{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T21:21:41Z","timestamp":1776201701878,"version":"3.50.1"},"reference-count":17,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2012,6,18]],"date-time":"2012-06-18T00:00:00Z","timestamp":1339977600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BMC Bioinformatics"],"published-print":{"date-parts":[[2012,12]]},"DOI":"10.1186\/1471-2105-13-134","type":"journal-article","created":{"date-parts":[[2012,6,25]],"date-time":"2012-06-25T14:15:01Z","timestamp":1340633701000},"source":"Crossref","is-referenced-by-count":5049,"title":["Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction"],"prefix":"10.1186","volume":"13","author":[{"given":"Jian","family":"Ye","sequence":"first","affiliation":[]},{"given":"George","family":"Coulouris","sequence":"additional","affiliation":[]},{"given":"Irena","family":"Zaretskaya","sequence":"additional","affiliation":[]},{"given":"Ioana","family":"Cutcutache","sequence":"additional","affiliation":[]},{"given":"Steve","family":"Rozen","sequence":"additional","affiliation":[]},{"given":"Thomas L","family":"Madden","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2012,6,18]]},"reference":[{"issue":"5","key":"5274_CR1","doi-asserted-by":"publisher","first-page":"619","DOI":"10.2144\/000112776","volume":"44","author":"HD VanGuilder","year":"2008","unstructured":"VanGuilder HD, Vrana KE, Freeman WM: Twenty-five years of quantitative PCR for gene expression analysis. Biotechniques. 2008, 44 (5): 619-626.","journal-title":"Biotechniques"},{"issue":"2","key":"5274_CR2","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1016\/j.jcv.2005.02.010","volume":"34","author":"DM Whiley","year":"2005","unstructured":"Whiley DM, Sloots TP: Sequence variation in primer targets affects the accuracy of viral quantitative PCR. J Clin Virol. 2005, 34 (2): 104-107. 10.1016\/j.jcv.2005.02.010.","journal-title":"J Clin Virol"},{"issue":"2","key":"5274_CR3","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1111\/j.1574-6941.2007.00283.x","volume":"60","author":"R Sipos","year":"2007","unstructured":"Sipos R, Szekely AJ, Palatinszky M, Revesz S, Marialigeti K, Nikolausz M: Effect of primer mismatch, annealing temperature and PCR cycle number on 16\u2009S rRNA gene-targetting bacterial community analysis. FEMS Microbiol Ecol. 2007, 60 (2): 341-350. 10.1111\/j.1574-6941.2007.00283.x.","journal-title":"FEMS Microbiol Ecol"},{"issue":"5","key":"5274_CR4","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1016\/S0006-291X(02)02750-X","volume":"299","author":"CM Waterfall","year":"2002","unstructured":"Waterfall CM, Eisenthal R, Cobb BD: Kinetic characterisation of primer mismatches in allele-specific PCR: a quantitative assessment. Biochem Biophys Res Commun. 2002, 299 (5): 715-722. 10.1016\/S0006-291X(02)02750-X.","journal-title":"Biochem Biophys Res Commun"},{"issue":"20","key":"5274_CR5","doi-asserted-by":"publisher","first-page":"9370","DOI":"10.1021\/jf901976a","volume":"57","author":"R Ghedira","year":"2009","unstructured":"Ghedira R, Papazova N, Vuylsteke M, Ruttink T, Taverniers I, De Loose M: Assessment of primer\/template mismatch effects on real-time PCR amplification of target taxa for GMO quantification. J Agric Food Chem. 2009, 57 (20): 9370-9377. 10.1021\/jf901976a.","journal-title":"J Agric Food Chem"},{"issue":"17","key":"5274_CR6","doi-asserted-by":"publisher","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","volume":"25","author":"SF Altschul","year":"1997","unstructured":"Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25 (17): 3389-3402. 10.1093\/nar\/25.17.3389.","journal-title":"Nucleic Acids Res"},{"key":"5274_CR7","first-page":"365","volume":"132","author":"S Rozen","year":"2000","unstructured":"Rozen S, Skaletsky H: Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol. 2000, 132: 365-386.","journal-title":"Methods Mol Biol"},{"key":"5274_CR8","unstructured":"In-Silico PCR: , ,\n                    http:\/\/genome.ucsc.edu\/cgi-bin\/hgPcr?command=start\n                    \n                  ,"},{"issue":"Web Server issu","key":"5274_CR9","doi-asserted-by":"publisher","first-page":"W108","DOI":"10.1093\/nar\/gkh450","volume":"32","author":"K Rotmistrovsky","year":"2004","unstructured":"Rotmistrovsky K, Jang W, Schuler GD: A web server for performing electronic PCR. Nucleic Acids Res. 2004, 32 (Web Server issue): W108-W112.","journal-title":"Nucleic Acids Res"},{"key":"5274_CR10","doi-asserted-by":"publisher","first-page":"P11","DOI":"10.1186\/gb-2004-5-5-p11","volume":"5","author":"G Wrobel","year":"2004","unstructured":"Wrobel G, Kokocinski F, Lichter P: AutoPrime: selecting primers for expressed sequences. Genome Biol. 2004, 5: P11-10.1186\/gb-2004-5-5-p11.","journal-title":"Genome Biol"},{"key":"5274_CR11","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1186\/1471-2105-9-465","volume":"9","author":"S Arvidsson","year":"2008","unstructured":"Arvidsson S, Kwasniewski M, Riano-Pachon DM, Mueller-Roeber B: QuantPrime\u2013a flexible tool for reliable high-throughput primer design for quantitative PCR. BMC Bioinforma. 2008, 9: 465-10.1186\/1471-2105-9-465.","journal-title":"BMC Bioinforma"},{"key":"5274_CR12","unstructured":"PRIMEGENS: , ,\n                    http:\/\/primegens.org\/w3\/w32\/w3_html\/SSPD.html\n                    \n                  ,"},{"issue":"3","key":"5274_CR13","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1016\/0022-2836(70)90057-4","volume":"48","author":"SB Needleman","year":"1970","unstructured":"Needleman SB, Wunsch CD: A general method applicable to the search for similarities in the amino acid sequence of two proteins. J Mol Biol. 1970, 48 (3): 443-453. 10.1016\/0022-2836(70)90057-4.","journal-title":"J Mol Biol"},{"issue":"1\u20132","key":"5274_CR14","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1089\/10665270050081478","volume":"7","author":"Z Zhang","year":"2000","unstructured":"Zhang Z, Schwartz S, Wagner L, Miller W: A greedy algorithm for aligning DNA sequences. J Comput Biol. 2000, 7 (1\u20132): 203-214.","journal-title":"J Comput Biol"},{"issue":"Database issue","key":"5274_CR15","doi-asserted-by":"publisher","first-page":"D32","DOI":"10.1093\/nar\/gkn721","volume":"37","author":"KD Pruitt","year":"2009","unstructured":"Pruitt KD, Tatusova T, Klimke W, Maglott DR: NCBI Reference Sequences: current status, policy and new initiatives. Nucleic Acids Res. 2009, 37 (Database issue): D32-D36.","journal-title":"Nucleic Acids Res"},{"issue":"Database issue","key":"5274_CR16","doi-asserted-by":"publisher","first-page":"D792","DOI":"10.1093\/nar\/gkp1005","volume":"38","author":"A Spandidos","year":"2010","unstructured":"Spandidos A, Wang X, Wang H, Seed B: PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification. Nucleic Acids Res. 2010, 38 (Database issue): D792-D799.","journal-title":"Nucleic Acids Res"},{"key":"5274_CR17","unstructured":"PUBLIC DOMAIN NOTICE for NCBI:\n                    http:\/\/www.ncbi.nlm.nih.gov\/bookshelf\/br.fcgi?book=toolkit&part=toolkit.fm#A3\n                    \n                  ,"}],"container-title":["BMC Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2105-13-134.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/1471-2105-13-134\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2105-13-134.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,1,23]],"date-time":"2019-01-23T10:51:31Z","timestamp":1548240691000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcbioinformatics.biomedcentral.com\/articles\/10.1186\/1471-2105-13-134"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6,18]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["5274"],"URL":"https:\/\/doi.org\/10.1186\/1471-2105-13-134","relation":{},"ISSN":["1471-2105"],"issn-type":[{"value":"1471-2105","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6,18]]},"article-number":"134"}}