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Currently, due to lack of open-source software, this technique is widely performed by visually counting the ciliary beatings per a given time-window. Our aim was to generate open-source, fast and intuitive software for evaluating CBF, validated in Portuguese PCD patients and healthy volunteers.<\/jats:p><jats:p>Nasal brushings collected from 17 adult healthy volunteers and 34 PCD-referred subjects were recorded using HVMA. Evaluation of CBF was compared by two different methodologies: the new semi-automated computer software CiliarMove and the manual observation method using slow-motion movies. Clinical history, nasal nitric oxide and transmission electron microscopy were performed for diagnosis of PCD in the patient group. Genetic analysis was performed in a subset (n=8) of suspected PCD patients.<\/jats:p><jats:p>The correlation coefficient between the two methods was R<jats:sup>2<\/jats:sup>=0.9895. The interval of CBF values obtained from the healthy control group (n=17) was 6.18\u20139.17\u2005Hz at 25\u00b0C. In the PCD-excluded group (n=16), CBF ranged from 6.84 to 10.93\u2005Hz and in the PCD group (n=18), CBF ranged from 0 to 14.30\u2005Hz.<\/jats:p><jats:p>We offer an automated open-source programme named CiliarMove, validated by the manual observation method in a healthy volunteer control group, a PCD-excluded group and a PCD-confirmed group. In our hands, comparisons between CBF intervals alone could discern between healthy and PCD groups in 78% of the cases.<\/jats:p>","DOI":"10.1183\/23120541.00792-2020","type":"journal-article","created":{"date-parts":[[2021,2,8]],"date-time":"2021-02-08T22:06:25Z","timestamp":1612821985000},"page":"00792-2020","update-policy":"https:\/\/doi.org\/10.1183\/ers-crossmark-policy","source":"Crossref","is-referenced-by-count":26,"title":["CiliarMove: new software for evaluating ciliary beat frequency helps find novel mutations by a Portuguese multidisciplinary team on primary ciliary dyskinesia"],"prefix":"10.1183","volume":"7","author":[{"given":"Pedro","family":"Sampaio","sequence":"first","affiliation":[]},{"given":"M\u00f3nica Ferro","family":"da Silva","sequence":"additional","affiliation":[]},{"given":"In\u00eas","family":"Vale","sequence":"additional","affiliation":[]},{"given":"M\u00f3nica","family":"Roxo-Rosa","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0840-6844","authenticated-orcid":false,"given":"Andreia","family":"Pinto","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6596-1596","authenticated-orcid":false,"given":"Carolina","family":"Constant","sequence":"additional","affiliation":[]},{"given":"Luisa","family":"Pereira","sequence":"additional","affiliation":[]},{"given":"Carla M.","family":"Quint\u00e3o","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6733-6356","authenticated-orcid":false,"given":"Susana S.","family":"Lopes","sequence":"additional","affiliation":[]}],"member":"81","published-online":{"date-parts":[[2021,2,8]]},"reference":[{"key":"2024101708335176000_7.1.00792-2020.1","doi-asserted-by":"publisher","DOI":"10.1183\/16000617.0023-2017"},{"key":"2024101708335176000_7.1.00792-2020.2","doi-asserted-by":"publisher","DOI":"10.1136\/adc.2009.158493"},{"key":"2024101708335176000_7.1.00792-2020.3","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.97.10102376"},{"key":"2024101708335176000_7.1.00792-2020.4","doi-asserted-by":"publisher","DOI":"10.1136\/archdischild-2013-304831"},{"key":"2024101708335176000_7.1.00792-2020.5","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1002\/ppul.23304","article-title":"Diagnosis, monitoring, and treatment of primary ciliary dyskinesia: PCD foundation consensus recommendations based on state of the art review","volume":"51","author":"Shapiro","year":"2016","journal-title":"Pediatr Pulmonol"},{"key":"2024101708335176000_7.1.00792-2020.6","doi-asserted-by":"crossref","first-page":"2029","DOI":"10.1007\/s00018-019-03389-7","article-title":"Sperm defects in primary ciliary dyskinesia and related causes of male infertility","volume":"77","author":"Sironen","year":"2020","journal-title":"Cell Mol Life Sci"},{"key":"2024101708335176000_7.1.00792-2020.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.prrv.2015.09.001"},{"key":"2024101708335176000_7.1.00792-2020.8","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genom.7.080505.115610"},{"key":"2024101708335176000_7.1.00792-2020.9","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00046209"},{"key":"2024101708335176000_7.1.00792-2020.10","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.241760"},{"key":"2024101708335176000_7.1.00792-2020.11","doi-asserted-by":"crossref","unstructured":"Porter ME . 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