{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T19:17:51Z","timestamp":1762543071444,"version":"3.37.3"},"reference-count":32,"publisher":"European Respiratory Society (ERS)","issue":"2","license":[{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000098","name":"NIH Clinical Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000098","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["publications.ersnet.org"],"crossmark-restriction":true},"short-container-title":["ERJ Open Res"],"accepted":{"date-parts":[[2019,4,15]]},"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:p><jats:italic>Mycobacterium abscessus<\/jats:italic>is associated with antibiotic resistance and poor treatment outcomes. We described within-patient changes in<jats:italic>M. abscessus<\/jats:italic>resistance to clarithromycin and amikacin.<\/jats:p><jats:p>Patients with amikacin exposure and a &gt;50-month interval between<jats:italic>M. abscessus<\/jats:italic>isolates were identified. Antimicrobial susceptibility testing was performed on the first and last isolates by broth microdilution, and genetic markers of resistance were identified.<\/jats:p><jats:p>16 patients were identified with a median amikacin exposure of 2.3\u2005years (range 0.6\u20138.6\u2005years). 15 patients also received macrolides (median 7.2\u2005years, range 1.3\u201310.7\u2005years). All initial isolates were resistant to clarithromycin (minimum inhibitory concentration (MIC) \u22658\u2005\u00b5g\u00b7mL<jats:sup>\u22121<\/jats:sup>). Two patients had later susceptible isolates, which were of a different subspecies (<jats:italic>M. abscessus<\/jats:italic>subsp.<jats:italic>massiliense<\/jats:italic>) than the initial isolates (<jats:italic>M. abscessus<\/jats:italic>subsp.<jats:italic>abscessus<\/jats:italic>). All initial isolates were susceptible or intermediately resistant to amikacin, and only one patient had a resistant final isolate (MIC &gt;64\u2005\u00b5g\u00b7mL<jats:sup>\u22121<\/jats:sup>), accompanied by an A\u2192G mutation at position 1408 of the 16S ribosomal RNA. Forced expiratory volume in 1\u2005s decreased significantly over the study period, while smear quantity and the proportions of patients with elevated C-reactive protein or cavitary lesions all increased significantly.<\/jats:p><jats:p>Despite prolonged, mostly inhaled amikacin exposure, development of amikacin resistance was uncommon in this patient population; however, disease progression continued.<\/jats:p>","DOI":"10.1183\/23120541.00154-2018","type":"journal-article","created":{"date-parts":[[2019,5,28]],"date-time":"2019-05-28T14:59:13Z","timestamp":1559055553000},"page":"00154-2018","update-policy":"https:\/\/doi.org\/10.1183\/ers-crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Amikacin exposure and susceptibility of macrolide-resistant<i>Mycobacterium abscessus<\/i>"],"prefix":"10.1183","volume":"5","author":[{"given":"Shelby","family":"Daniel-Wayman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shamira","family":"Shallom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nabila","family":"Azeem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth\u00a0N.","family":"Olivier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian M.","family":"Zelazny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. 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