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In the present study, detailed phenotypical and functional analyses of these T-helper cell subpopulations were performed. Na\u00efve CD8\u03b1<jats:sup>-<\/jats:sup>CD27<jats:sup>+<\/jats:sup> T helper cells predominantly resided in various lymph nodes, whereas higher proportions of CD8\u03b1<jats:sup>+<\/jats:sup>CD27<jats:sup>+<\/jats:sup> and CD8\u03b1<jats:sup>+<\/jats:sup>CD27<jats:sup>-<\/jats:sup> T helper cells were found in blood, spleen and liver. Both CD8\u03b1<jats:sup>+<\/jats:sup>CD27<jats:sup>+<\/jats:sup> and CD8\u03b1<jats:sup>+<\/jats:sup>CD27<jats:sup>-<\/jats:sup> T helper cells were capable of producing IFN-\u03b3 upon in vitro polyclonal stimulation and antigen-specific restimulation. Experiments with sorted CD8\u03b1<jats:sup>-<\/jats:sup>CD27<jats:sup>+<\/jats:sup>, CD8\u03b1<jats:sup>+<\/jats:sup>CD27<jats:sup>+<\/jats:sup> and CD8\u03b1<jats:sup>+<\/jats:sup>CD27<jats:sup>-<\/jats:sup> T-helper cell subsets following polyclonal stimulation revealed the lowest proliferative response but the highest ability for IFN-\u03b3 and TNF-\u03b1 production in the CD8\u03b1<jats:sup>+<\/jats:sup>CD27<jats:sup>-<\/jats:sup> subset. Therefore, these cells resembled terminally differentiated effector memory cells as described in human. This was supported by analyses of CCR7 and CD62L expression. CD8\u03b1<jats:sup>+<\/jats:sup>CD27<jats:sup>-<\/jats:sup> T helper cells were mostly CCR7<jats:sup>-<\/jats:sup> and had considerably reduced CD62L mRNA levels. In contrast, expression of both homing-receptors was increased on CD8\u03b1<jats:sup>+<\/jats:sup>CD27<jats:sup>+<\/jats:sup> T helper cells, which also had a proliferation rate similar to na\u00efve CD8\u03b1<jats:sup>-<\/jats:sup>CD27<jats:sup>+<\/jats:sup> T helper cells and showed intermediate levels of cytokine production. Therefore, similar to human, CD8\u03b1<jats:sup>+<\/jats:sup>CD27<jats:sup>+<\/jats:sup> T helper cells displayed a phenotype and functional properties of central memory cells.<\/jats:p>","DOI":"10.1186\/1297-9716-44-18","type":"journal-article","created":{"date-parts":[[2013,3,11]],"date-time":"2013-03-11T19:14:18Z","timestamp":1363029258000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":74,"title":["CD27 expression discriminates porcine T helper cells with functionally distinct properties"],"prefix":"10.1186","volume":"44","author":[{"given":"Katharina","family":"Reutner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Leitner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"M\u00fcllebner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Ladinig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine E","family":"Essler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J Catharina","family":"Duvigneau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"Ritzmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Steinberger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armin","family":"Saalm\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilhelm","family":"Gerner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,3,11]]},"reference":[{"key":"224_CR1","doi-asserted-by":"publisher","first-page":"1297","DOI":"10.1002\/eji.1830170912","volume":"17","author":"A Saalm\u00fcller","year":"1987","unstructured":"Saalm\u00fcller A, Reddehase MJ, B\u00fchring HJ, Jonjic S, Koszinowski UH: Simultaneous expression of CD4 and CD8 antigens by a substantial proportion of resting porcine T lymphocytes. 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