{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T12:49:44Z","timestamp":1765370984059,"version":"3.46.0"},"reference-count":61,"publisher":"European Respiratory Society (ERS)","issue":"178","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["publications.ersnet.org"],"crossmark-restriction":true},"short-container-title":["Eur Respir Rev"],"accepted":{"date-parts":[[2025,8,30]]},"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Molecular profiling has become essential in the management of nonsmall cell lung cancer (NSCLC). While endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is a cornerstone in diagnosis, tissue scarcity may hinder comprehensive testing. Recent studies suggest that supernatant from EBUS-TBNA could serve as an alternative source for molecular analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A systematic review and meta-analysis were conducted according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines (PROSPERO identifier CRD42024600046). Studies from 2000 to 2024 evaluating the feasibility, molecular concordance and turnaround time of EBUS-TBNA supernatant for molecular profiling in NSCLC were included. Outcomes included DNA yield, detection of actionable mutations and agreement with tissue-based results.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Seven studies (n=506 patients) were included. Feasibility of molecular analysis using supernatant was high (87\u2013100%). DNA yields varied across studies. In pooled analysis, storage temperature and preservation solution had no significant effect, while individual studies reported lower yields with high-speed centrifugation. Concordance with tissue samples ranged from 83% to 100%, with Cohen's \u03ba 0.947 (95% CI 0.905\u20130.989), indicating an almost perfect agreement. Supernatant samples demonstrated faster turnaround times (reduction of 1\u20137.5 days).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>EBUS-TBNA supernatant is a feasible and accurate source for molecular testing in NSCLC, with high concordance and shorter turnaround times. Standardisation of protocols is required before broader implementation in clinical practice.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1183\/16000617.0127-2025","type":"journal-article","created":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T09:30:15Z","timestamp":1765359015000},"page":"250127","update-policy":"https:\/\/doi.org\/10.1183\/ers-crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Supernatant from endobronchial ultrasound-guided transbronchial needle aspiration samples for molecular profiling in NSCLC: a systematic review and meta-analysis"],"prefix":"10.1183","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5929-6078","authenticated-orcid":false,"given":"Lu\u00eds","family":"Vaz 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