short-container-title":["Eur Respir J"],"accepted":{"date-parts":[[2017,1,15]]},"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:p>Cystic fibrosis (CF) patients receive chronic treatment with macrolides for their antivirulence and anti-inflammatory properties. We, however, previously showed that<jats:italic>Pseudomonas aeruginosa<\/jats:italic>, considered as naturally resistant to macrolides, becomes susceptible when tested in a eukaryotic medium rather than a conventional broth.<\/jats:p><jats:p>We therefore looked for specific macrolide resistance determinants in 333 CF isolates from four European CF centres in comparison with 48 isolates from patients suffering from hospital-acquired pneumonia (HAP).<\/jats:p><jats:p>Minimum inhibitory concentrations (MICs) of macrolides and ketolides measured in eukaryotic medium (RPMI-1640) were higher towards CF than HAP isolates. Gene sequencing revealed mutations at three positions (2045, 2046 and 2598) in domain V of 23S rRNA of 43% of sequenced CF isolates, but none in HAP isolates. Enzymes degrading extracellular polymeric substances also reduced MICs, highlighting a role of the mucoid, biofilm-forming phenotype in resistance. An association between high MICs and chronic azithromycin administration was evidenced, which was statistically significant for patients infected by the Liverpool Epidemic Strain.<\/jats:p><jats:p>Thus, ribosomal mutations are highly prevalent in CF isolates and may spread in epidemic clones, arguing for prudent use of oral macrolides in these patients. Measuring MICs in RPMI-1640 could be easily implemented in microbiology laboratories to phenotypically detect resistance.<\/jats:p>","DOI":"10.1183\/13993003.01847-2016","type":"journal-article","created":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T20:08:22Z","timestamp":1495570102000},"page":"1601847","update-policy":"https:\/\/doi.org\/10.1183\/ers-crossmark-policy","source":"Crossref","is-referenced-by-count":51,"title":["Acquired resistance to macrolides in<i>Pseudomonas aeruginosa<\/i>from cystic fibrosis patients"],"prefix":"10.1183","volume":"49","author":[{"given":"Muhammad-Hariri","family":"Mustafa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaunak","family":"Khandekar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael M.","family":"Tunney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u00a0Stuart","family":"Elborn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara C.","family":"Kahl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Denis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Pl\u00e9siat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamidou","family":"Traore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul M.","family":"Tulkens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"Vanderbist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Van\u00a0Bambeke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"81","published-online":{"date-parts":[[2017,5,19]]},"reference":[{"key":"2024101903510551000_49.5.1601847.1","unstructured":"Cystic Fibrosis Foundation. Patient registry \u2013 annual data report. 2015. www.cff.org\/Our-Research\/CF-Patient-Registry\/2015-Patient-Registry-Annual-Data-Report.pdf Date last accessed: February 25, 2017."},{"key":"2024101903510551000_49.5.1601847.2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00178"},{"key":"2024101903510551000_49.5.1601847.3","doi-asserted-by":"publisher","DOI":"10.1007\/s00228-011-1161-x"},{"key":"2024101903510551000_49.5.1601847.4","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD002203.pub4"},{"key":"2024101903510551000_49.5.1601847.5","unstructured":"Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; 25th Informational Supplement. CLSI document M100-S25. Wayne, CLSI, 2015."},{"key":"2024101903510551000_49.5.1601847.6","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.12.3322-3327.2000"},{"key":"2024101903510551000_49.5.1601847.7","first-page":"48","article-title":"Efflux as a mechanism of resistance to antimicrobials in Pseudomonas aeruginosa and related bacteria: unanswered questions","volume":"2","author":"Schweizer","year":"2003","journal-title":"Genet Mol Res"},{"key":"2024101903510551000_49.5.1601847.8","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis473"},{"key":"2024101903510551000_49.5.1601847.9","doi-asserted-by":"publisher","DOI":"10.1086\/324626"},{"key":"2024101903510551000_49.5.1601847.10","doi-asserted-by":"publisher","DOI":"10.1007\/s101560050011"},{"key":"2024101903510551000_49.5.1601847.11","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00630-12"},{"key":"2024101903510551000_49.5.1601847.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2010.08.005"},{"key":"2024101903510551000_49.5.1601847.13","doi-asserted-by":"publisher","DOI":"10.3109\/07853890.2014.935470"},{"key":"2024101903510551000_49.5.1601847.14","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01841.x"},{"key":"2024101903510551000_49.5.1601847.15","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00860-10"},{"key":"2024101903510551000_49.5.1601847.16","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.2016.1138857"},{"key":"2024101903510551000_49.5.1601847.17","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)00017-7"},{"key":"2024101903510551000_49.5.1601847.18","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)05169-0"},{"key":"2024101903510551000_49.5.1601847.19","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01046-16"},{"key":"2024101903510551000_49.5.1601847.20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0060666"},{"key":"2024101903510551000_49.5.1601847.21","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.26.4.546"},{"key":"2024101903510551000_49.5.1601847.22","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.1.1-12.2001"},{"key":"2024101903510551000_49.5.1601847.23","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/mr.58.1.10-26.1994","article-title":"Lessons from an evolving rRNA: 16S and 23S rRNA structures from a comparative perspective","volume":"58","author":"Gutell","year":"1994","journal-title":"Microbiol Rev"},{"key":"2024101903510551000_49.5.1601847.24","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki249"},{"key":"2024101903510551000_49.5.1601847.25","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2011.00322.x"},{"key":"2024101903510551000_49.5.1601847.26","doi-asserted-by":"publisher","DOI":"10.1101\/gr.086082.108"},{"key":"2024101903510551000_49.5.1601847.27","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2011.11.004"},{"key":"2024101903510551000_49.5.1601847.28","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0071001"},{"key":"2024101903510551000_49.5.1601847.29","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.015875-0"},{"key":"2024101903510551000_49.5.1601847.30","first-page":"60","article-title":"Cross-infections with Pseudomonas aeruginosa in patients with cystic fibrosis attending the Warsaw Centre","volume":"19","author":"Milczewska","year":"2015","journal-title":"Dev Period Med"},{"key":"2024101903510551000_49.5.1601847.31","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.57.11.921"},{"key":"2024101903510551000_49.5.1601847.32","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.2207005"},{"key":"2024101903510551000_49.5.1601847.33","doi-asserted-by":"publisher","DOI":"10.4187\/aarc0446"},{"key":"2024101903510551000_49.5.1601847.34","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.1.57-71.2004"},{"key":"2024101903510551000_49.5.1601847.35","doi-asserted-by":"crossref","first-page":"317","DOI":"10.3389\/fmicb.2016.00317","article-title":"PA3297 counteracts antimicrobial effects of azithromycin in Pseudomonas aeruginosa","volume":"7","author":"Tan","year":"2016","journal-title":"Front Microbiol"},{"key":"2024101903510551000_49.5.1601847.36","doi-asserted-by":"publisher","DOI":"10.1086\/324527"},{"key":"2024101903510551000_49.5.1601847.37","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku452"},{"key":"2024101903510551000_49.5.1601847.38","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/5584_2014_50","article-title":"Inhalation of macrolides: a novel approach to treatment of pulmonary infections","volume":"839","author":"Siekmeier","year":"2015","journal-title":"Adv Exp Med Biol"},{"key":"2024101903510551000_49.5.1601847.39","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.112.200733"},{"key":"2024101903510551000_49.5.1601847.40","first-page":"289","article-title":"Anti-inflammatory effects of macrolides in a model relevant to CF airway epithelial inflammation","volume":"46","author":"Ribeiro","year":"2011","journal-title":"Pediatr Pulmonol"}],"container-title":["European Respiratory Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1183\/13993003.01847-2016","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T06:40:59Z","timestamp":1740379259000},"score":1,"resource":{"primary":{"URL":"https:\/\/publications.ersnet.org\/lookup\/doi\/10.1183\/13993003.01847-2016"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5]]},"references-count":40,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2017,5,19]]},"published-print":{"date-parts":[[2017,5]]}},"alternative-id":["10.1183\/13993003.01847-2016"],"URL":"https:\/\/doi.org\/10.1183\/13993003.01847-2016","relation":{},"ISSN":["0903-1936","1399-3003"],"issn-type":[{"value":"0903-1936","type":"print"},{"value":"1399-3003","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5]]}}}