{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T04:16:45Z","timestamp":1785298605542,"version":"3.55.0"},"reference-count":29,"publisher":"European Respiratory Society (ERS)","issue":"4","license":[{"start":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T00:00:00Z","timestamp":1740614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005925","name":"Buddhist Tzu Chi Medical Foundation","doi-asserted-by":"publisher","award":["TCMF-CP 112-02"],"award-info":[{"award-number":["TCMF-CP 112-02"]}],"id":[{"id":"10.13039\/501100005925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008108","name":"Taipei Tzu Chi Hospital","doi-asserted-by":"publisher","award":["TCRD-TPE-112-03"],"award-info":[{"award-number":["TCRD-TPE-112-03"]}],"id":[{"id":"10.13039\/501100008108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008108","name":"Taipei Tzu Chi Hospital","doi-asserted-by":"publisher","award":["TCRD-TPE-113-03"],"award-info":[{"award-number":["TCRD-TPE-113-03"]}],"id":[{"id":"10.13039\/501100008108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["publications.ersnet.org"],"crossmark-restriction":true},"short-container-title":["ERJ Open Res"],"accepted":{"date-parts":[[2025,1,31]]},"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Aspiration pneumonia is a critical concern, particularly in older adults with comorbidities. Micro-aspiration is often underdiagnosed due to the lack of precise diagnostic tools. This study evaluated the utility of anaerobic PCR for <jats:italic>Veillonella parvula<\/jats:italic> compared with traditional diagnostic methods for predicting aspiration pneumonia.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>This prospective observational case\u2013control study included patients with new-onset acute respiratory failure requiring mechanical ventilation in intensive care units (ICUs) at a Taiwan medical centre (2022\u20132024). Demographic data, laboratory results and tracheal aspirates were collected within 48\u2005h of ICU admission. PCR results for <jats:italic>Veillonella<\/jats:italic> spp. and <jats:italic>V. parvula<\/jats:italic>, along with pepsin and \u03b1-amylase levels, were analysed. Pneumonia diagnosis was confirmed by two pulmonologists.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Among 87 patients (53 with aspiration pneumonia, 34 without), aspiration pneumonia primarily affected the right side and had atypical phenotypes. Demographics, traditional risk factors, comorbidities and disease severity were similar between groups. <jats:italic>Veillonella<\/jats:italic> spp<jats:italic>.<\/jats:italic> PCR showed higher sensitivity (92.5%) and specificity (97.1%) than <jats:italic>V. parvula<\/jats:italic> PCR (79.2% and 97.1%), chest radiographs with symptoms (73.6% and 88.2%), \u03b1-amylase (28.3% and 64.7%) and pepsin (73.6% and 38.2%). Logistic regression revealed that <jats:italic>Veillonella<\/jats:italic> spp<jats:italic>.<\/jats:italic> PCR had an adjusted odds ratio of 467.2 (95% CI 30.96\u20137049.4, p&lt;0.001) for predicting aspiration pneumonia. Patients with aspiration pneumonia had longer ICU stays (18.28 days <jats:italic>versus<\/jats:italic> 13.03 days, p=0.001) and ventilator days (17.30 days <jats:italic>versus<\/jats:italic> 12.09 days, p=0.002).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>\n                     <jats:italic>Veillonella<\/jats:italic> spp. PCR offers better diagnostic sensitivity and specificity than traditional methods for aspiration pneumonia, potentially guiding antibiotic stewardship.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1183\/23120541.01286-2024","type":"journal-article","created":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T07:10:14Z","timestamp":1741331414000},"page":"01286-2024","update-policy":"https:\/\/doi.org\/10.1183\/ers-crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Utility of <i>Veillonella parvula<\/i> polymerase chain reaction in diagnosing aspiration pneumonia: a prospective observational study"],"prefix":"10.1183","volume":"11","author":[{"given":"Yao-Kuang","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6503-5189","authenticated-orcid":false,"given":"Mei-Chen","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4420-5756","authenticated-orcid":false,"given":"Hsueh-Wen","family":"Chung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chou-Chin","family":"Lan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lun-Yu","family":"Jao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia-Hui","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I-Shiang","family":"Tzeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Yu","family":"Chan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Ching","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You-Chen","family":"Chao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9065-2058","authenticated-orcid":false,"given":"Wen-Lin","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"81","published-online":{"date-parts":[[2025,2,27]]},"reference":[{"key":"2025081102300943000_11.4.01286-2024.1","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1186\/s12890-024-03015-8","article-title":"Incidence and characteristics of aspiration pneumonia in the Nagasaki Prefecture from 2005 to 2019","volume":"24","author":"Tomonaga","year":"2024","journal-title":"BMC Pulm Med"},{"key":"2025081102300943000_11.4.01286-2024.2","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1007\/s00455-022-10412-w","article-title":"Mortality from aspiration pneumonia: incidence, trends, and risk factors","volume":"37","author":"Gupte","year":"2022","journal-title":"Dysphagia"},{"key":"2025081102300943000_11.4.01286-2024.3","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200103013440908"},{"key":"2025081102300943000_11.4.01286-2024.4","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afp100"},{"key":"2025081102300943000_11.4.01286-2024.5","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1016\/j.amjmed.2013.07.012","article-title":"Risk factors for aspiration in community-acquired pneumonia: analysis of a hospitalized UK cohort","volume":"126","author":"Taylor","year":"2013","journal-title":"Am J Med"},{"key":"2025081102300943000_11.4.01286-2024.6","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-017-4736-x"},{"key":"2025081102300943000_11.4.01286-2024.7","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201104-0630OC"},{"key":"2025081102300943000_11.4.01286-2024.8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090851"},{"key":"2025081102300943000_11.4.01286-2024.9","doi-asserted-by":"crossref","first-page":"25","DOI":"10.3389\/fcimb.2015.00025","article-title":"Transcriptomic analysis of three Veillonella spp. present in carious dentine and in the saliva of caries-free individuals","volume":"5","author":"Do","year":"2015","journal-title":"Front Cell Infect Microbiol"},{"key":"2025081102300943000_11.4.01286-2024.10","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1001\/jamapediatrics.2014.696","article-title":"Changes in gastric and lung microflora with acid suppression: acid suppression and bacterial growth","volume":"168","author":"Rosen","year":"2014","journal-title":"JAMA Pediatr"},{"key":"2025081102300943000_11.4.01286-2024.11","doi-asserted-by":"crossref","first-page":"102667","DOI":"10.1016\/j.anaerobe.2022.102667","article-title":"Antibiotic susceptibility and biofilm-forming ability of Veillonella strains","volume":"78","author":"Li","year":"2022","journal-title":"Anaerobe"},{"key":"2025081102300943000_11.4.01286-2024.12","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/dst037"},{"key":"2025081102300943000_11.4.01286-2024.13","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e31820a856b"},{"key":"2025081102300943000_11.4.01286-2024.14","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1714562"},{"key":"2025081102300943000_11.4.01286-2024.15","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9681(87)90171-8"},{"key":"2025081102300943000_11.4.01286-2024.16","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-198608000-00028"},{"key":"2025081102300943000_11.4.01286-2024.17","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.58.5.377"},{"key":"2025081102300943000_11.4.01286-2024.18","doi-asserted-by":"publisher","DOI":"10.1111\/j.1447-0594.2012.00940.x"},{"key":"2025081102300943000_11.4.01286-2024.19","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00582-2017"},{"key":"2025081102300943000_11.4.01286-2024.20","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.jmii.2018.11.004","article-title":"Recommendations and guidelines for the treatment of pneumonia in Taiwan","volume":"52","author":"Chou","year":"2019","journal-title":"J Microbiol Immunol Infection"},{"key":"2025081102300943000_11.4.01286-2024.21","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000206106.65220.59"},{"key":"2025081102300943000_11.4.01286-2024.22","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-010-1892-7"},{"key":"2025081102300943000_11.4.01286-2024.23","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-014-3564-5"},{"key":"2025081102300943000_11.4.01286-2024.24","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s13613-020-00794-1","article-title":"Is COPD associated with increased risk for microaspiration in intubated critically ill patients?","volume":"11","author":"Degroote","year":"2021","journal-title":"Ann Intensive Care"},{"key":"2025081102300943000_11.4.01286-2024.25","doi-asserted-by":"crossref","first-page":"895537","DOI":"10.3389\/fmicb.2022.895537","article-title":"The oral microbiota: community composition, influencing factors, pathogenesis, and interventions","volume":"13","author":"Li","year":"2022","journal-title":"Front Microbiol"},{"key":"2025081102300943000_11.4.01286-2024.26","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.9.2908-2913.2002"},{"key":"2025081102300943000_11.4.01286-2024.27","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01215"},{"key":"2025081102300943000_11.4.01286-2024.28","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00461-20"},{"key":"2025081102300943000_11.4.01286-2024.29","doi-asserted-by":"crossref","first-page":"102611","DOI":"10.1016\/j.anaerobe.2022.102611","article-title":"Development of multiplex PCR panel for detection of anaerobic bacteria in clinical samples","volume":"76","author":"Olcu","year":"2022","journal-title":"Anaerobe"}],"container-title":["ERJ Open Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1183\/23120541.01286-2024","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T09:30:31Z","timestamp":1754904631000},"score":1,"resource":{"primary":{"URL":"https:\/\/publications.ersnet.org\/lookup\/doi\/10.1183\/23120541.01286-2024"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,27]]},"references-count":29,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,8,11]]},"published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1183\/23120541.01286-2024"],"URL":"https:\/\/doi.org\/10.1183\/23120541.01286-2024","relation":{},"ISSN":["2312-0541"],"issn-type":[{"value":"2312-0541","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,27]]}}}