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To provide researchers with a sufficient number of pre-designed specific RT-PCR primer pairs for whole genes in Arabidopsis, we aimed to construct a genome-wide primer-pair database.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Description<\/jats:title>\n            <jats:p>We considered the homogeneous physical and chemical properties of each primer (homogeneity) of a gene, non-specific binding against all other known genes (specificity), and other possible amplicons from its corresponding genomic DNA or similar cDNAs (additional information). Then, we evaluated the reliability of our database with selected primer pairs from 15 genes using conventional and real time RT-PCR.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Approximately 97% of 28,952 genes investigated were finally registered in <jats:italic>AtRTPrimer<\/jats:italic>. Unlike other freely available primer databases for <jats:italic>Arabidopsis thaliana<\/jats:italic>, <jats:italic>AtRTPrimer<\/jats:italic> provides a large number of reliable primer pairs for each gene so that researchers can perform various types of RT-PCR experiments for their specific needs. Furthermore, by experimentally evaluating our database, we made sure that our database provides good starting primer pairs for Arabidopsis researchers to perform various types of RT-PCR experiments.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2105-7-179","type":"journal-article","created":{"date-parts":[[2006,4,6]],"date-time":"2006-04-06T12:52:02Z","timestamp":1144327922000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["AtRTPrimer: database for Arabidopsis genome-wide homogeneous and specific RT-PCR primer-pairs"],"prefix":"10.1186","volume":"7","author":[{"given":"Sangjo","family":"Han","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongsup","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2006,3,30]]},"reference":[{"issue":"17","key":"918_CR1","doi-asserted-by":"publisher","first-page":"14363","DOI":"10.1074\/jbc.C200101200","volume":"277","author":"MJ Holland","year":"2002","unstructured":"Holland MJ: Transcript abundance in yeast varies over six orders of magnitude. 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