{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T16:25:32Z","timestamp":1789748732941,"version":"4.0.1"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Biochem"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Mutations to the <jats:italic>TSC1<\/jats:italic> and <jats:italic>TSC2<\/jats:italic> genes cause the disease tuberous sclerosis complex. The <jats:italic>TSC1<\/jats:italic> and <jats:italic>TSC2<\/jats:italic> gene products form a protein complex that integrates multiple metabolic signals to regulate the activity of the target of rapamycin (TOR) complex 1 (TORC1) and thereby control cell growth. Here we investigate the quaternary structure of the TSC1-TSC2 complex by gel filtration and coimmunoprecipitation.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>TSC1 and TSC2 co-eluted in high molecular weight fractions by gel filtration. Coimmunoprecipitation of distinct tagged TSC1 and TSC2 isoforms demonstrated that TSC1-TSC2 complexes contain multiple TSC1 and TSC2 subunits.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>TSC1 and TSC2 interact to form large complexes containing multiple TSC1 and TSC2 subunits.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2091-13-18","type":"journal-article","created":{"date-parts":[[2012,9,24]],"date-time":"2012-09-24T23:09:34Z","timestamp":1348528174000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["The TSC1-TSC2 complex consists of multiple TSC1 and TSC2 subunits"],"prefix":"10.1186","volume":"13","author":[{"given":"Marianne","family":"Hoogeveen-Westerveld","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leontine","family":"van Unen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ans","family":"van den Ouweland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dicky","family":"Halley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre","family":"Hoogeveen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Nellist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,9,24]]},"reference":[{"key":"394_CR1","doi-asserted-by":"publisher","first-page":"805","DOI":"10.1126\/science.277.5327.805","volume":"277","author":"M van Slegtenhorst","year":"1997","unstructured":"van Slegtenhorst M, de Hoogt R, Hermans C, Nellist M, Janssen LAJ, Verhoef S, Lindhout D, van den Ouweland AMW, Halley DJJ, Young J, Burley M, Jeremiah S, Woodward K, Nahmias J, Fox M, Ekong R, Wolfe J, Povey S, Osborne J, Snell RG, Cheadle JP, Jones AC, Tachataki M, Ravine D, Sampson JR, Reeve MP, Richardson P, Wilmer F, Munro C, Hawkins TL, Sepp T, Ali JBM, Ward S, Green AJ, Yates JRW, Short MP, Haines JH, Jozwiak S, Kwiatkowska J, Henske EP, Kwiatkowski DJ: Identification of the tuberous sclerosis gene TSC1 on chromosome 9q34. 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