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To benefit from the rapidly increasing structural data, it is essential to improve the tools that enable large scale binding site prediction with greater emphasis on their biological validity.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We have developed a new method for the annotation of protein-small molecule binding sites, using inference by homology, which allows us to extend annotation onto protein sequences without experimental data available. To ensure biological relevance of binding sites, our method clusters similar binding sites found in homologous protein structures based on their sequence and structure conservation. Binding sites which appear evolutionarily conserved among non-redundant sets of homologous proteins are given higher priority. After binding sites are clustered, position specific score matrices (PSSMs) are constructed from the corresponding binding site alignments. Together with other measures, the PSSMs are subsequently used to rank binding sites to assess how well they match the query and to better gauge their biological relevance. The method also facilitates a succinct and informative representation of observed and inferred binding sites from homologs with known three-dimensional structures, thereby providing the means to analyze conservation and diversity of binding modes. Furthermore, the chemical properties of small molecules bound to the inferred binding sites can be used as a starting point in small molecule virtual screening. The method was validated by comparison to other binding site prediction methods and to a collection of manually curated binding site annotations. We show that our method achieves a sensitivity of 72% at predicting biologically relevant binding sites and can accurately discriminate those sites that bind biological small molecules from non-biological ones.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>A new algorithm has been developed to predict binding sites with high accuracy in terms of their biological validity. It also provides a common platform for function prediction, knowledge-based docking and for small molecule virtual screening. The method can be applied even for a query sequence without structure. The method is available at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.ncbi.nlm.nih.gov\/Structure\/ibis\/ibis.cgi\" ext-link-type=\"uri\">http:\/\/www.ncbi.nlm.nih.gov\/Structure\/ibis\/ibis.cgi<\/jats:ext-link>.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2105-11-365","type":"journal-article","created":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T18:15:41Z","timestamp":1278008141000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Knowledge-based annotation of small molecule binding sites in proteins"],"prefix":"10.1186","volume":"11","author":[{"given":"Ratna R","family":"Thangudu","sequence":"first","affiliation":[]},{"given":"Manoj","family":"Tyagi","sequence":"additional","affiliation":[]},{"given":"Benjamin A","family":"Shoemaker","sequence":"additional","affiliation":[]},{"given":"Stephen H","family":"Bryant","sequence":"additional","affiliation":[]},{"given":"Anna R","family":"Panchenko","sequence":"additional","affiliation":[]},{"given":"Thomas","family":"Madej","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2010,7,1]]},"reference":[{"key":"3822_CR1","first-page":"D298","volume-title":"Nucleic Acids Res","author":"Y Wang","year":"2007","unstructured":"Wang Y, Addess KJ, Chen J, Geer LY, He J, He S, Lu S, Madej T, Marchler-Bauer A, Thiessen PA, et al.: MMDB: annotating protein sequences with Entrez's 3D-structure database. 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