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This is a major cause of death in the protected species in the Alps (e.g., <jats:italic>Capra ibex<\/jats:italic>, <jats:italic>Rupicapra rupicapra<\/jats:italic>).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>The genome was sequenced using a combined technique of GS-FLX (454) and Sanger sequencing, and annotated by an automatic pipeline that we designed using several tools interconnected via PERL scripts. The resulting annotations are stored in a MySQL database.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The annotated sequence is deposited in the EMBL database (FM864216) and uploaded into the mollicutes database MolliGen <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/cbi.labri.fr\/outils\/molligen\/\" ext-link-type=\"uri\">http:\/\/cbi.labri.fr\/outils\/molligen\/<\/jats:ext-link> allowing for comparative genomics.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>We show that our automatic pipeline allows for annotating a complete mycoplasma genome and present several examples of analysis in search for biological targets (e.g., pathogenic proteins).<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2105-10-s6-s7","type":"journal-article","created":{"date-parts":[[2009,6,16]],"date-time":"2009-06-16T18:15:51Z","timestamp":1245176151000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["The Mycoplasma conjunctivae genome sequencing, annotation and analysis"],"prefix":"10.1186","volume":"10","author":[{"given":"Sandra P","family":"Calderon-Copete","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Wigger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christof","family":"Wunderlin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Schmidheini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Frey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A","family":"Quail","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Falquet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2009,6,16]]},"reference":[{"issue":"4","key":"3302_CR1","doi-asserted-by":"publisher","first-page":"1093","DOI":"10.1099\/00207713-46-4-1093","volume":"46","author":"B Pettersson","year":"1996","unstructured":"Pettersson B, Uhl\u00e9n M, Johansson KE: Phylogeny of some mycoplasmas from ruminants based on 16S rRNA sequences and definition of a new cluster within the hominis group. 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