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ASCs can differentiate into cells of multiple lineages, and as such are applicable to tissue engineering and regenerative medicine. Yet the question of whether ASC functionality is affected by the donor\u2019s body mass index (BMI) still exists.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      ASCs were isolated from patients having different BMIs (BMI-ASCs), within the ranges of 18.5-32.8. It was hypothesized that overweight BMI-ASCs would be more compromised in early adipogenic and osteogenic potential, and ability to form colonies\n                      <jats:italic>in vitro<\/jats:italic>\n                      . BMI was inversely correlated with ASC proliferation and colony forming potential as assessed by CyQUANT proliferation assay (fluorescence- based measurement of cellular DNA content), and colony forming assays. BMI was positively correlated with early time point (day 7) but not later time point (day 15) intracytoplasmic lipid accumulation as assessed by Oil-Red-O staining. Alizarin red staining and RT-PCR for alkaline phosphatase demonstrated that elevated BMI resulted in compromised ASC mineralization of extracellular matrix and decreased alkaline phosphatase mRNA expression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      These data demonstrate that elevated BMI resulted in reduced ASC proliferation, and potentially compromised osteogenic capacity\n                      <jats:italic>in vitro<\/jats:italic>\n                      ; thus BMI is an important criterion to consider in selecting ASC donors for clinical applications.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/1471-2121-14-34","type":"journal-article","created":{"date-parts":[[2013,8,7]],"date-time":"2013-08-07T12:14:00Z","timestamp":1375877640000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":65,"title":["Body mass index affects proliferation and osteogenic differentiation of human subcutaneous adipose tissue-derived stem cells"],"prefix":"10.1186","volume":"14","author":[{"given":"Trivia P","family":"Frazier","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey M","family":"Gimble","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica W","family":"Devay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugh A","family":"Tucker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernest S","family":"Chiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian G","family":"Rowan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,8,7]]},"reference":[{"key":"671_CR1","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1161\/01.CIR.93.1.54","volume":"93","author":"W Bao","year":"1996","unstructured":"Bao W, Srinivasan SR, Berenson GS: Persistent elevation of plasma insulin levels is associated with increased cardiovascular risk in children and young adults. 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