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Due to the impracticality of experimental approaches to systematically scan the entire protein, a computational tool that predicts the most probable epitope regions is desirable.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The Epitopia server is a web-based tool that aims to predict immunogenic regions in either a protein three-dimensional structure or a linear sequence. Epitopia implements a machine-learning algorithm that was trained to discern antigenic features within a given protein. The Epitopia algorithm has been compared to other available epitope prediction tools and was found to have higher predictive power. A special emphasis was put on the development of a user-friendly graphical interface for displaying the results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Epitopia is a user-friendly web-server that predicts immunogenic regions for both a protein structure and a protein sequence. Its accuracy and functionality make it a highly useful tool. Epitopia is available at<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/epitopia.tau.ac.il\" ext-link-type=\"uri\">http:\/\/epitopia.tau.ac.il<\/jats:ext-link>and includes extensive explanations and example predictions.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1471-2105-10-287","type":"journal-article","created":{"date-parts":[[2009,9,15]],"date-time":"2009-09-15T06:15:01Z","timestamp":1252995301000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":195,"title":["Epitopia: a web-server for predicting B-cell epitopes"],"prefix":"10.1186","volume":"10","author":[{"given":"Nimrod D","family":"Rubinstein","sequence":"first","affiliation":[]},{"given":"Itay","family":"Mayrose","sequence":"additional","affiliation":[]},{"given":"Eric","family":"Martz","sequence":"additional","affiliation":[]},{"given":"Tal","family":"Pupko","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2009,9,14]]},"reference":[{"issue":"3","key":"3017_CR1","doi-asserted-by":"publisher","first-page":"314","DOI":"10.1016\/S1367-5931(00)00208-8","volume":"5","author":"MB Irving","year":"2001","unstructured":"Irving MB, Pan O, Scott JK: Random-peptide libraries and antigen-fragment libraries for epitope mapping and the development of vaccines and diagnostics. 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