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The discretization can be exploited to efficiently search the conformational space. However, discretizing this naturally continuous space comes at the cost of losing detailed information that is crucial for certain applications. For example, rigorously combining rotamers with physical force fields is associated with numerous problems.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>In this work we present BASILISK: a generative, probabilistic model of the conformational space of side chains that makes it possible to sample in continuous space. In addition, sampling can be conditional upon the protein's detailed backbone conformation, again in continuous space - without involving discretization.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>A careful analysis of the model and a comparison with various rotamer libraries indicates that the model forms an excellent, fully continuous model of side chain conformational space. We also illustrate how the model can be used for rigorous, unbiased sampling with a physical force field, and how it improves side chain prediction when used as a pseudo-energy term. In conclusion, BASILISK is an important step forward on the way to a rigorous probabilistic description of protein structure in continuous space and in atomic detail.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2105-11-306","type":"journal-article","created":{"date-parts":[[2010,6,5]],"date-time":"2010-06-05T18:13:46Z","timestamp":1275761626000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Beyond rotamers: a generative, probabilistic model of side chains in proteins"],"prefix":"10.1186","volume":"11","author":[{"given":"Tim","family":"Harder","sequence":"first","affiliation":[]},{"given":"Wouter","family":"Boomsma","sequence":"additional","affiliation":[]},{"given":"Martin","family":"Paluszewski","sequence":"additional","affiliation":[]},{"given":"Jes","family":"Frellsen","sequence":"additional","affiliation":[]},{"given":"Kristoffer E","family":"Johansson","sequence":"additional","affiliation":[]},{"given":"Thomas","family":"Hamelryck","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2010,6,5]]},"reference":[{"key":"3763_CR1","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1111\/j.1399-3011.1970.tb01679.x","volume":"2","author":"R Chandrasekaran","year":"1970","unstructured":"Chandrasekaran R, Ramachandran GN: Studies on the conformation of amino acids. 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