{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T16:13:21Z","timestamp":1770221601207,"version":"3.49.0"},"reference-count":57,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Bioinformatics"],"published-print":{"date-parts":[[2014,12]]},"DOI":"10.1186\/1471-2105-15-303","type":"journal-article","created":{"date-parts":[[2014,9,16]],"date-time":"2014-09-16T11:01:41Z","timestamp":1410865301000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["ECMIS: computational approach for the identification of hotspots at protein-protein interfaces"],"prefix":"10.1186","volume":"15","author":[{"given":"Prashant","family":"Shingate","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malini","family":"Manoharan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anshul","family":"Sukhwal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramanathan","family":"Sowdhamini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2014,9,16]]},"reference":[{"issue":"267","key":"6585_CR1","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1126\/science.7529940","volume":"1995","author":"T Clackson","year":"1995","unstructured":"Clackson T, Wells JA: A hot spot of binding energy in a hormone-receptor interface. Science. 1995, 1995 (267): 383-386.","journal-title":"Science"},{"issue":"280","key":"6585_CR2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1006\/jmbi.1998.1843","volume":"1998","author":"AA Bogan","year":"1998","unstructured":"Bogan AA, Thorn KS: Anatomy of hot spots in protein interfaces. J Mol Biol. 1998, 1998 (280): 1-9.","journal-title":"J Mol Biol"},{"key":"6585_CR3","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1186\/1471-2105-10-365","volume":"10","author":"S Lise","year":"2009","unstructured":"Lise S, Archambeau C, Pontil M, Jones DT: Prediction of hot spot residues at protein\u2013protein interfaces by combining machine learning and energy-based methods. BMC Bioinformatics. 2009, 10: 365-10.1186\/1471-2105-10-365.","journal-title":"BMC Bioinformatics"},{"key":"6585_CR4","doi-asserted-by":"publisher","first-page":"1281","DOI":"10.1016\/j.jmb.2004.10.077","volume":"345","author":"O Keskin","year":"2005","unstructured":"Keskin O, Ma B, Nussinov R: Hot regions in protein\u2013protein interactions: the organization and contribution of structurally conserved hot spot residues. J Mol Biol. 2005, 345: 1281-1294. 10.1016\/j.jmb.2004.10.077.","journal-title":"J Mol Biol"},{"key":"6585_CR5","doi-asserted-by":"publisher","first-page":"803","DOI":"10.1002\/prot.21396","volume":"68","author":"IS Moreira","year":"2007","unstructured":"Moreira IS, Fernandes PA, Ramos MJ: Hot spots\u2014a review of the protein\u2013protein interface determinant amino-acid residues. Proteins. 2007, 68: 803-812. 10.1002\/prot.21396.","journal-title":"Proteins"},{"key":"6585_CR6","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1093\/bioinformatics\/17.3.284","volume":"17","author":"KS Thorn","year":"2001","unstructured":"Thorn KS, Bogan AA: ASEdb: a database of alanine mutations and their effects on the free energy of binding in protein interactions. Bioinformatics. 2001, 17: 284-285. 10.1093\/bioinformatics\/17.3.284.","journal-title":"Bioinformatics"},{"key":"6585_CR7","doi-asserted-by":"publisher","first-page":"1453","DOI":"10.1093\/bioinformatics\/btg163","volume":"19","author":"TB Fischer","year":"2003","unstructured":"Fischer TB, Arunachalam KV, Bailey D, Mangual V, Bakhru S, Russo R, Huang D, Paczkowski M, Lalchandani V, Ramachandra C, Ellison B, Galer S, Shapley J, Fuentes E, Tsai J: The binding interface database (BID): a compilation of amino acid hot spots in protein interfaces. Bioinformatics. 2003, 19: 1453-1454. 10.1093\/bioinformatics\/btg163.","journal-title":"Bioinformatics"},{"issue":"11","key":"6585_CR8","doi-asserted-by":"publisher","first-page":"1335","DOI":"10.1093\/bioinformatics\/btl079","volume":"22","author":"N Burgoyne","year":"2006","unstructured":"Burgoyne N, Jackson R: Predicting protein interaction sites: binding hotspots in protein-protein and protein-ligand interfaces. Bioinformatics. 2006, 22 (11): 1335-1342. 10.1093\/bioinformatics\/btl079.","journal-title":"Bioinformatics"},{"issue":"43","key":"6585_CR9","doi-asserted-by":"publisher","first-page":"15447","DOI":"10.1073\/pnas.0505425102","volume":"102","author":"M Guharoy","year":"2005","unstructured":"Guharoy M, Chakrabarti P: Conservation and relative importance of residues across protein-protein interfaces. Proc Natl Acad Sci. 2005, 102 (43): 15447-15452. 10.1073\/pnas.0505425102.","journal-title":"Proc Natl Acad Sci"},{"issue":"6","key":"6585_CR10","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1093\/bioinformatics\/btp058","volume":"25","author":"J Li","year":"2009","unstructured":"Li J, Liu Q: \u2018Double water exclusion\u2019: a hypothesis refining the O-ring theory for the hot spots at protein interfaces. Bioinformatics. 2009, 25 (6): 743-750. 10.1093\/bioinformatics\/btp058.","journal-title":"Bioinformatics"},{"issue":"3","key":"6585_CR11","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1002\/prot.22583","volume":"78","author":"Q Liu","year":"2010","unstructured":"Liu Q, Li J: Propensity vectors of low-ASA residue pairs in the distinction of protein interactions. Proteins. 2010, 78 (3): 589-602.","journal-title":"Proteins"},{"key":"6585_CR12","doi-asserted-by":"publisher","first-page":"2455","DOI":"10.1002\/pro.5560031231","volume":"3","author":"NV Grishin","year":"1994","unstructured":"Grishin NV, Phillips MA: The subunit interfaces of oligomeric enzymes are conserved to a similar extent to the overall protein sequences. Protein Sci. 1994, 3: 2455-2458. 10.1002\/pro.5560031231.","journal-title":"Protein Sci"},{"key":"6585_CR13","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1002\/1097-0134(20010101)42:1<108::AID-PROT110>3.0.CO;2-O","volume":"42","author":"WS Valdar","year":"2001","unstructured":"Valdar WS, Thornton JM: Protein-protein interfaces: analysis of amino acid conservation in homodimers. Proteins. 2001, 42: 108-124. 10.1002\/1097-0134(20010101)42:1<108::AID-PROT110>3.0.CO;2-O.","journal-title":"Proteins"},{"key":"6585_CR14","doi-asserted-by":"publisher","first-page":"750","DOI":"10.1126\/science.1068696","volume":"296","author":"HB Fraser","year":"2002","unstructured":"Fraser HB, Hirsh AE, Steinmetz LM, Scharfe C, Feldman MW: Evolutionary rate in the protein interaction network. Science. 2002, 296: 750-752. 10.1126\/science.1068696.","journal-title":"Science"},{"key":"6585_CR15","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1110\/ps.03323604","volume":"13","author":"DR Caffrey","year":"2004","unstructured":"Caffrey DR, Somaroo S, Hughes JD, Mintseris J, Huang HS: Are protein-protein interfaces more conserved in sequence than the rest of the protein surface?. Protein Sci. 2004, 13: 190-202. 10.1110\/ps.03323604.","journal-title":"Protein Sci"},{"key":"6585_CR16","doi-asserted-by":"publisher","first-page":"884","DOI":"10.1110\/ps.03465504","volume":"13","author":"AR Panchenko","year":"2004","unstructured":"Panchenko AR, Kondrashov F, Bryant S: Prediction of functional sites by analysis of sequence and structure conservation. Protein Sci. 2004, 13: 884-892. 10.1110\/ps.03465504.","journal-title":"Protein Sci"},{"key":"6585_CR17","doi-asserted-by":"publisher","first-page":"3475","DOI":"10.1529\/biophysj.107.114835","volume":"94","author":"ON Yogurtcu","year":"2008","unstructured":"Yogurtcu ON, Erdemli SB, Nussinov R, Turkay M, Keskin O: Restricted mobility of conserved residues in protein-protein interfaces in molecular simulations. Biophys J. 2008, 94: 3475-3485. 10.1529\/biophysj.107.114835.","journal-title":"Biophys J"},{"key":"6585_CR18","doi-asserted-by":"publisher","first-page":"1283","DOI":"10.1016\/j.jmb.2005.03.051","volume":"348","author":"J Kim","year":"2005","unstructured":"Kim J, Mao J, Gunner MR: Are acidic and basic groups in buried proteins predicted to be ionized?. J Mol Biol. 2005, 348: 1283-1298. 10.1016\/j.jmb.2005.03.051.","journal-title":"J Mol Biol"},{"key":"6585_CR19","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1093\/bioinformatics\/19.1.163","volume":"19","author":"F Glaser","year":"2003","unstructured":"Glaser F, Pupko T, Paz I, Bell RE, Bechor-Shental D, Martz E, Ben-Tal N: ConSurf: identification of functional regions in proteins by surface-mapping of phylogenetic information. Bioinformatics. 2003, 19: 163-164. 10.1093\/bioinformatics\/19.1.163.","journal-title":"Bioinformatics"},{"key":"6585_CR20","doi-asserted-by":"publisher","first-page":"390","DOI":"10.1016\/0076-6879(91)02020-A","volume":"202","author":"JA Wells","year":"1991","unstructured":"Wells JA: Systematic mutational analyses of protein-protein interfaces. Methods Enzymol. 1991, 202: 390-411.","journal-title":"Methods Enzymol"},{"key":"6585_CR21","doi-asserted-by":"publisher","first-page":"2351","DOI":"10.1002\/pro.5560031219","volume":"3","author":"L Jin","year":"1994","unstructured":"Jin L, Wells JA: Dissecting the energetics of an antibody-antigen interface by alanine shaving and molecular grafting. Protein Sci. 1994, 3: 2351-2357. 10.1002\/pro.5560031219.","journal-title":"Protein Sci"},{"key":"6585_CR22","doi-asserted-by":"publisher","first-page":"14116","DOI":"10.1073\/pnas.202485799","volume":"99","author":"T Kortemme","year":"2002","unstructured":"Kortemme T, Baker D: A simple physical model for binding energy hot spots in protein-protein complexes. Proc Natl Acad Sci U S A. 2002, 99: 14116-14121. 10.1073\/pnas.202485799.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"6585_CR23","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1016\/S0022-2836(02)00442-4","volume":"320","author":"R Guerois","year":"2002","unstructured":"Guerois R, Nielsen JE, Serrano L: Predicting changes in the stability of proteins and protein complexes: a study of more than 1000 mutations. J Mol Biol. 2002, 320: 369-387. 10.1016\/S0022-2836(02)00442-4.","journal-title":"J Mol Biol"},{"issue":"Web Server issu","key":"6585_CR24","doi-asserted-by":"publisher","first-page":"W265","DOI":"10.1093\/nar\/gkn346","volume":"36","author":"SJ Darnell","year":"2008","unstructured":"Darnell SJ, LeGault L, Mitchell JC: KFC Server: interactive forecasting of protein interaction hot spots. Nucleic Acids Res. 2008, 36 (Web Server issue): W265-W269.","journal-title":"Nucleic Acids Res"},{"key":"6585_CR25","doi-asserted-by":"publisher","first-page":"2672","DOI":"10.1093\/nar\/gkp132","volume":"37","author":"KI Cho","year":"2009","unstructured":"Cho KI, Kim D, Lee D: A feature-based approach to modelling protein\u2013protein interaction hot spots. Nucleic Acids Res. 2009, 37: 2672-2687. 10.1093\/nar\/gkp132.","journal-title":"Nucleic Acids Res"},{"issue":"Suppl","key":"6585_CR26","doi-asserted-by":"publisher","first-page":"W402","DOI":"10.1093\/nar\/gkq323","volume":"38","author":"N Tuncbag","year":"2010","unstructured":"Tuncbag N, Keskin O, Gursoy A: HotPoint: hot spot prediction server for protein interfaces. Nucleic Acids Res. 2010, 38 (Suppl): W402-W406.","journal-title":"Nucleic Acids Res"},{"key":"6585_CR27","doi-asserted-by":"publisher","first-page":"2671","DOI":"10.1002\/prot.23094","volume":"79","author":"X Zhu","year":"2011","unstructured":"Zhu X, Mitchell JC: KFC2: a knowledge-based hot spot prediction method based on interface solvation, atomic density, and plasticity features. Proteins. 2011, 79: 2671-2683. 10.1002\/prot.23094.","journal-title":"Proteins"},{"key":"6585_CR28","doi-asserted-by":"publisher","first-page":"1231","DOI":"10.1021\/jm061134b","volume":"50","author":"MR Landon","year":"2007","unstructured":"Landon MR, Lancia DR, Yu J, Thiel SC, Vajda S: Identification of hot spots within druggable binding regions by computational solvent mapping of proteins. J Med Chem. 2007, 50: 1231-1240. 10.1021\/jm061134b.","journal-title":"J Med Chem"},{"key":"6585_CR29","doi-asserted-by":"publisher","first-page":"D662","DOI":"10.1093\/nar\/gkm813","volume":"36","author":"E Guney","year":"2008","unstructured":"Guney E, Tuncbag N, Keskin O, Gursoy A: HotSprint: database of computational hot spots in protein interfaces. Nucleic Acids Res. 2008, 36: D662-D666.","journal-title":"Nucleic Acids Res"},{"key":"6585_CR30","doi-asserted-by":"publisher","first-page":"1513","DOI":"10.1093\/bioinformatics\/btp240","volume":"25","author":"N Tuncbag","year":"2009","unstructured":"Tuncbag N, Gursoy A, Keskin O: Identification of computational hot spots in protein interfaces: combining solvent accessibility and inter-residue potentials improves the accuracy. Bioinformatics. 2009, 25: 1513-1520. 10.1093\/bioinformatics\/btp240.","journal-title":"Bioinformatics"},{"key":"6585_CR31","doi-asserted-by":"publisher","first-page":"121","DOI":"10.6026\/97320630001121","volume":"1","author":"L Li","year":"2006","unstructured":"Li L, Zhao B, Cui Z, Gan J, Sakharkar MK, Kangueane P: Identification of hot spot residues at protein-protein interface. Bioinformation. 2006, 1: 121-126. 10.6026\/97320630001121. 18","journal-title":"Bioinformation"},{"key":"6585_CR32","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1016\/j.jmb.2004.09.051","volume":"344","author":"X Li","year":"2004","unstructured":"Li X, Keskin O, Ma B, Nussinov R, Liang J: Protein-protein interactions: hot spots and structurally conserved residues often locate in complemented pockets that pre-organized in the unbound states: implications for docking. J Mol Biol. 2004, 344: 781-795. 10.1016\/j.jmb.2004.09.051.","journal-title":"J Mol Biol"},{"key":"6585_CR33","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1002\/(SICI)1097-0134(20000601)39:4<331::AID-PROT60>3.0.CO;2-A","volume":"39","author":"Z Hu","year":"2000","unstructured":"Hu Z, Ma B, Wolfson H, Nussinov R: Conservation of polar residues as hot spots at protein interfaces. Proteins. 2000, 39: 331-342. 10.1002\/(SICI)1097-0134(20000601)39:4<331::AID-PROT60>3.0.CO;2-A.","journal-title":"Proteins"},{"key":"6585_CR34","doi-asserted-by":"publisher","first-page":"5772","DOI":"10.1073\/pnas.1030237100","volume":"100","author":"B Ma","year":"2003","unstructured":"Ma B, Elkayam T, Wolfson H, Nussinov R: Protein-protein interactions: structurally conserved residues distinguish between binding sites and exposed protein surfaces. Proc Natl Acad Sci U S A. 2003, 100: 5772-5777. 10.1073\/pnas.1030237100.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"6585_CR35","doi-asserted-by":"publisher","first-page":"999","DOI":"10.2174\/156802607780906717","volume":"7","author":"B Ma","year":"2007","unstructured":"Ma B, Nussinov R: Trp\/Met\/Phe hot spots in protein-protein interactions: potential targets in drug design. Curr Top Med Chem. 2007, 7: 999-1005. 10.2174\/156802607780906717.","journal-title":"Curr Top Med Chem"},{"key":"6585_CR36","doi-asserted-by":"publisher","first-page":"e119","DOI":"10.1371\/journal.pcbi.0030119","volume":"3","author":"Y Ofran","year":"2007","unstructured":"Ofran Y, Rost B: Protein-protein interaction hotspots carved into sequences. PLoS Comput Biol. 2007, 3: e119-10.1371\/journal.pcbi.0030119.","journal-title":"PLoS Comput Biol"},{"issue":"Suppl. 5","key":"6585_CR37","doi-asserted-by":"publisher","first-page":"S8","DOI":"10.1186\/1471-2105-8-S5-S8","volume":"8","author":"CM Hsu","year":"2007","unstructured":"Hsu CM, Chen CY, Liu BJ, Huang CC, Laio MH, Lin CC, Wu TL: Identification of hot regions in protein-protein interactions by sequential pattern mining. BMC Bioinformatics. 2007, 8 (Suppl. 5): S8-","journal-title":"BMC Bioinformatics"},{"key":"6585_CR38","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/S0959-440X(02)00283-X","volume":"12","author":"WL DeLano","year":"2002","unstructured":"DeLano WL: Unraveling hot spots in binding interfaces: progress and challenges. Curr Opin Struct Biol. 2002, 12: 14-20. 10.1016\/S0959-440X(02)00283-X.","journal-title":"Curr Opin Struct Biol"},{"key":"6585_CR39","doi-asserted-by":"publisher","first-page":"2329","DOI":"10.1002\/prot.22745","volume":"78","author":"DJ Diller","year":"2010","unstructured":"Diller DJ, Humblet C, Zhang X, Westerhoff LM: Computational alanine scanning with linear scaling semiempirical quantum mechanical methods. Proteins. 2010, 78: 2329-2337. 10.1002\/prot.22745.","journal-title":"Proteins"},{"key":"6585_CR40","doi-asserted-by":"publisher","first-page":"8133","DOI":"10.1021\/ja990935j","volume":"121","author":"I Massova","year":"1999","unstructured":"Massova I, Kollman PA: Computational alanine scanning to probe protein-protein interactions: a novel approach to evaluate binding free energies. J Am Chem Soc. 1999, 121: 8133-8139. 10.1021\/ja990935j.","journal-title":"J Am Chem Soc"},{"key":"6585_CR41","doi-asserted-by":"publisher","first-page":"e86","DOI":"10.1093\/nar\/gkp1158","volume":"38","author":"SA Assi","year":"2010","unstructured":"Assi SA, Tanaka T, Rabbitts TH, Fernandez-Fuentes N: PCRPi: presaging critical residues in protein interfaces, a new computational tool to chart hot spots in protein interfaces. Nucleic Acids Res. 2010, 38: e86-10.1093\/nar\/gkp1158.","journal-title":"Nucleic Acids Res"},{"key":"6585_CR42","doi-asserted-by":"publisher","first-page":"813","DOI":"10.1002\/prot.21474","volume":"68","author":"SJ Darnell","year":"2007","unstructured":"Darnell SJ, Page D, Mitchell JC: An automated decision-tree approach to predicting protein interaction hot spots. Proteins. 2007, 68: 813-823. 10.1002\/prot.21474.","journal-title":"Proteins"},{"issue":"3","key":"6585_CR43","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1093\/protein\/gzr066","volume":"25","author":"L Wang","year":"2012","unstructured":"Wang L, Liu ZP, Zhang XS, Chen L: Prediction of hot spots in protein interfaces using a random forest model with hybrid features. Protein Eng Des Sel. 2012, 25 (3): 119-126. 10.1093\/protein\/gzr066.","journal-title":"Protein Eng Des Sel"},{"key":"6585_CR44","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1186\/1471-2105-9-447","volume":"9","author":"S Grosdidier","year":"2008","unstructured":"Grosdidier S, Fernandez-Recio J: Identification of hot spot residues in protein\u2013protein interactions by computational docking. BMC Bioinformatics. 2008, 9: 447-10.1186\/1471-2105-9-447.","journal-title":"BMC Bioinformatics"},{"key":"6585_CR45","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1007\/s00894-003-0168-3","volume":"10","author":"Y Gao","year":"2004","unstructured":"Gao Y, Wang R, Lai L: Structure-based method for analyzing protein-protein interfaces. J Mol Model. 2004, 10: 44-54. 10.1007\/s00894-003-0168-3.","journal-title":"J Mol Model"},{"key":"6585_CR46","doi-asserted-by":"publisher","first-page":"2607","DOI":"10.2174\/092986706778201530","volume":"13","author":"D Gonzalez-Ruiz","year":"2006","unstructured":"Gonzalez-Ruiz D, Gohlke H: Targeting protein-protein interactions with small molecules: challenges and perspectives for computational binding epitope detection and ligand finding. Curr Med Chem. 2006, 13: 2607-2625. 10.2174\/092986706778201530.","journal-title":"Curr Med Chem"},{"key":"6585_CR47","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1186\/1471-2105-11-174","volume":"11","author":"JF Xia","year":"2010","unstructured":"Xia JF, Zhao XM, Song J, Huang DS: APIS: accurate prediction of hot spots in protein interfaces by combining protrusion index with solvent accessibility. BMC Bioinformatics. 2010, 11: 174-10.1186\/1471-2105-11-174.","journal-title":"BMC Bioinformatics"},{"key":"6585_CR48","doi-asserted-by":"publisher","first-page":"1652","DOI":"10.1039\/c3mb25484d","volume":"9","author":"A Sukhwal","year":"2013","unstructured":"Sukhwal A, Sowdhamini R: Oligomerisation status and evolutionary conservation of interface of protein structural domain superfamilies. Mol BioSyst. 2013, 9: 1652-1661. 10.1039\/c3mb25484d.","journal-title":"Mol BioSyst"},{"key":"6585_CR49","doi-asserted-by":"publisher","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","volume":"25","author":"SF Altschul","year":"1997","unstructured":"Altschul SF, Madden TL, Sch\u00e4ffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25: 3389-3402. 10.1093\/nar\/25.17.3389.","journal-title":"Nucleic Acids Res"},{"key":"6585_CR50","doi-asserted-by":"publisher","first-page":"1658","DOI":"10.1093\/bioinformatics\/btl158","volume":"22","author":"W Li","year":"2006","unstructured":"Li W, Godzik A: Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences. Bioinformatics. 2006, 22: 1658-1659. 10.1093\/bioinformatics\/btl158.","journal-title":"Bioinformatics"},{"issue":"21","key":"6585_CR51","doi-asserted-by":"publisher","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","volume":"23","author":"MA Larkin","year":"2007","unstructured":"Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG: ClustalW and ClustalX version 2. Bioinformatics. 2007, 23 (21): 2947-2948. 10.1093\/bioinformatics\/btm404.","journal-title":"Bioinformatics"},{"key":"6585_CR52","doi-asserted-by":"publisher","first-page":"716","DOI":"10.1006\/jmbi.1993.1548","volume":"233","author":"MS Johnson","year":"1993","unstructured":"Johnson MS, Overington JP, Blundell TL: A structural basis for sequence comparisons: an evaluation of scoring methodologies. J Mol Biol. 1993, 233: 716-738. 10.1006\/jmbi.1993.1548.","journal-title":"J Mol Biol"},{"issue":"5","key":"6585_CR53","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1093\/bioinformatics\/btl679","volume":"23","author":"G Pugalenthi","year":"2007","unstructured":"Pugalenthi G, Suganthan PN, Sowdhamini R, Chakrabarti S: SMotif: a server for structural motifs in proteins. Bioinformatics. 2007, 23 (5): 637-638. 10.1093\/bioinformatics\/btl679.","journal-title":"Bioinformatics"},{"key":"6585_CR54","doi-asserted-by":"publisher","first-page":"476","DOI":"10.1021\/bi971884a","volume":"37","author":"R Wallis","year":"1998","unstructured":"Wallis R, Leung KY, Osborne MJ, James R, Moore GR, Kleanthous C: Specificity in protein\u2013protein recognition: conserved Im9 residues are the major determinants of stability in the colicin E9 dnase Im9 complex. Biochemistry. 1998, 37: 476-485. 10.1021\/bi971884a.","journal-title":"Biochemistry"},{"key":"6585_CR55","doi-asserted-by":"publisher","first-page":"2727","DOI":"10.1111\/j.1365-2958.1991.tb01981.x","volume":"5","author":"MD Curtis","year":"1991","unstructured":"Curtis MD, James R: Investigation of the specificity of the interaction between colicin E9 and its immunity protein by site-directed mutagenesis. Mol Microbiol. 1991, 5: 2727-2733. 10.1111\/j.1365-2958.1991.tb01981.x.","journal-title":"Mol Microbiol"},{"issue":"18","key":"6585_CR56","doi-asserted-by":"publisher","first-page":"6823","DOI":"10.1021\/bi047301w","volume":"44","author":"ES Radisky","year":"2005","unstructured":"Radisky ES, Lu CJK, Kwan G, Koshland DE: Role of the intermolecular hydrogen bond network in the inhibitory power of chymotrypsin inhibitor 2. Biochemistry. 2005, 44 (18): 6823-6830. 10.1021\/bi047301w.","journal-title":"Biochemistry"},{"issue":"43","key":"6585_CR57","doi-asserted-by":"publisher","first-page":"13648","DOI":"10.1021\/bi048797k","volume":"43","author":"ES Radisky","year":"2004","unstructured":"Radisky ES, Kwan G, Lu CJK, Koshland DE: Binding, proteolytic, and crystallographic analyses of mutations at the protease\u2009\u2212\u2009inhibitor interface of the subtilisin BPN\u2018\/chymotrypsin inhibitor 2 complex. Biochemistry. 2004, 43 (43): 13648-13656. 10.1021\/bi048797k.","journal-title":"Biochemistry"}],"container-title":["BMC Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2105-15-303.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,2]],"date-time":"2021-09-02T05:50:33Z","timestamp":1630561833000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcbioinformatics.biomedcentral.com\/articles\/10.1186\/1471-2105-15-303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,16]]},"references-count":57,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["6585"],"URL":"https:\/\/doi.org\/10.1186\/1471-2105-15-303","relation":{},"ISSN":["1471-2105"],"issn-type":[{"value":"1471-2105","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,16]]},"assertion":[{"value":"27 January 2014","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 August 2014","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 September 2014","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"303"}}