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To study how events in transcription elongation affect translation elongation and fluctuations in protein levels, we propose a delayed stochastic model of prokaryotic transcription and translation at the nucleotide and codon level that includes the promoter open complex formation and alternative pathways to elongation, namely pausing, arrests, editing, pyrophosphorolysis, RNA polymerase traffic, and premature termination. Stepwise translation can start after the ribosome binding site is formed and accounts for variable codon translation rates, ribosome traffic, back-translocation, drop-off, and trans-translation.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>First, we show that the model accurately matches measurements of sequence-dependent translation elongation dynamics. Next, we characterize the degree of coupling between fluctuations in RNA and protein levels, and its dependence on the rates of transcription and translation initiation. Finally, modeling sequence-specific transcriptional pauses, we find that these affect protein noise levels.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>For parameter values within realistic intervals, transcription and translation are found to be tightly coupled in <jats:italic>Escherichia coli<\/jats:italic>, as the noise in protein levels is mostly determined by the underlying noise in RNA levels. Sequence-dependent events in transcription elongation, e.g. pauses, are found to cause tangible effects in the degree of fluctuations in protein levels.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2105-12-121","type":"journal-article","created":{"date-parts":[[2011,4,27]],"date-time":"2011-04-27T18:18:27Z","timestamp":1303928307000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Stochastic sequence-level model of coupled transcription and translation in prokaryotes"],"prefix":"10.1186","volume":"12","author":[{"given":"Jarno","family":"M\u00e4kel\u00e4","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason","family":"Lloyd-Price","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olli","family":"Yli-Harja","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andre S","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2011,4,26]]},"reference":[{"issue":"3","key":"4524_CR1","doi-asserted-by":"publisher","first-page":"e1000704","DOI":"10.1371\/journal.pcbi.1000704","volume":"6","author":"T Rajala","year":"2010","unstructured":"Rajala T, H\u00e4kkinen A, Healy S, Yli-Harja O, Ribeiro AS: Effects of transcriptional pausing on gene expression dynamics. 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