{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T03:16:01Z","timestamp":1780110961532,"version":"3.54.0"},"reference-count":16,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2004,9,9]],"date-time":"2004-09-09T00:00:00Z","timestamp":1094688000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0"},{"start":{"date-parts":[[2004,9,9]],"date-time":"2004-09-09T00:00:00Z","timestamp":1094688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Bioinformatics"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The discovery of cis-regulatory modules in metazoan genomes is crucial for understanding the connection between genes and organism diversity. It is important to quantify how comparative genomics can improve computational detection of such modules.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We run the Stubb software on the entire D. melanogaster genome, to obtain predictions of modules involved in segmentation of the embryo. Stubb uses a probabilistic model to score sequences for clustering of transcription factor binding sites, and can exploit multiple species data within the same probabilistic framework. The predictions are evaluated using publicly available gene expression data for thousands of genes, after careful manual annotation. We demonstrate that the use of a second genome (D. pseudoobscura) for cross-species comparison significantly improves the prediction accuracy of Stubb, and is a more sensitive approach than intersecting the results of separate runs over the two genomes. The entire list of predictions is made available online.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Evolutionary conservation of modules serves as a filter to improve their detection\n                      <jats:italic>in silico<\/jats:italic>\n                      . The future availability of additional fruitfly genomes therefore carries the prospect of highly specific genome-wide predictions using Stubb.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/1471-2105-5-129","type":"journal-article","created":{"date-parts":[[2004,9,14]],"date-time":"2004-09-14T02:23:39Z","timestamp":1095128619000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":73,"title":["Cross-species comparison significantly improves genome-wide prediction of cis-regulatory modules in Drosophila"],"prefix":"10.1186","volume":"5","author":[{"given":"Saurabh","family":"Sinha","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark D","family":"Schroeder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Unnerstall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrike","family":"Gaul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric D","family":"Siggia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2004,9,9]]},"reference":[{"issue":"2","key":"245_CR1","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1073\/pnas.231608898","volume":"99","author":"BP Berman","year":"2002","unstructured":"Berman BP, Nibu Y, Pfeiffer BD, Tomancak P, Celniker SE, Levine M, Rubin GM, Eisen MB: Exploiting transcription factor binding site clustering to identify cis-regulatory modules involved in pattern formation in the Drosophila genome.\n                           Proc Natl Acad Sci U S A 2002, 99(2):757\u201362. 10.1073\/pnas.231608898","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"245_CR2","doi-asserted-by":"publisher","first-page":"763","DOI":"10.1073\/pnas.012591199","volume":"99","author":"M Markstein","year":"2002","unstructured":"Markstein M, Markstein P, Markstein V, Levine MS: Genome-wide analysis of clustered Dorsal binding sites identifies putative target genes in the Drosophila embryo.\n                           Proc Natl Acad Sci U S A 2002, 99(2):763\u20138. 10.1073\/pnas.012591199","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"7","key":"245_CR3","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1101\/gr.228902","volume":"12","author":"MS Halfon","year":"2002","unstructured":"Halfon MS, Grad Y, Church GM, Michelson AM: Computation-based discovery of related transcriptional regulatory modules and motifs using an experimentally validated combinatorial model.\n                      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