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Currently, two types of scrapie disease are known - classical and atypical\/Nor98 scrapie. In the present study we examine 24 cases of classical and 25 cases of atypical\/Nor98 scrapie with the sensitive PET blot method and validate the results with conventional immunohistochemistry. The sequential detection of PrP<jats:sup>Sc<\/jats:sup> aggregates in the CNS of classical scrapie sheep implies that after neuroinvasion a spread from spinal cord and obex to the cerebellum, diencephalon and frontal cortex via the rostral brainstem takes place. We categorize the spread of PrP<jats:sup>Sc<\/jats:sup> into four stages: the CNS entry stage, the brainstem stage, the cruciate sulcus stage and finally the basal ganglia stage. Such a sequential development of PrP<jats:sup>Sc<\/jats:sup> was not detectable upon analysis of the present atypical\/Nor98 scrapie cases. PrP<jats:sup>Sc<\/jats:sup> distribution in one case of atypical\/Nor98 scrapie in a presumably early disease phase suggests that the spread of PrP<jats:sup>Sc<\/jats:sup> aggregates starts in the di- or telencephalon. In addition to the spontaneous generation of PrP<jats:sup>Sc<\/jats:sup>, an uptake of the infectious agent into the brain, that bypasses the brainstem and starts its accumulation in the thalamus, needs to be taken into consideration for atypical\/Nor98 scrapie.<\/jats:p>","DOI":"10.1186\/1297-9716-42-32","type":"journal-article","created":{"date-parts":[[2011,2,15]],"date-time":"2011-02-15T23:37:30Z","timestamp":1297813050000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["PrPSc spreading patterns in the brain of sheep linked to different prion types"],"prefix":"10.1186","volume":"42","author":[{"given":"Wiebke M","family":"Wemheuer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie L","family":"Benestad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arne","family":"Wrede","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilhelm E","family":"Wemheuer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertram","family":"Brenig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bj\u00f8rn","family":"Bratberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter J","family":"Schulz-Schaeffer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2011,2,15]]},"reference":[{"key":"32_CR1","first-page":"365","volume":"5","author":"JP McGowan","year":"1922","unstructured":"McGowan JP: Scrapie in sheep. 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