{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T19:21:55Z","timestamp":1792092115950,"version":"4.4.0"},"reference-count":69,"publisher":"European Respiratory Society (ERS)","issue":"1","license":[{"start":{"date-parts":[[2022,5,20]],"date-time":"2022-05-20T00:00:00Z","timestamp":1653004800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.ersjournals.com\/user-licence"}],"content-domain":{"domain":["publications.ersnet.org"],"crossmark-restriction":true},"short-container-title":["Eur Respir J"],"accepted":{"date-parts":[[2022,4,19]]},"published-print":{"date-parts":[[2022,7]]},"DOI":"10.1183\/13993003.02300-2021","type":"journal-article","created":{"date-parts":[[2022,5,23]],"date-time":"2022-05-23T16:26:06Z","timestamp":1653323166000},"page":"2102300","update-policy":"https:\/\/doi.org\/10.1183\/ers-crossmark-policy","source":"Crossref","is-referenced-by-count":18,"title":["Methods for the assessment of human airway ciliary function"],"prefix":"10.1183","volume":"60","author":[{"given":"Claire L.","family":"Jackson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Bottier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"81","published-online":{"date-parts":[[2022,5,20]]},"reference":[{"key":"2024102102580032000_60.1.2102300.1","doi-asserted-by":"publisher","DOI":"10.1002\/path.4843"},{"key":"2024102102580032000_60.1.2102300.2","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/S2213-2600(21)00453-7","article-title":"The global prevalence and ethnic heterogeneity of primary ciliary dyskinesia gene variants: a genetic database analysis","volume":"10","author":"Hannah","year":"2022","journal-title":"Lancet Respir Med"},{"key":"2024102102580032000_60.1.2102300.3","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1111\/cpf.12085","article-title":"Mucociliary clearance: pathophysiological aspects","volume":"34","author":"Munkholm","year":"2014","journal-title":"Clin Physiol Funct Imaging"},{"key":"2024102102580032000_60.1.2102300.4","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/S2213-2600(19)30374-1","article-title":"Primary ciliary dyskinesia in the genomics age","volume":"8","author":"Lucas","year":"2020","journal-title":"Lancet Respir Med"},{"key":"2024102102580032000_60.1.2102300.5","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.02359-2020"},{"key":"2024102102580032000_60.1.2102300.6","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1164\/ajrccm.151.5.7735615","article-title":"Ciliary defects in healthy subjects, bronchiectasis, and primary ciliary dyskinesia","volume":"151","author":"De Iongh","year":"1995","journal-title":"Am J Respir Crit Care Med"},{"key":"2024102102580032000_60.1.2102300.7","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1080\/15412555.2021.1963695","article-title":"Dysfunctional bronchial cilia are a feature of chronic obstructive pulmonary disease (COPD)","volume":"18","author":"Thomas","year":"2021","journal-title":"COPD"},{"key":"2024102102580032000_60.1.2102300.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.05.046"},{"key":"2024102102580032000_60.1.2102300.9","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2015-207588"},{"key":"2024102102580032000_60.1.2102300.10","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1177\/0003489419859376","article-title":"Direct visualization of laryngeal mucociliary clearance in adults","volume":"128","author":"O'Neil","year":"2019","journal-title":"Ann Otol Rhinol Laryngol"},{"key":"2024102102580032000_60.1.2102300.11","doi-asserted-by":"publisher","DOI":"10.1080\/00039896.1974.10666589"},{"key":"2024102102580032000_60.1.2102300.12","doi-asserted-by":"publisher","DOI":"10.1136\/thx.36.9.654"},{"key":"2024102102580032000_60.1.2102300.13","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00011814"},{"key":"2024102102580032000_60.1.2102300.14","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.2.002022"},{"key":"2024102102580032000_60.1.2102300.15","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.24.3.030601"},{"key":"2024102102580032000_60.1.2102300.16","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1518527113"},{"key":"2024102102580032000_60.1.2102300.17","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-3789-9_16"},{"key":"2024102102580032000_60.1.2102300.18","doi-asserted-by":"publisher","DOI":"10.1002\/humu.22001"},{"key":"2024102102580032000_60.1.2102300.19","doi-asserted-by":"crossref","first-page":"5791","DOI":"10.1016\/j.cell.2021.10.007","article-title":"De novo identification of mammalian ciliary motility proteins using cryo-EM","volume":"184","author":"Gui","year":"2021","journal-title":"Cell"},{"key":"2024102102580032000_60.1.2102300.20","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.1994.tb02537.x"},{"key":"2024102102580032000_60.1.2102300.21","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1002\/ppul.21402","article-title":"Diagnostic yield of nasal scrape biopsies in primary ciliary dyskinesia: a multicenter experience","volume":"46","author":"Olin","year":"2011","journal-title":"Pediatr Pulmonol"},{"key":"2024102102580032000_60.1.2102300.22","doi-asserted-by":"crossref","first-page":"3753","DOI":"10.3390\/jcm9113753","article-title":"A revised protocol for culture of airway epithelial cells as a diagnostic tool for primary ciliary dyskinesia","volume":"9","author":"Coles","year":"2020","journal-title":"J Clin Med"},{"key":"2024102102580032000_60.1.2102300.23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0089675"},{"key":"2024102102580032000_60.1.2102300.24","first-page":"497","article-title":"Ciliary beat frequency of human respiratory tract by different sampling techniques","volume":"130","author":"Low","year":"1984","journal-title":"Am Rev Respir Dis"},{"key":"2024102102580032000_60.1.2102300.25","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/76.6.854"},{"key":"2024102102580032000_60.1.2102300.26","doi-asserted-by":"crossref","first-page":"4896","DOI":"10.1038\/s41598-021-84605-y","article-title":"Differential effect of anesthetics on mucociliary clearance in vivo in mice","volume":"11","author":"Feldman","year":"2021","journal-title":"Sci Rep"},{"key":"2024102102580032000_60.1.2102300.27","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1080\/01913123.2017.1365990","article-title":"Secondary defects detected by transmission electron microscopy in primary ciliary dyskinesia diagnostics","volume":"41","author":"Dixon","year":"2017","journal-title":"Ultrastruct Pathol"},{"key":"2024102102580032000_60.1.2102300.28","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00725-2019"},{"key":"2024102102580032000_60.1.2102300.29","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00176608"},{"key":"2024102102580032000_60.1.2102300.30","doi-asserted-by":"crossref","first-page":"1601090","DOI":"10.1183\/13993003.01090-2016","article-title":"European Respiratory Society guidelines for the diagnosis of primary ciliary dyskinesia","volume":"49","author":"Lucas","year":"2017","journal-title":"Eur Respir"},{"key":"2024102102580032000_60.1.2102300.31","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00153308"},{"key":"2024102102580032000_60.1.2102300.32","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.55.4.314"},{"key":"2024102102580032000_60.1.2102300.33","doi-asserted-by":"publisher","DOI":"10.1038\/196592a0"},{"key":"2024102102580032000_60.1.2102300.34","doi-asserted-by":"publisher","DOI":"10.1136\/adc.66.4_Spec_No.443"},{"key":"2024102102580032000_60.1.2102300.35","doi-asserted-by":"publisher","DOI":"10.1378\/chest.10-1920"},{"key":"2024102102580032000_60.1.2102300.36","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1016\/j.chest.2019.01.036","article-title":"Accuracy of high-speed video analysis to diagnose primary ciliary dyskinesia","volume":"155","author":"Rubbo","year":"2019","journal-title":"Chest"},{"key":"2024102102580032000_60.1.2102300.37","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.3390\/diagnostics11071301","article-title":"High-speed video microscopy for primary ciliary dyskinesia diagnosis: a study of ciliary motility variations with time and temperature","volume":"11","author":"Reula","year":"2021","journal-title":"Diagnostics"},{"key":"2024102102580032000_60.1.2102300.38","doi-asserted-by":"publisher","DOI":"10.1378\/chest.11-3253"},{"key":"2024102102580032000_60.1.2102300.39","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.congress-2021.PA2362"},{"key":"2024102102580032000_60.1.2102300.40","first-page":"PA4003","article-title":"Automated time-lapse analysis of ciliary function","volume":"48","author":"Jackson","year":"2016","journal-title":"Eur Respir J"},{"key":"2024102102580032000_60.1.2102300.41","doi-asserted-by":"crossref","first-page":"4923","DOI":"10.3390\/ijms22094923","article-title":"Properties of non-aminoglycoside compounds used to stimulate translational readthrough of PTC mutations in primary ciliary dyskinesia","volume":"22","author":"Dabrowski","year":"2021","journal-title":"Int J Mol Sci"},{"key":"2024102102580032000_60.1.2102300.42","doi-asserted-by":"publisher","DOI":"10.1186\/2046-2530-1-S1-O14"},{"key":"2024102102580032000_60.1.2102300.43","doi-asserted-by":"publisher","DOI":"10.1183\/23120541.00792-2020"},{"key":"2024102102580032000_60.1.2102300.44","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00052014"},{"key":"2024102102580032000_60.1.2102300.45","doi-asserted-by":"publisher","DOI":"10.1378\/chest.10-0175"},{"key":"2024102102580032000_60.1.2102300.46","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/7651_2019_269","article-title":"Ciliated epithelial cell differentiation at air-liquid interface using commercially available culture media","volume":"2109","author":"Lee","year":"2020","journal-title":"Methods Mol Biol"},{"key":"2024102102580032000_60.1.2102300.47","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/s13630-017-0049-5","article-title":"Patient-specific three-dimensional explant spheroids derived from human nasal airway epithelium: a simple methodological approach for ex vivo studies of primary ciliary dyskinesia","volume":"6","author":"Marthin","year":"2017","journal-title":"Cilia"},{"key":"2024102102580032000_60.1.2102300.48","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3697"},{"key":"2024102102580032000_60.1.2102300.49","doi-asserted-by":"crossref","first-page":"1348","DOI":"10.1016\/j.chest.2017.06.053","article-title":"Exploring the art of ciliary beating: the benefits of high-speed video analysis","volume":"152","author":"Lucas","year":"2017","journal-title":"Chest"},{"key":"2024102102580032000_60.1.2102300.50","doi-asserted-by":"publisher","DOI":"10.1183\/16000617.0058-2016"},{"key":"2024102102580032000_60.1.2102300.51","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1016\/j.chest.2016.09.015","article-title":"Variation of ciliary beat pattern in three different beating planes in healthy subjects","volume":"151","author":"Kempeneers","year":"2017","journal-title":"Chest"},{"key":"2024102102580032000_60.1.2102300.52","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1002\/ppul.24439","article-title":"Ciliary functional analysis: beating a path towards standardization","volume":"54","author":"Kempeneers","year":"2019","journal-title":"Pediatr Pulmonol"},{"key":"2024102102580032000_60.1.2102300.53","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1172-7-78"},{"key":"2024102102580032000_60.1.2102300.54","doi-asserted-by":"publisher","DOI":"10.1136\/jmedgenet-2019-106424"},{"key":"2024102102580032000_60.1.2102300.55","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2017.05.028"},{"key":"2024102102580032000_60.1.2102300.56","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2017-0436OC"},{"key":"2024102102580032000_60.1.2102300.57","doi-asserted-by":"crossref","first-page":"1292","DOI":"10.1016\/j.bpj.2019.02.012","article-title":"How does cilium length affect beating?","volume":"116","author":"Bottier","year":"2019","journal-title":"Biophys J"},{"key":"2024102102580032000_60.1.2102300.58","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.1038\/s41467-019-09798-3","article-title":"Phenotyping ciliary dynamics and coordination in response to CFTR-modulators in cystic fibrosis respiratory epithelial cells","volume":"10","author":"Chioccioli","year":"2019","journal-title":"Nat Commun"},{"key":"2024102102580032000_60.1.2102300.59","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.1164\/rccm.201812-2256LE","article-title":"Quantitative high-speed video profiling discriminates between DNAH11 and HYDIN variants of primary ciliary dyskinesia","volume":"199","author":"Chioccioli","year":"2019","journal-title":"Am J Respir Crit Care Med"},{"key":"2024102102580032000_60.1.2102300.60","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1038\/mi.2012.81","article-title":"Molecular organization of the mucins and glycocalyx underlying mucus transport over mucosal surfaces of the airways","volume":"6","author":"Kesimer","year":"2013","journal-title":"Mucosal Immunology"},{"key":"2024102102580032000_60.1.2102300.61","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00321.2010"},{"key":"2024102102580032000_60.1.2102300.62","doi-asserted-by":"crossref","first-page":"e1005605","DOI":"10.1371\/journal.pcbi.1005605","article-title":"A new index for characterizing micro-bead motion in a flow induced by ciliary beating: Part I, experimental analysis","volume":"13","author":"Bottier","year":"2017","journal-title":"PLoS Comput Biol"},{"key":"2024102102580032000_60.1.2102300.63","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-20882-4"},{"key":"2024102102580032000_60.1.2102300.64","doi-asserted-by":"publisher","DOI":"10.1063\/1.4901930"},{"key":"2024102102580032000_60.1.2102300.65","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1016\/j.bpj.2021.01.041","article-title":"Induction of ciliary orientation by matrix patterning and characterization of mucociliary transport","volume":"120","author":"Sears","year":"2021","journal-title":"Biophysical Journal"},{"key":"2024102102580032000_60.1.2102300.66","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00612-2017"},{"key":"2024102102580032000_60.1.2102300.67","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02086-16"},{"key":"2024102102580032000_60.1.2102300.68","doi-asserted-by":"crossref","first-page":"2242","DOI":"10.1021\/acs.molpharmaceut.5b00852","article-title":"Primary air-liquid interface culture of nasal epithelium for nasal drug delivery","volume":"13","author":"Ong","year":"2016","journal-title":"Mol Pharm"},{"key":"2024102102580032000_60.1.2102300.69","doi-asserted-by":"crossref","first-page":"524","DOI":"10.3390\/diagnostics10080524","article-title":"Ciliary feature counter: a program for the quantitative assessment of cilia to diagnose primary ciliary dyskinesia","volume":"10","author":"Pinto","year":"2020","journal-title":"Diagnostics"}],"container-title":["European Respiratory Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1183\/13993003.02300-2021","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T07:07:00Z","timestamp":1740380820000},"score":1,"resource":{"primary":{"URL":"https:\/\/publications.ersnet.org\/lookup\/doi\/10.1183\/13993003.02300-2021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,20]]},"references-count":69,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,7,28]]},"published-print":{"date-parts":[[2022,7]]}},"alternative-id":["10.1183\/13993003.02300-2021"],"URL":"https:\/\/doi.org\/10.1183\/13993003.02300-2021","relation":{},"ISSN":["0903-1936","1399-3003"],"issn-type":[{"value":"0903-1936","type":"print"},{"value":"1399-3003","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,20]]}}}