{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:35:52Z","timestamp":1790645752546,"version":"4.1.0"},"reference-count":28,"publisher":"European Respiratory Society (ERS)","issue":"1","license":[{"start":{"date-parts":[[2020,11,26]],"date-time":"2020-11-26T00:00:00Z","timestamp":1606348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001826","name":"ZonMw","doi-asserted-by":"publisher","award":["842002001"],"award-info":[{"award-number":["842002001"]}],"id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["publications.ersnet.org"],"crossmark-restriction":true},"short-container-title":["ERJ Open Res"],"accepted":{"date-parts":[[2020,11,6]]},"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>Several studies demonstrated that<jats:italic>Propionibacterium acnes<\/jats:italic>may be involved in sarcoidosis pathogenesis. Presence of<jats:italic>P. acnes<\/jats:italic>was found in granulomas of the majority of Japanese sarcoidosis patients. However, presence of<jats:italic>P. acnes<\/jats:italic>in tissue has never been related to sarcoidosis phenotypes and clinical outcome. Therefore, the aims of our study were to demonstrate whether<jats:italic>P. acnes<\/jats:italic>can be detected in granulomas of Dutch sarcoidosis patients and to investigate whether its presence is related to a clinical phenotype and\/or course of disease.<\/jats:p><jats:p>Sections of formalin-fixed paraffin-embedded tissue blocks of 76 sarcoidosis patients were examined by immunostaining with a<jats:italic>P. acnes-<\/jats:italic>specific monoclonal antibody (PAB antibody) using a Ventana BenchMark ULTRA. Clinical outcome status (COS) was determined and classified into two phenotype groups: A: resolved, minimal or persistent disease without treatment (COS 1\u20136) and B: persistent disease with need for treatment (COS 7\u20139).<\/jats:p><jats:p><jats:italic>P. acnes<\/jats:italic>was detected in samples of 31 patients (41%) and located within granulomas in samples of 13 patients (17%). The frequency of<jats:italic>P. acnes<\/jats:italic>detected in granulomas at diagnosis was significantly higher in patients with phenotype B compared to patients with phenotype A (29%<jats:italic>versus<\/jats:italic>0%, p=0.021).<\/jats:p><jats:p>Presence of<jats:italic>P. acnes<\/jats:italic>in granulomas can be confirmed in Dutch sarcoidosis patients. It is intriguing that presence of<jats:italic>P. acnes<\/jats:italic>in granulomas is more frequently found in patients with chronic disease requiring treatment. This adds to the rationale that a subgroup of sarcoidosis patients might benefit from antibiotic therapy.<\/jats:p>","DOI":"10.1183\/23120541.00486-2020","type":"journal-article","created":{"date-parts":[[2020,11,26]],"date-time":"2020-11-26T23:00:19Z","timestamp":1606431619000},"page":"00486-2020","update-policy":"https:\/\/doi.org\/10.1183\/ers-crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["Presence of<i>Propionibacterium acnes<\/i>in granulomas associates with a chronic disease course in Dutch sarcoidosis 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