{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T08:43:12Z","timestamp":1779352992968,"version":"3.51.4"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Cell Biol"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>DYT1 dystonia is an autosomal dominant neurological condition caused by a mutation that removes a single glutamic acid residue (\u0394E) from the torsinA (torA) AAA+ protein. TorA appears to possess a nuclear envelope (NE) localized activity that requires Lamina-Associated-Polypeptide 1 (LAP1), which is an inner nuclear membrane localized torA-binding partner. Although hypoactive, the DYT1 dystonia torA-\u0394E isoform often concentrates in the NE, suggesting that torA-\u0394E also interacts with an NE-localized binding partner.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We confirm that NE-localized torA-\u0394E does not co-immunoprecipitate with LAP1, and find that torA-\u0394E continues to concentrate in the NE of cells that lack LAP1. Instead, we find that variability in torA-\u0394E localization correlates with the presence of the SUN-domain and Nesprin proteins that assemble into the LINC complex. We also find that siRNA depletion of SUN1, but not other LINC complex components, removes torA-\u0394E from the NE. In contrast, the LAP1-dependent NE-accumulation of an ATP-locked torA mutant is unaffected by loss of LINC complex proteins. This SUN1 dependent torA-\u0394E localization requires the torA membrane association domain, as well as a putative substrate-interaction residue, Y147, neither of which are required for torA interaction with LAP1. We also find that mutation of these motifs, or depletion of SUN1, decreases the amount of torA-WT that colocalizes with NE markers, indicating that each also underlies a normal NE-localized torA binding interaction.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>These data suggest that the disease causing \u0394E mutation promotes an association between torA and SUN1 that is distinct to the interaction between LAP1 and ATP-bound torA. This evidence for two NE-localized binding partners suggests that torA may act on multiple substrates and\/or possesses regulatory co-factor partners. In addition, finding that the DYT1 mutation causes abnormal association with SUN1 implicates LINC complex dysfunction in DYT1 dystonia pathogenesis, and suggests a gain-of-function activity contributes to this dominantly inherited disease.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2121-12-24","type":"journal-article","created":{"date-parts":[[2011,6,1]],"date-time":"2011-06-01T06:20:15Z","timestamp":1306909215000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["The nuclear envelope localization of DYT1 dystonia torsinA-\u0394E requires the SUN1 LINC complex component"],"prefix":"10.1186","volume":"12","author":[{"given":"Michael T","family":"Jungwirth","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dhivya","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle Y","family":"Jeong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rose E","family":"Goodchild","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2011,5,31]]},"reference":[{"key":"568_CR1","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1038\/nrneurol.2009.160","volume":"5","author":"LM Tanabe","year":"2009","unstructured":"Tanabe LM, Kim CE, Alagem N, Dauer WT: Primary dystonia: molecules and mechanisms. Nat Rev Neurol. 2009, 5: 598-609. 10.1038\/nrneurol.2009.160.","journal-title":"Nat Rev Neurol"},{"key":"568_CR2","doi-asserted-by":"publisher","first-page":"2005","DOI":"10.1093\/brain\/awp172","volume":"132","author":"U Muller","year":"2009","unstructured":"Muller U: The monogenic primary dystonias. Brain. 2009, 132: 2005-2025. 10.1093\/brain\/awp172.","journal-title":"Brain"},{"key":"568_CR3","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1038\/ng0997-40","volume":"17","author":"LJ Ozelius","year":"1997","unstructured":"Ozelius LJ, Hewett JW, Page CE, Bressman SB, Kramer PL, Shalish C, de Leon D, Brin MF, Raymond D, Corey DP, Fahn S, Risch NJ, Buckler AJ, Gusella JF, Breakefield XO: The early-onset torsion dystonia gene (DYT1) encodes an ATP-binding protein. Nat Genet. 1997, 17: 40-48. 10.1038\/ng0997-40.","journal-title":"Nat Genet"},{"key":"568_CR4","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1016\/j.neuron.2005.11.010","volume":"48","author":"RE Goodchild","year":"2005","unstructured":"Goodchild RE, Kim CE, Dauer WT: Loss of the Dystonia-Associated Protein TorsinA Selectively Disrupts the Neuronal Nuclear Envelope. Neuron. 2005, 48: 923-932. 10.1016\/j.neuron.2005.11.010.","journal-title":"Neuron"},{"key":"568_CR5","doi-asserted-by":"publisher","first-page":"1657","DOI":"10.1111\/j.1600-0854.2007.00642.x","volume":"8","author":"SR White","year":"2007","unstructured":"White SR, Lauring B: AAA+ ATPases: achieving diversity of function with conserved machinery. Traffic. 2007, 8: 1657-1667. 10.1111\/j.1600-0854.2007.00642.x.","journal-title":"Traffic"},{"key":"568_CR6","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1038\/nrm1684","volume":"6","author":"PI Hanson","year":"2005","unstructured":"Hanson PI, Whiteheart SW: AAA+ proteins: have engine, will work. Nat Rev Mol Cell Biol. 2005, 6: 519-529.","journal-title":"Nat Rev Mol Cell Biol"},{"key":"568_CR7","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1016\/j.sbi.2007.09.012","volume":"17","author":"PA Tucker","year":"2007","unstructured":"Tucker PA, Sallai L: The AAA+ superfamily--a myriad of motions. Curr Opin Struct Biol. 2007, 17: 641-652. 10.1016\/j.sbi.2007.09.012.","journal-title":"Curr Opin Struct Biol"},{"key":"568_CR8","doi-asserted-by":"publisher","first-page":"1403","DOI":"10.1093\/hmg\/9.9.1403","volume":"9","author":"J Hewett","year":"2000","unstructured":"Hewett J, Gonzalez-Agosti C, Slater D, Ziefer P, Li S, Bergeron D, Jacoby DJ, Ozelius LJ, Ramesh V, Breakefield XO: Mutant torsinA, responsible for early-onset torsion dystonia, forms membrane inclusions in cultured neural cells. Hum Mol Genet. 2000, 9: 1403-1413. 10.1093\/hmg\/9.9.1403.","journal-title":"Hum Mol Genet"},{"key":"568_CR9","doi-asserted-by":"crossref","first-page":"27933","DOI":"10.1074\/jbc.M910025199","volume":"275","author":"K Kustedjo","year":"2000","unstructured":"Kustedjo K, Bracey MH, Cravatt BF: Torsin A and its torsion dystonia-associated mutant forms are lumenal glycoproteins that exhibit distinct subcellular localizations. J Biol Chem. 2000, 275: 27933-27939.","journal-title":"J Biol Chem"},{"key":"568_CR10","doi-asserted-by":"publisher","first-page":"847","DOI":"10.1073\/pnas.0304375101","volume":"101","author":"RE Goodchild","year":"2004","unstructured":"Goodchild RE, Dauer WT: Mislocalization to the nuclear envelope: An effect of the dystonia causing torsinA mutation. PNAS. 2004, 101: 847-852. 10.1073\/pnas.0304375101.","journal-title":"PNAS"},{"key":"568_CR11","doi-asserted-by":"publisher","first-page":"7612","DOI":"10.1073\/pnas.0308760101","volume":"101","author":"TV Naismith","year":"2004","unstructured":"Naismith TV, Heuser JE, Breakefield XO, Hanson PI: TorsinA in the nuclear envelope. Proc Natl Acad Sci USA. 2004, 101: 7612-7617. 10.1073\/pnas.0308760101.","journal-title":"Proc Natl Acad Sci USA"},{"key":"568_CR12","doi-asserted-by":"publisher","first-page":"855","DOI":"10.1083\/jcb.200411026","volume":"168","author":"RE Goodchild","year":"2005","unstructured":"Goodchild RE, Dauer WT: The AAA+ protein torsinA interacts with a conserved domain present in LAP1 and a novel ER protein. J Cell Biol. 2005, 168: 855-862. 10.1083\/jcb.200411026.","journal-title":"J Cell Biol"},{"key":"568_CR13","doi-asserted-by":"publisher","first-page":"27866","DOI":"10.1074\/jbc.M109.020164","volume":"284","author":"TV Naismith","year":"2009","unstructured":"Naismith TV, Dalal S, Hanson PI: Interaction of TorsinA with its major binding partners is impaired by the dystonia associated {Delta}GAG deletion. J Biol Chem. 2009, 284: 27866-27874. 10.1074\/jbc.M109.020164.","journal-title":"J Biol Chem"},{"key":"568_CR14","doi-asserted-by":"publisher","first-page":"9861","DOI":"10.1073\/pnas.0912877107","volume":"107","author":"CE Kim","year":"2010","unstructured":"Kim CE, Perez A, Perkins G, Ellisman MH, Dauer WT: A molecular mechanism underlying the neural-specific defect in torsinA mutant mice. Proc Natl Acad Sci USA. 2010, 107: 9861-9866. 10.1073\/pnas.0912877107.","journal-title":"Proc Natl Acad Sci USA"},{"key":"568_CR15","volume-title":"J Biol Chem","author":"L Zhu","year":"2010","unstructured":"Zhu L, Millen L, Mendoza JL, Thomas PJ: A unique redox-sensing sensor-II motif in TorsinA plays a critical role in nucleotide and partner binding. J Biol Chem. 2010"},{"key":"568_CR16","doi-asserted-by":"publisher","first-page":"3476","DOI":"10.1242\/jcs.029454","volume":"121","author":"FC Nery","year":"2008","unstructured":"Nery FC, Zeng J, Niland BP, Hewett J, Farley J, Irimia D, Li Y, Wiche G, Sonnenberg A, Breakefield XO: TorsinA binds the KASH domain of nesprins and participates in linkage between nuclear envelope and cytoskeleton. J Cell Sci. 2008, 121: 3476-3486. 10.1242\/jcs.029454.","journal-title":"J Cell Sci"},{"key":"568_CR17","doi-asserted-by":"publisher","first-page":"2593","DOI":"10.1523\/JNEUROSCI.4461-03.2004","volume":"24","author":"P Gonzalez-Alegre","year":"2004","unstructured":"Gonzalez-Alegre P, Paulson HL: Aberrant cellular behavior of mutant torsinA implicates nuclear envelope dysfunction in DYT1 dystonia. J Neurosci. 2004, 24: 2593-2601. 10.1523\/JNEUROSCI.4461-03.2004.","journal-title":"J Neurosci"},{"key":"568_CR18","doi-asserted-by":"publisher","first-page":"1741","DOI":"10.1007\/PL00000813","volume":"58","author":"L Holmer","year":"2001","unstructured":"Holmer L, Worman HJ: Inner nuclear membrane proteins: functions and targeting. Cell Mol Life Sci. 2001, 58: 1741-1747. 10.1007\/PL00000813.","journal-title":"Cell Mol Life Sci"},{"key":"568_CR19","doi-asserted-by":"publisher","first-page":"1373","DOI":"10.1042\/BST0361373","volume":"36","author":"N Zuleger","year":"2008","unstructured":"Zuleger N, Korfali N, Schirmer EC: Inner nuclear membrane protein transport is mediated by multiple mechanisms. Biochem Soc Trans. 2008, 36: 1373-1377. 10.1042\/BST0361373.","journal-title":"Biochem Soc Trans"},{"key":"568_CR20","doi-asserted-by":"publisher","first-page":"2661","DOI":"10.1091\/mbc.E09-01-0094","volume":"20","author":"AB Vander Heyden","year":"2009","unstructured":"Vander Heyden AB, Naismith TV, Snapp EL, Hodzic D, Hanson PI: LULL1 Retargets TorsinA to the Nuclear Envelope Revealing an Activity that Is Impaired by the DYT1 Dystonia Mutation. Mol Biol Cell. 2009, 20: 2661-2672. 10.1091\/mbc.E09-01-0094.","journal-title":"Mol Biol Cell"},{"key":"568_CR21","doi-asserted-by":"publisher","first-page":"888","DOI":"10.1093\/hmg\/ddp557","volume":"19","author":"M Jungwirth","year":"2010","unstructured":"Jungwirth M, Dear ML, Brown P, Holbrook K, Goodchild R: Relative tissue expression of homologous torsinB correlates with the neuronal specific importance of DYT1 dystonia-associated torsinA. Hum Mol Genet. 2010, 19: 888-900. 10.1093\/hmg\/ddp557.","journal-title":"Hum Mol Genet"},{"key":"568_CR22","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1083\/jcb.200509124","volume":"172","author":"M Crisp","year":"2006","unstructured":"Crisp M, Liu Q, Roux K, Rattner JB, Shanahan C, Burke B, Stahl PD, Hodzic D: Coupling of the nucleus and cytoplasm: role of the LINC complex. J Cell Biol. 2006, 172: 41-53. 10.1083\/jcb.200509124.","journal-title":"J Cell Biol"},{"key":"568_CR23","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/j.neuron.2009.08.018","volume":"64","author":"X Zhang","year":"2009","unstructured":"Zhang X, Lei K, Yuan X, Wu X, Zhuang Y, Xu T, Xu R, Han M: SUN1\/2 and Syne\/Nesprin-1\/2 complexes connect centrosome to the nucleus during neurogenesis and neuronal migration in mice. Neuron. 2009, 64: 173-187. 10.1016\/j.neuron.2009.08.018.","journal-title":"Neuron"},{"key":"568_CR24","doi-asserted-by":"publisher","first-page":"956","DOI":"10.1126\/science.1189072","volume":"329","author":"GW Luxton","year":"2010","unstructured":"Luxton GW, Gomes ER, Folker ES, Vintinner E, Gundersen GG: Linear arrays of nuclear envelope proteins harness retrograde actin flow for nuclear movement. Science. 2010, 329: 956-959. 10.1126\/science.1189072.","journal-title":"Science"},{"key":"568_CR25","volume-title":"Biochem J","author":"Z Liu","year":"2003","unstructured":"Liu Z, Zolkiewska A, Zolkiewski M: Characterization of human torsinA and its dystonia-associated mutant form. Biochem J. 2003, Pt:"},{"key":"568_CR26","doi-asserted-by":"publisher","first-page":"1147","DOI":"10.1038\/nsmb.1503","volume":"15","author":"A Martin","year":"2008","unstructured":"Martin A, Baker TA, Sauer RT: Pore loops of the AAA+ ClpX machine grip substrates to drive translocation and unfolding. Nat Struct Mol Biol. 2008, 15: 1147-1151. 10.1038\/nsmb.1503.","journal-title":"Nat Struct Mol Biol"},{"key":"568_CR27","doi-asserted-by":"publisher","first-page":"995","DOI":"10.1083\/jcb.200610072","volume":"176","author":"SR White","year":"2007","unstructured":"White SR, Evans KJ, Lary J, Cole JL, Lauring B: Recognition of C-terminal amino acids in tubulin by pore loops in Spastin is important for microtubule severing. J Cell Biol. 2007, 176: 995-1005. 10.1083\/jcb.200610072.","journal-title":"J Cell Biol"},{"key":"568_CR28","doi-asserted-by":"publisher","first-page":"13497","DOI":"10.1021\/bi801140y","volume":"47","author":"ME Farbman","year":"2008","unstructured":"Farbman ME, Gershenson A, Licht S: Role of a conserved pore residue in the formation of a prehydrolytic high substrate affinity state in the AAA+ chaperone ClpA. Biochemistry. 2008, 47: 13497-13505. 10.1021\/bi801140y.","journal-title":"Biochemistry"},{"key":"568_CR29","doi-asserted-by":"publisher","first-page":"2712","DOI":"10.1093\/hmg\/ddn173","volume":"17","author":"LM Giles","year":"2008","unstructured":"Giles LM, Chen J, Li L, Chin LS: Dystonia-associated mutations cause premature degradation of torsinA protein and cell-type-specific mislocalization to the nuclear envelope. Hum Mol Genet. 2008, 17: 2712-2722. 10.1093\/hmg\/ddn173.","journal-title":"Hum Mol Genet"},{"key":"568_CR30","volume-title":"J Cell Biol","author":"M Crisp","year":"2005","unstructured":"Crisp M, Liu Q, Roux K, Rattner JB, Shanahan C, Burke B, Stahl PD, Hodzic D: Coupling of the nucleus and cytoplasm: role of the LINC complex. J Cell Biol. 2005"},{"key":"568_CR31","doi-asserted-by":"publisher","first-page":"3738","DOI":"10.1128\/MCB.26.10.3738-3751.2006","volume":"26","author":"F Haque","year":"2006","unstructured":"Haque F, Lloyd DJ, Smallwood DT, Dent CL, Shanahan CM, Fry AM, Trembath RC, Shackleton S: SUN1 interacts with nuclear lamin A and cytoplasmic nesprins to provide a physical connection between the nuclear lamina and the cytoskeleton. Mol Cell Biol. 2006, 26: 3738-3751. 10.1128\/MCB.26.10.3738-3751.2006.","journal-title":"Mol Cell Biol"},{"key":"568_CR32","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1083\/jcb.200906068","volume":"186","author":"D Razafsky","year":"2009","unstructured":"Razafsky D, Hodzic D: Bringing KASH under the SUN: the many faces of nucleo-cytoskeletal connections. J Cell Biol. 2009, 186: 461-472. 10.1083\/jcb.200906068.","journal-title":"J Cell Biol"},{"key":"568_CR33","doi-asserted-by":"publisher","first-page":"2415","DOI":"10.1016\/j.bbamcr.2008.09.001","volume":"1783","author":"W Lu","year":"2008","unstructured":"Lu W, Gotzmann J, Sironi L, Jaeger VM, Schneider M, Luke Y, Uhlen M, Szigyarto CA, Brachner A, Ellenberg J, Foisner R, Noegel AA, Karakesisoglou I: Sun1 forms immobile macromolecular assemblies at the nuclear envelope. Biochim Biophys Acta. 2008, 1783: 2415-2426. 10.1016\/j.bbamcr.2008.09.001.","journal-title":"Biochim Biophys Acta"},{"key":"568_CR34","doi-asserted-by":"publisher","first-page":"785","DOI":"10.1083\/jcb.200704108","volume":"178","author":"Q Liu","year":"2007","unstructured":"Liu Q, Pante N, Misteli T, Elsagga M, Crisp M, Hodzic D, Burke B, Roux KJ: Functional association of Sun1 with nuclear pore complexes. J Cell Biol. 2007, 178: 785-798. 10.1083\/jcb.200704108.","journal-title":"J Cell Biol"},{"key":"568_CR35","doi-asserted-by":"publisher","first-page":"863","DOI":"10.1016\/j.devcel.2007.03.018","volume":"12","author":"X Ding","year":"2007","unstructured":"Ding X, Xu R, Yu J, Xu T, Zhuang Y, Han M: SUN1 is required for telomere attachment to nuclear envelope and gametogenesis in mice. Dev Cell. 2007, 12: 863-872. 10.1016\/j.devcel.2007.03.018.","journal-title":"Dev Cell"},{"key":"568_CR36","doi-asserted-by":"publisher","first-page":"10207","DOI":"10.1073\/pnas.0812037106","volume":"106","author":"K Lei","year":"2009","unstructured":"Lei K, Zhang X, Ding X, Guo X, Chen M, Zhu B, Xu T, Zhuang Y, Xu R, Han M: SUN1 and SUN2 play critical but partially redundant roles in anchoring nuclei in skeletal muscle cells in mice. Proc Natl Acad Sci USA. 2009, 106: 10207-10212. 10.1073\/pnas.0812037106.","journal-title":"Proc Natl Acad Sci USA"},{"key":"568_CR37","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1002\/pro.36","volume":"18","author":"M Nagy","year":"2009","unstructured":"Nagy M, Wu HC, Liu Z, Kedzierska-Mieszkowska S, Zolkiewski M: Walker-A threonine couples nucleotide occupancy with the chaperone activity of the AAA+ ATPase ClpB. Protein Sci. 2009, 18: 287-293. 10.1002\/pro.36.","journal-title":"Protein Sci"},{"key":"568_CR38","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1046\/j.1365-2443.2001.00447.x","volume":"6","author":"T Ogura","year":"2001","unstructured":"Ogura T, Wilkinson AJ: AAA+ superfamily ATPases: common structure--diverse function. Genes Cells. 2001, 6: 575-597. 10.1046\/j.1365-2443.2001.00447.x.","journal-title":"Genes Cells"},{"key":"568_CR39","doi-asserted-by":"publisher","first-page":"1241","DOI":"10.1093\/hmg\/ddm072","volume":"16","author":"E Fernandez-Vizarra","year":"2007","unstructured":"Fernandez-Vizarra E, Bugiani M, Goffrini P, Carrara F, Farina L, Procopio E, Donati A, Uziel G, Ferrero I, Zeviani M: Impaired complex III assembly associated with BCS1L gene mutations in isolated mitochondrial encephalopathy. Hum Mol Genet. 2007, 16: 1241-1252. 10.1093\/hmg\/ddm072.","journal-title":"Hum Mol Genet"},{"key":"568_CR40","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1016\/S0014-5793(02)03179-4","volume":"529","author":"DA Dougan","year":"2002","unstructured":"Dougan DA, Mogk A, Zeth K, Turgay K, Bukau B: AAA+ proteins and substrate recognition, it all depends on their partner in crime. FEBS Lett. 2002, 529: 6-10. 10.1016\/S0014-5793(02)03179-4.","journal-title":"FEBS Lett"},{"key":"568_CR41","volume-title":"Proc Natl Acad Sci USA","author":"AM Ulug","year":"2011","unstructured":"Ulug AM, Vo A, Argyelan M, Tanabe L, Schiffer WK, Dewey S, Dauer WT, Eidelberg D: Cerebellothalamocortical pathway abnormalities in torsinA DYT1 knock-in mice. Proc Natl Acad Sci USA. 2011"},{"key":"568_CR42","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1016\/j.neuroscience.2008.12.041","volume":"164","author":"M Carbon","year":"2009","unstructured":"Carbon M, Eidelberg D: Abnormal structure-function relationships in hereditary dystonia. Neuroscience. 2009, 164: 220-229. 10.1016\/j.neuroscience.2008.12.041.","journal-title":"Neuroscience"},{"key":"568_CR43","doi-asserted-by":"publisher","first-page":"8822","DOI":"10.1074\/jbc.270.15.8822","volume":"270","author":"L Martin","year":"1995","unstructured":"Martin L, Crimaudo C, Gerace L: cDNA cloning and characterization of lamina-associated polypeptide 1C (LAP1C), an integral protein of the inner nuclear membrane. J Biol Chem. 1995, 270: 8822-8828. 10.1074\/jbc.270.15.8822.","journal-title":"J Biol Chem"}],"container-title":["BMC Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2121-12-24.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T14:33:00Z","timestamp":1630506780000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcmolcellbiol.biomedcentral.com\/articles\/10.1186\/1471-2121-12-24"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,5,31]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,12]]}},"alternative-id":["568"],"URL":"https:\/\/doi.org\/10.1186\/1471-2121-12-24","relation":{},"ISSN":["1471-2121"],"issn-type":[{"value":"1471-2121","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,5,31]]},"assertion":[{"value":"4 January 2011","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 May 2011","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 May 2011","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"24"}}