{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T15:34:33Z","timestamp":1772897673049,"version":"3.50.1"},"reference-count":0,"publisher":"European Respiratory Society (ERS)","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur Respir J"],"published-print":{"date-parts":[[1997,8,1]]},"abstract":"<jats:p>We determined if alveolar macrophages (AMs) from infants with severe recurrent wheezing episodes release increased amounts of tumour necrosis factor-alpha (TNF-alpha), as described in adults with asthma. We compared TNF-alpha release by unstimulated and lipopolysaccharide-stimulated AMs obtained by bronchoalveolar lavage in 13 wheezy and seven nonwheezy infants (aged 6-36 months) and analysed its regulation by dexamethasone. Metabolites in cell supernatants were quantified by enzyme-linked immunosorbent assay (ELISA) (TNF-alpha) or radioimmunoassay (thromboxane B2 and prostaglandin E2). Comparison of results was performed by the Mann-Whitney U-test and values were expressed as median (interquartile range) in ng x 10(6) cells(-1). Resting AMs from wheezy infants released larger amounts of TNF-alpha and thromboxane B2 as compared to controls: 2.67 (0.89-8.33) vs 0.48 (0.25-1.08) and 75.63 (38.07-158.91) vs 10.03 (7.36-76.08), respectively (p&lt;0.05). When stimulated overnight with bacterial lipopolysaccharide, AMs from both groups released similar amounts of metabolites. Dexamethasone induced a consistent inhibition of the lipopolysaccharide-stimulated release of all the mediators. Our results show that alveolar macrophages from wheezy infants are activated to release increased amounts of tumour necrosis factor-alpha, as in asthma, and suggest that infants with recurrent wheezing may eventually benefit from treatment with glucocorticoids.<\/jats:p>","DOI":"10.1183\/09031936.97.10081767","type":"journal-article","created":{"date-parts":[[2003,2,6]],"date-time":"2003-02-06T23:58:35Z","timestamp":1044575915000},"page":"1767-1773","source":"Crossref","is-referenced-by-count":42,"title":["Increased spontaneous release of tumour necrosis factor-alpha by alveolar macrophages from wheezy infants"],"prefix":"10.1183","volume":"10","author":[{"given":"I","family":"Azevedo","sequence":"first","affiliation":[]},{"given":"J","family":"de Blic","sequence":"additional","affiliation":[]},{"given":"CH","family":"Dumarey","sequence":"additional","affiliation":[]},{"given":"P","family":"Scheinmann","sequence":"additional","affiliation":[]},{"given":"BB","family":"Vargaftig","sequence":"additional","affiliation":[]},{"given":"M","family":"Bachelet","sequence":"additional","affiliation":[]}],"member":"81","published-online":{"date-parts":[[1997,8,1]]},"container-title":["European Respiratory Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1183\/09031936.97.10081767","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T07:17:15Z","timestamp":1740381435000},"score":1,"resource":{"primary":{"URL":"https:\/\/publications.ersnet.org\/lookup\/doi\/10.1183\/09031936.97.10081767"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,8,1]]},"references-count":0,"journal-issue":{"issue":"8","published-online":{"date-parts":[[1997,8,1]]},"published-print":{"date-parts":[[1997,8,1]]}},"alternative-id":["10.1183\/09031936.97.10081767"],"URL":"https:\/\/doi.org\/10.1183\/09031936.97.10081767","relation":{},"ISSN":["0903-1936","1399-3003"],"issn-type":[{"value":"0903-1936","type":"print"},{"value":"1399-3003","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,8,1]]}}}