{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T10:56:45Z","timestamp":1790506605563,"version":"4.1.0"},"reference-count":44,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2005,1,8]],"date-time":"2005-01-08T00:00:00Z","timestamp":1105142400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0\/"},{"start":{"date-parts":[[2005,1,8]],"date-time":"2005-01-08T00:00:00Z","timestamp":1105142400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0\/"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Respir Res"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                        <jats:title>Background<\/jats:title>\n                        <jats:p>It has recently been suggested that RhoA plays an important role in the enhancement of the Ca<jats:sup>2+<\/jats:sup> sensitization of smooth muscle contraction. In the present study, a participation of RhoA-mediated Ca<jats:sup>2+<\/jats:sup> sensitization in the augmented bronchial smooth muscle (BSM) contraction in a murine model of allergic asthma was examined.<\/jats:p>\n                     <\/jats:sec><jats:sec>\n                        <jats:title>Methods<\/jats:title>\n                        <jats:p>Ovalbumin (OA)-sensitized BALB\/c mice were repeatedly challenged with aerosolized OA and sacrificed 24 hours after the last antigen challenge. The contractility and RhoA protein expression of BSMs were measured by organ-bath technique and immunoblotting, respectively.<\/jats:p>\n                     <\/jats:sec><jats:sec>\n                        <jats:title>Results<\/jats:title>\n                        <jats:p>Repeated OA challenge to sensitized mice caused a BSM hyperresponsiveness to acetylcholine (ACh), but not to high K<jats:sup>+<\/jats:sup>-depolarization. In \u03b1-toxin-permeabilized BSMs, ACh induced a Ca<jats:sup>2+<\/jats:sup> sensitization of contraction, which is sensitive to <jats:italic>Clostridium botulinum<\/jats:italic> C3 exoenzyme, indicating that RhoA is implicated in this Ca<jats:sup>2+<\/jats:sup> sensitization. Interestingly, the ACh-induced, RhoA-mediated Ca<jats:sup>2+<\/jats:sup> sensitization was significantly augmented in permeabilized BSMs of OA-challenged mice. Moreover, protein expression of RhoA was significantly increased in the hyperresponsive BSMs.<\/jats:p>\n                     <\/jats:sec><jats:sec>\n                        <jats:title>Conclusion<\/jats:title>\n                        <jats:p>These findings suggest that the augmentation of Ca<jats:sup>2+<\/jats:sup> sensitizing effect, probably via an up-regulation of RhoA protein, might be involved in the enhanced BSM contraction in antigen-induced airway hyperresponsiveness.<\/jats:p>\n                     <\/jats:sec>","DOI":"10.1186\/1465-9921-6-4","type":"journal-article","created":{"date-parts":[[2005,1,12]],"date-time":"2005-01-12T22:25:51Z","timestamp":1105568751000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":80,"title":["Involvement of RhoA-mediated Ca2+ sensitization in antigen-induced bronchial smooth muscle hyperresponsiveness in mice"],"prefix":"10.1186","volume":"6","author":[{"given":"Yoshihiko","family":"Chiba","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayako","family":"Ueno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Shinozaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisao","family":"Takeyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuji","family":"Nakazawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyasu","family":"Sakai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miwa","family":"Misawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2005,1,8]]},"reference":[{"key":"242_CR1_242","first-page":"389","volume":"121","author":"HA Boushey","year":"1980","unstructured":"Boushey HA, Holtzman MJ, Sheller JR, Nadel JA: Bronchial hyperreactivity.\n                           Am Rev Respir Dis 1980, 121:389\u2013413.","journal-title":"Am Rev Respir Dis"},{"key":"242_CR2_242","first-page":"149","volume-title":"Airway secretion: physiological basis for the control of mucus hypersecretion","author":"PK Jeffery","year":"1993","unstructured":"Jeffery PK: Microscopic structure of airway secretory cells: variation in hypersecretory disease and effects of drugs. 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