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Therefore, amyloid aggregation modulating ligands are expected to serve as therapeutic medicines.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We screened peptide ligands against \u03b1-synuclein by <jats:italic>in silico<\/jats:italic> panning, a method which we have proposed previously. In this study, we selected as the target a very hydrophobic region known as the amyloid-core-forming region. Since this region cannot be dissolved in water, it is difficult to carry out the <jats:italic>in vitro<\/jats:italic> screening of its peptide ligand. We carried out 6 rounds of <jats:italic>in silico<\/jats:italic> panning using a genetic algorithm and a docking simulation. After the <jats:italic>in silico<\/jats:italic> panning, we evaluated the top peptides screened <jats:italic>in silico<\/jats:italic> by <jats:italic>in vitro<\/jats:italic> assay. These peptides were capable of binding to \u03b1-synuclein.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>We demonstrated that it is possible to screen \u03b1-synuclein-binding peptides by <jats:italic>in silico<\/jats:italic> panning. The screened peptides bind to \u03b1-synuclein, thus affecting the aggregation of \u03b1-synuclein.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2105-8-451","type":"journal-article","created":{"date-parts":[[2007,11,16]],"date-time":"2007-11-16T07:13:32Z","timestamp":1195197212000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Peptide ligand screening of \u03b1-synuclein aggregation modulators by in silico panning"],"prefix":"10.1186","volume":"8","author":[{"given":"Koichi","family":"Abe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natsuki","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Sode","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazunori","family":"Ikebukuro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2007,11,16]]},"reference":[{"issue":"11","key":"1823_CR1","doi-asserted-by":"publisher","first-page":"6469","DOI":"10.1073\/pnas.95.11.6469","volume":"95","author":"MG Spillantini","year":"1998","unstructured":"Spillantini MG, Crowther RA, Jakes R, Hasegawa M, Goedert M: alpha-Synuclein in filamentous inclusions of Lewy bodies from Parkinson's disease and dementia with lewy bodies. 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