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However comparative assessment of these features and furthermore effective and accurate methods are still in pressing need.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In this study, we first comprehensively collect the features to discriminate hot spots and non-hot spots and analyze their distributions. We find that hot spots have lower relASA and larger relative change in ASA, suggesting hot spots tend to be protected from bulk solvent. In addition, hot spots have more contacts including hydrogen bonds, salt bridges, and atomic contacts, which favor complexes formation. Interestingly, we find that conservation score and sequence entropy are not significantly different between hot spots and non-hot spots in Ab+ dataset (all complexes). While in Ab- dataset (antigen-antibody complexes are excluded), there are significant differences in two features between hot pots and non-hot spots. Secondly, we explore the predictive ability for each feature and the combinations of features by support vector machines (SVMs). The results indicate that sequence-based feature outperforms other combinations of features with reasonable accuracy, with a precision of 0.69, a recall of 0.68, an F1 score of 0.68, and an AUC of 0.68 on independent test set. Compared with other machine learning methods and two energy-based approaches, our approach achieves the best performance. Moreover, we demonstrate the applicability of our method to predict hot spots of two protein complexes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Experimental results show that support vector machine classifiers are quite effective in predicting hot spots based on sequence features. Hot spots cannot be fully predicted through simple analysis based on physicochemical characteristics, but there is reason to believe that integration of features and machine learning methods can remarkably improve the predictive performance for hot spots.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1471-2105-12-311","type":"journal-article","created":{"date-parts":[[2011,7,29]],"date-time":"2011-07-29T18:14:53Z","timestamp":1311963293000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Rigorous assessment and integration of the sequence and structure based features to predict hot spots"],"prefix":"10.1186","volume":"12","author":[{"given":"Ruoying","family":"Chen","sequence":"first","affiliation":[]},{"given":"Wenjing","family":"Chen","sequence":"additional","affiliation":[]},{"given":"Sixiao","family":"Yang","sequence":"additional","affiliation":[]},{"given":"Di","family":"Wu","sequence":"additional","affiliation":[]},{"given":"Yong","family":"Wang","sequence":"additional","affiliation":[]},{"given":"Yingjie","family":"Tian","sequence":"additional","affiliation":[]},{"given":"Yong","family":"Shi","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2011,7,29]]},"reference":[{"issue":"26","key":"4759_CR1","doi-asserted-by":"publisher","first-page":"26817","DOI":"10.1074\/jbc.M404020200","volume":"279","author":"S Elsasser","year":"2004","unstructured":"Elsasser S, Chandler-Militello D, Muller B, Hanna J, Finley D: Rad23 and Rpn10 serve as alternative ubiquitin receptors for the proteasome. 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