{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:13:51Z","timestamp":1790835231044,"version":"4.1.0"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2005,12,1]],"date-time":"2005-12-01T00:00:00Z","timestamp":1133395200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Respir Res"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background and methods<\/jats:title>\n            <jats:p>Human metapneumovirus (hMPV) is a recently discovered respiratory virus associated with bronchiolitis, pneumonia, croup and exacerbations of asthma. Since respiratory viruses are frequently detected in patients with acute exacerbations of COPD (AE-COPD) it was our aim to investigate the frequency of hMPV detection in a prospective cohort of hospitalized patients with AE-COPD compared to patients with stable COPD and to smokers without by means of quantitative real-time RT-PCR.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We analysed nasal lavage and induced sputum of 130 patients with AE-COPD, 65 patients with stable COPD and 34 smokers without COPD. HMPV was detected in 3\/130 (2.3%) AE-COPD patients with a mean of 6.5 \u00d7 10<jats:sup>5<\/jats:sup> viral copies\/ml in nasal lavage and 1.88 \u00d7 10<jats:sup>5<\/jats:sup> viral copies\/ml in induced sputum. It was not found in patients with stable COPD or smokers without COPD.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>HMPV is only found in a very small number of patients with AE-COPD. However it should be considered as a further possible viral trigger of AE-COPD because asymptomatic carriage is unlikely.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1465-9921-6-150","type":"journal-article","created":{"date-parts":[[2005,12,21]],"date-time":"2005-12-21T19:14:14Z","timestamp":1135192454000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Relevance of human metapneumovirus in exacerbations of COPD"],"prefix":"10.1186","volume":"6","author":[{"given":"G","family":"Rohde","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Borg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U","family":"Arinir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Kronsbein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Rausse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TT","family":"Bauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Bufe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Schultze-Werninghaus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2005,12,21]]},"reference":[{"key":"388_CR1_388","doi-asserted-by":"publisher","first-page":"1618","DOI":"10.1164\/ajrccm.164.9.2105011","volume":"164","author":"T Seemungal","year":"2001","unstructured":"Seemungal T, Harper-Owen R, Bhowmik A, Moric I, Sanderson G, Message S, Maccallum P, Meade TW, Jeffries DJ, Johnston SL, Wedzicha JA: Respiratory Viruses, Symptoms, and Inflammatory Markers in Acute Exacerbations and Stable Chronic Obstructive Pulmonary Disease.\nAm J Respir Crit Care Med 2001, 164:1618\u20131623.","journal-title":"Am J Respir Crit Care Med"},{"key":"388_CR2_388","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1136\/thorax.58.1.37","volume":"58","author":"G Rohde","year":"2003","unstructured":"Rohde G, Wiethege A, Borg I, Kauth M, Bauer TT, Gillissen A, Bufe A, Schultze-Werninghaus G: Respiratory viruses in exacerbations of chronic obstructive pulmonary disease requiring hospitalization - a case-control study.\nThorax 2003, 58:37\u201342.","journal-title":"Thorax"},{"key":"388_CR3_388","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1038\/89098","volume":"7","author":"BG van den Hoogen","year":"2001","unstructured":"van den Hoogen BG, de Jong JC, Groen J, Kuiken T, de Groot R, Fouchier RA, Osterhaus AD: A newly discovered human pneumovirus isolated from young children with respiratory tract disease.\nNat Med 2001, 7:719\u2013724.","journal-title":"Nat Med"},{"key":"388_CR4_388","doi-asserted-by":"publisher","first-page":"1407","DOI":"10.1542\/peds.111.6.1407","volume":"111","author":"F Esper","year":"2003","unstructured":"Esper F, Boucher D, Weibel C, Martinello RA, Kahn JS: Human metapneumovirus infection in the United States: clinical manifestations associated with a newly emerging respiratory infection in children.\nPediatrics 2003, 111:1407\u20131410.","journal-title":"Pediatrics"},{"key":"388_CR5_388","doi-asserted-by":"publisher","first-page":"1626","DOI":"10.3201\/eid0912.030522","volume":"9","author":"LE Cuevas","year":"2003","unstructured":"Cuevas LE, Nasser AM, Dove W, Gurgel RQ, Greensill J, Hart CA: Human metapneumovirus and respiratory syncytial virus, Brazil.\nEmerg Infect Dis 2003, 9:1626\u20131628.","journal-title":"Emerg Infect Dis"},{"key":"388_CR6_388","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1128\/JCM.42.1.126-132.2004","volume":"42","author":"T Ebihara","year":"2004","unstructured":"Ebihara T, Endo R, Kikuta H, Ishiguro N, Ishiko H, Hara M, Takahashi Y, Kobayashi K: Human metapneumovirus infection in Japanese children.\nJ Clin Microbiol 2004, 42:126\u2013132.","journal-title":"J Clin Microbiol"},{"key":"388_CR7_388","doi-asserted-by":"crossref","first-page":"188","DOI":"10.5694\/j.1326-5377.2002.tb04354.x","volume":"176","author":"MD Nissen","year":"2002","unstructured":"Nissen MD, Siebert DJ, Mackay IM, Sloots TP, Withers SJ: Evidence of human metapneumovirus in Australian children.\nMed J Aust 2002, 176:188.","journal-title":"Med J Aust"},{"key":"388_CR8_388","doi-asserted-by":"publisher","first-page":"897","DOI":"10.3201\/eid0809.020084","volume":"8","author":"J Stockton","year":"2002","unstructured":"Stockton J, Stephenson I, Fleming D, Zambon M: Human metapneumovirus as a cause of community-acquired respiratory illness.\nEmerg Infect Dis 2002, 8:897\u2013901.","journal-title":"Emerg Infect Dis"},{"key":"388_CR9_388","doi-asserted-by":"publisher","first-page":"700","DOI":"10.3201\/eid1004.030555","volume":"10","author":"JA Mullins","year":"2004","unstructured":"Mullins JA, Erdman DD, Weinberg GA, Edwards K, Hall CB, Walker FJ, Iwane M, Anderson LJ: Human metapneumovirus infection among children hospitalized with acute respiratory illness.\nEmerg Infect Dis 2004, 10:700\u2013705.","journal-title":"Emerg Infect Dis"},{"key":"388_CR10_388","doi-asserted-by":"publisher","first-page":"1095","DOI":"10.3201\/eid1006.030629","volume":"10","author":"T Jartti","year":"2004","unstructured":"Jartti T, Lehtinen P, Vuorinen T, Osterback R, van den HB, Osterhaus AD, Ruuskanen O: Respiratory picornaviruses and respiratory syncytial virus as causative agents of acute expiratory wheezing in children.\nEmerg Infect Dis 2004, 10:1095\u20131101.","journal-title":"Emerg Infect Dis"},{"key":"388_CR11_388","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1002\/jmv.20116","volume":"73","author":"FF IJpma","year":"2004","unstructured":"IJpma FF, Beekhuis D, Cotton MF, Pieper CH, Kimpen JL, van den Hoogen BG, van Doornum GJ, Osterhaus DM: Human metapneumovirus infection in hospital referred South African children.\nJ Med Virol 2004, 73:486\u2013493.","journal-title":"J Med Virol"},{"key":"388_CR12_388","doi-asserted-by":"publisher","first-page":"1571","DOI":"10.1086\/379200","volume":"188","author":"BG van den Hoogen","year":"2003","unstructured":"van den Hoogen BG, van Doornum GJ, Fockens JC, Cornelissen JJ, Beyer WE, de Groot R, Osterhaus AD, Fouchier RA: Prevalence and clinical symptoms of human metapneumovirus infection in hospitalized patients.\nJ Infect Dis 2003, 188:1571\u20131577.","journal-title":"J Infect Dis"},{"key":"388_CR13_388","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1086\/421120","volume":"190","author":"AJ McAdam","year":"2004","unstructured":"McAdam AJ, Hasenbein ME, Feldman HA, Cole SE, Offermann JT, Riley AM, Lieu TA: Human metapneumovirus in children tested at a tertiary-care hospital.\nJ Infect Dis 2004, 190:20\u201326.","journal-title":"J Infect Dis"},{"key":"388_CR14_388","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1056\/NEJMoa025472","volume":"350","author":"JV Williams","year":"2004","unstructured":"Williams JV, Harris PA, Tollefson SJ, Halburnt-Rush LL, Pingsterhaus JM, Edwards KM, Wright PF, Crowe JEJ: Human Metapneumovirus and Lower Respiratory Tract Disease in Otherwise Healthy Infants and Children.\nThe New England Journal of Medicine 2004, 350:443\u2013450.","journal-title":"The New England Journal of Medicine"},{"key":"388_CR15_388","doi-asserted-by":"publisher","first-page":"2047","DOI":"10.1086\/383350","volume":"189","author":"FR Laham","year":"2004","unstructured":"Laham FR, Israele V, Casellas JM, Garcia AM, Lac Prugent CM, Hoffman SJ, Hauer D, Thumar B, Name MI, Pascual A, Taratutto N, Ishida MT, Balduzzi M, Maccarone M, Jackli S, Passarino R, Gaivironsky RA, Karron RA, Polack NR, Polack FP: Differential production of inflammatory cytokines in primary infection with human metapneumovirus and with other common respiratory viruses of infancy.\nJ Infect Dis 2004, 189:2047\u20132056.","journal-title":"J Infect Dis"},{"key":"388_CR16_388","doi-asserted-by":"publisher","first-page":"4642","DOI":"10.1128\/JCM.41.10.4642-4646.2003","volume":"41","author":"N Bastien","year":"2003","unstructured":"Bastien N, Ward D, Van Caeseele P, Brandt K, Lee SH, McNabb G, Klisko B, Chan E, Li Y: Human metapneumovirus infection in the Canadian population.\nJ Clin Microbiol 2003, 41:4642\u20134646.","journal-title":"J Clin Microbiol"},{"key":"388_CR17_388","doi-asserted-by":"publisher","first-page":"785","DOI":"10.1086\/367901","volume":"187","author":"AR Falsey","year":"2003","unstructured":"Falsey AR, Erdman D, Anderson LJ, Walsh EE: Human metapneumovirus infections in young and elderly adults.\nJ Infect Dis 2003, 187:785\u2013790.","journal-title":"J Infect Dis"},{"key":"388_CR18_388","doi-asserted-by":"publisher","first-page":"497","DOI":"10.3201\/eid1003.030513","volume":"10","author":"PK Chan","year":"2004","unstructured":"Chan PK, To KF, Wu A, Tse GM, Chan KF, Lui SF, Sung JJ, Tam JS, Tomlinson B: Human metapneumovirus-associated atypical pneumonia and SARS.\nEmerg Infect Dis 2004, 10:497\u2013500.","journal-title":"Emerg Infect Dis"},{"key":"388_CR19_388","doi-asserted-by":"publisher","first-page":"1338","DOI":"10.3201\/eid1007.030633","volume":"10","author":"D Vicente","year":"2004","unstructured":"Vicente D, Montes M, Cilla G, Perez-Trallero E: Human metapneumovirus and chronic obstructive pulmonary disease.\nEmerg Infect Dis 2004, 10:1338\u20131339.","journal-title":"Emerg Infect Dis"},{"key":"388_CR20_388","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1159\/000080043","volume":"135","author":"G Rohde","year":"2004","unstructured":"Rohde G, Gevaert P, Holtappels G, Fransen L, Borg I, Wiethege A, Arinir U, Schultze-Wernighaus G, Bachert C: Soluble IL-5 receptor a is increased in acute exacerbation of COPD.\nInt Arch Allergy Immunol 2004, 135:54\u201361.","journal-title":"Int Arch Allergy Immunol"},{"key":"388_CR21_388","doi-asserted-by":"publisher","first-page":"1256","DOI":"10.1164\/ajrccm.163.5.2101039","volume":"163","author":"RA Pauwels","year":"2001","unstructured":"Pauwels RA, Buist AS, Calverley PM, Jenkins CR, Hurd SS: Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease. NHLBI\/WHO Global Initiative for Chronic Obstructive Lung Disease (GOLD) Workshop summary.\nAm J Respir Crit Care Med 2001, 163:1256\u20131276.","journal-title":"Am J Respir Crit Care Med"},{"key":"388_CR22_388","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1128\/JCM.42.3.981-986.2004","volume":"42","author":"J Maertzdorf","year":"2004","unstructured":"Maertzdorf J, Wang CK, Brown JB, Quinto JD, Chu M, de Graaf M, van den Hoogen BG, Spaete R, Osterhaus ADME, Fouchier RAM: Real-Time Reverse Transcriptase PCR Assay for Detection of Human Metapneumoviruses from All Known Genetic Lineages.\nJ Clin Microbiol 2004, 42:981\u2013986.","journal-title":"J Clin Microbiol"},{"key":"388_CR23_388","doi-asserted-by":"publisher","first-page":"944","DOI":"10.1183\/09031936.03.00088102","volume":"21","author":"I Borg","year":"2003","unstructured":"Borg I, Rohde G, Loeseke S, Bittscheid J, Schultze-Werninghaus G, Stephan V, Bufe A: Evaluation of a quantitative real-time PCR for the detection of RSV in pulmonary diseases.\nEur Respir J 2003, 21:944\u2013951.","journal-title":"Eur Respir J"},{"key":"388_CR24_388","volume-title":"Journal of Clinical Virology","author":"AR Falsey","year":"2005","unstructured":"Falsey AR, Criddle MC, Walsh EE: Detection of respiratory syncytial virus and human metapneumovirus by reverse transcription polymerase chain reaction in adults with and without respiratory illness.\nJournal of Clinical Virology 2005., [Epub ahead of print]:"},{"key":"388_CR25_388","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1086\/431981","volume":"41","author":"ME Hamelin","year":"2005","unstructured":"Hamelin ME, Cote S, Laforge J, Lampron N, Bourbeau J, Weiss K, Gilca R, DeSerres G, Boivin G: Human metapneumovirus infection in adults with community-acquired pneumonia and exacerbation of chronic obstructive pulmonary disease.\nClin Infect Dis 2005, 41:498\u2013502.","journal-title":"Clin Infect Dis"},{"key":"388_CR26_388","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1016\/j.jinf.2004.07.011","volume":"50","author":"JD Beckham","year":"2005","unstructured":"Beckham JD, Cadena A, Lin J, Piedra PA, Glezen WP, Greenberg SB, Atmar RL: Respiratory viral infections in patients with chronic, obstructive pulmonary disease.\nJ Infect 2005, 50:322\u2013330.","journal-title":"J Infect"},{"key":"388_CR27_388","doi-asserted-by":"publisher","first-page":"1653","DOI":"10.1378\/chest.118.6.1653","volume":"118","author":"GC Riise","year":"2000","unstructured":"Riise GC, Andersson R, Bergstrom T, Lundmark A, Nilsson FN, Olofsson S: Quantification of Cytomegalovirus DNA in BAL Fluid : A Longitudinal Study in Lung Transplant Recipients.\nChest 2000, 118:1653\u20131660.","journal-title":"Chest"},{"key":"388_CR28_388","doi-asserted-by":"publisher","first-page":"1550","DOI":"10.3201\/eid1009.040058","volume":"10","author":"IF Hung","year":"2004","unstructured":"Hung IF, Cheng VC, Wu AK, Tang BS, Chan KH, Chu CM, Wong MM, Hui WT, Poon LL, Tse DM, Chan KS, Woo PC, Lau SK, Peiris JS, Yuen KY: Viral loads in clinical specimens and SARS manifestations.\nEmerg Infect Dis 2004, 10:1550\u20131557.","journal-title":"Emerg Infect Dis"}],"container-title":["Respiratory Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/1465-9921-6-150.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/1465-9921-6-150\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/1465-9921-6-150","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1465-9921-6-150.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T01:16:46Z","timestamp":1630459006000},"score":1,"resource":{"primary":{"URL":"https:\/\/respiratory-research.biomedcentral.com\/articles\/10.1186\/1465-9921-6-150"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,12]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,12]]}},"alternative-id":["388"],"URL":"https:\/\/doi.org\/10.1186\/1465-9921-6-150","relation":{},"ISSN":["1465-993X"],"issn-type":[{"value":"1465-993X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,12]]},"assertion":[{"value":"11 November 2005","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 December 2005","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 December 2005","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"150"}}