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So far methods have been developed to predict the common ligand binding residue positions for class A GPCRs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Here we present 1) ss-TEA, a method to identify specific ligand binding residue positions for any receptor, predicated on high quality sequence information. 2) The largest MSA of class A non olfactory GPCRs in the public domain consisting of 13324 sequences covering most of the species homologues of the human set of GPCRs. A set of ligand binding residue positions extracted from literature of 10 different receptors shows that our method has the best ligand binding residue prediction for 9 of these 10 receptors compared to another state-of-the-art method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The combination of the large multi species alignment and the newly introduced residue selection method ss-TEA can be used to rapidly identify subfamily specific ligand binding residues. This approach can aid the design of site directed mutagenesis experiments, explain receptor function and improve modelling. The method is also available online via GPCRDB at<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.gpcr.org\/7tm\/\" ext-link-type=\"uri\">http:\/\/www.gpcr.org\/7tm\/<\/jats:ext-link>.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1471-2105-12-332","type":"journal-article","created":{"date-parts":[[2011,8,10]],"date-time":"2011-08-10T18:16:00Z","timestamp":1313000160000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["ss-TEA: Entropy based identification of receptor specific ligand binding residues from a multiple sequence alignment of class A GPCRs"],"prefix":"10.1186","volume":"12","author":[{"given":"Marijn PA","family":"Sanders","sequence":"first","affiliation":[]},{"given":"Wilco WM","family":"Fleuren","sequence":"additional","affiliation":[]},{"given":"Stefan","family":"Verhoeven","sequence":"additional","affiliation":[]},{"given":"Sven","family":"van den Beld","sequence":"additional","affiliation":[]},{"given":"Wynand","family":"Alkema","sequence":"additional","affiliation":[]},{"given":"Jacob","family":"de Vlieg","sequence":"additional","affiliation":[]},{"given":"Jan PG","family":"Klomp","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2011,8,10]]},"reference":[{"issue":"10","key":"4729_CR1","doi-asserted-by":"publisher","first-page":"928","DOI":"10.1002\/1439-7633(20021004)3:10<928::AID-CBIC928>3.0.CO;2-5","volume":"3","author":"T Klabunde","year":"2002","unstructured":"Klabunde T, Hessler G: Drug design strategies for targeting G-protein-coupled receptors. 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