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This study investigates the circulating proteomic profile associated with right heart maladaptive phenotype (RHMP) in PAH.<\/jats:p>\n                  <jats:p>Plasma proteomic profiling was performed using multiplex immunoassay in 121 (discovery cohort) and 76 (validation cohort) PAH patients. The association between proteomic markers and RHMP, defined by the Mayo right heart score (combining RV strain, New York Heart Association (NYHA) class and N-terminal pro-brain natriuretic peptide (NT-proBNP)) and Stanford score (RV end-systolic remodelling index, NYHA class and NT-proBNP), was assessed by partial least squares regression. Biomarker expression was measured in RV samples from PAH patients and controls, and pulmonary artery banding (PAB) mice.<\/jats:p>\n                  <jats:p>High levels of hepatocyte growth factor (HGF), stem cell growth factor-\u03b2, nerve growth factor and stromal derived factor-1 were associated with worse Mayo and Stanford scores independently from pulmonary resistance or pressure in both cohorts (the validation cohort had more severe disease features: lower cardiac index and higher NT-proBNP). In both cohorts, HGF added value to the REVEAL score in the prediction of death, transplant or hospitalisation at 3\u2005years. RV expression levels of HGF and its receptor c-Met were higher in end-stage PAH patients than controls, and in PAB mice than shams.<\/jats:p>\n                  <jats:p>High plasma HGF levels are associated with RHMP and predictive of 3-year clinical worsening. Both HGF and c-Met RV expression levels are increased in PAH. Assessing plasma HGF levels might identify patients at risk of heart failure who warrant closer follow-up and intensified therapy.<\/jats:p>","DOI":"10.1183\/13993003.02428-2020","type":"journal-article","created":{"date-parts":[[2020,12,22]],"date-time":"2020-12-22T18:22:58Z","timestamp":1608661378000},"page":"2002428","update-policy":"https:\/\/doi.org\/10.1183\/ers-crossmark-policy","source":"Crossref","is-referenced-by-count":32,"title":["Targeted proteomics of right heart adaptation to pulmonary arterial hypertension"],"prefix":"10.1183","volume":"57","author":[{"given":"Myriam","family":"Amsallem","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Sweatt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Arthur Ataam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Guihaire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Lecerf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e9lanie","family":"Lambert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5996-665X","authenticated-orcid":false,"given":"Maria Rosa","family":"Ghigna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Khadem","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqiang","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elie","family":"Fadel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlene","family":"Rabinovitch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinicio","family":"de Jesus Perez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edda","family":"Spiekerkoetter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olaf","family":"Mercier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francois","family":"Haddad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roham T.","family":"Zamanian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"81","published-online":{"date-parts":[[2020,12,17]]},"reference":[{"key":"2024102102042239000_57.4.2002428.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2016.10.047"},{"key":"2024102102042239000_57.4.2002428.2","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1016\/S2213-2600(17)30161-3","article-title":"Plasma proteome analysis in patients with pulmonary arterial hypertension: an observational cohort study","volume":"5","author":"Rhodes","year":"2017","journal-title":"Lancet Respir Med"},{"key":"2024102102042239000_57.4.2002428.3","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1161\/CIRCRESAHA.118.313911","article-title":"Discovery of distinct immune phenotypes using machine learning in pulmonary arterial hypertension","volume":"124","author":"Sweatt","year":"2019","journal-title":"Circ Res"},{"key":"2024102102042239000_57.4.2002428.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.hrtlng.2014.04.016"},{"key":"2024102102042239000_57.4.2002428.5","doi-asserted-by":"crossref","first-page":"609","DOI":"10.3389\/fphys.2018.00609","article-title":"Inflammatory mediators drive adverse right ventricular remodeling and dysfunction and serve as potential biomarkers","volume":"9","author":"Sydykov","year":"2018","journal-title":"Front Physiol"},{"key":"2024102102042239000_57.4.2002428.6","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCIMAGING.113.000640"},{"key":"2024102102042239000_57.4.2002428.7","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCIMAGING.116.005771"},{"key":"2024102102042239000_57.4.2002428.8","doi-asserted-by":"crossref","first-page":"26996","DOI":"10.1038\/srep26996","article-title":"The statistical value of raw fluorescence signal in Luminex xMAP based multiplex immunoassays","volume":"6","author":"Breen","year":"2016","journal-title":"Sci Rep"},{"key":"2024102102042239000_57.4.2002428.9","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.109.898122"},{"key":"2024102102042239000_57.4.2002428.10","doi-asserted-by":"crossref","unstructured":"National Research Council . 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