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This study examined the effect of exogenous and endogenous oxidative stress on macrophage phagocytosis in patients with COPD.<\/jats:p>\n                  <jats:p>\n                    Monocyte-derived macrophages (MDMs) were generated from non-smoker, smoker and COPD subjects, differentiated in either granulocyte macrophage-colony stimulating factor (G-M\u03c6) or macrophage-colony stimulating factor (M-M\u03c6). Alveolar macrophages were isolated from lung tissue or bronchoalveolar lavage fluid. Macrophages were incubated in \u00b1200\u2005\u00b5M H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    for 24\u2005h, then exposed to fluorescently labelled\n                    <jats:italic>Haemophilus influenzae<\/jats:italic>\n                    or\n                    <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n                    for 4\u2005h, after which phagocytosis, mitochondrial ROS (mROS) and mitochondrial membrane potential (\u0394\u03a8m) were measured.\n                  <\/jats:p>\n                  <jats:p>Phagocytosis of bacteria was significantly decreased in both G-M\u03c6 and M-M\u03c6 from COPD patients compared with from non-smoker controls. In non-smokers and smokers, bacterial phagocytosis did not alter mROS or \u0394\u03a8m; however, in COPD, phagocytosis increased early mROS and decreased \u0394\u03a8m in both G-M\u03c6 and M-M\u03c6. Exogenous oxidative stress reduced phagocytosis in non-smoker and COPD alveolar macrophages and non-smoker MDMs, associated with reduced mROS production.<\/jats:p>\n                  <jats:p>COPD macrophages show defective phagocytosis, which is associated with altered mitochondrial function and an inability to regulate mROS production. Targeting mitochondrial dysfunction may restore the phagocytic defect in COPD.<\/jats:p>","DOI":"10.1183\/13993003.02244-2018","type":"journal-article","created":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T08:26:05Z","timestamp":1563438365000},"page":"1802244","update-policy":"https:\/\/doi.org\/10.1183\/ers-crossmark-policy","source":"Crossref","is-referenced-by-count":131,"title":["Defective bacterial phagocytosis is associated with dysfunctional mitochondria in COPD macrophages"],"prefix":"10.1183","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3241-258X","authenticated-orcid":false,"given":"Kylie B.R.","family":"Belchamber","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richa","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig 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