{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T21:20:16Z","timestamp":1793395216926,"version":"4.5.0"},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Cell Biol"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>In this study, we have investigated the viscoelastic behaviour of individual human adult bone marrow-derived mesenchymal stem cells (hMSCs) and the role of F-actin filaments in maintaining these properties, using micropipette aspiration technique together with a standard linear viscoelastic solid model.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Under a room temperature of 20\u00b0C, the instantaneous and equilibrium Young's modulus, <jats:italic>E<\/jats:italic>\n              <jats:sub>0<\/jats:sub> and <jats:italic>E<\/jats:italic>\n              <jats:sub>\u221e<\/jats:sub>, were found to be 886 \u00b1 289 Pa and 372 \u00b1 125 Pa, respectively, while the apparent viscosity, <jats:italic>\u03bc<\/jats:italic>, was 2710 \u00b1 1630 Pa\u00b7s. hMSCs treated with cytochalasin D up to 20 \u03bcM at 20\u00b0C registered significant drop of up to 84% in stiffness and increase of up to 255% in viscosity. At the physiological temperature of 37\u00b0C, <jats:italic>E<\/jats:italic>\n              <jats:sub>0<\/jats:sub> and <jats:italic>E<\/jats:italic>\n              <jats:sub>\u221e<\/jats:sub> have decreased by 42\u201366% whereas <jats:italic>\u03bc<\/jats:italic> has increased by 95%, compared to the control. Majority of the hMSCs behave as viscoelastic solid with a rapid initial increase in aspiration length and it gradually levels out with time. Three other types of non-typical viscoelastic behavior of hMSCs were also seen.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>hMSCs behave as viscoelastic solid. Its viscoelstic behaviour are dependent on the structural integrity of the F-actin filaments and temperature.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2121-9-40","type":"journal-article","created":{"date-parts":[[2008,7,22]],"date-time":"2008-07-22T06:15:14Z","timestamp":1216707314000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":82,"title":["Viscoelastic behaviour of human mesenchymal stem cells"],"prefix":"10.1186","volume":"9","author":[{"given":"Samuel CW","family":"Tan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen X","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kam W","family":"Leong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kin","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2008,7,22]]},"reference":[{"issue":"1","key":"318_CR1","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1002\/jcp.20959","volume":"211","author":"A Giordano","year":"2007","unstructured":"Giordano A, Galderisi U, Marino IR: From the laboratory bench to the patients bedside: An update on clinical trials with mesenchymal stem cells. 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