{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T03:34:28Z","timestamp":1777433668434,"version":"3.51.4"},"reference-count":19,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Dev Biol"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>The promyelocytic leukemia zinc finger gene <jats:italic>Plzf<\/jats:italic> (also called <jats:italic>Zbtb16, Zfp145<\/jats:italic> or <jats:italic>Green's luxoid<\/jats:italic>) belongs to the POZ\/zinc-finger family of transcription factors. It contains a BTB\/POZ domain that mediates epigenetic transcriptional repression. PLZF is essential for proper skeleton patterning and male germ cell renewal. Two alleles have been reported that display similar phenotypes: a targeted knock-out, and the spontaneous nonsense mutation <jats:italic>luxoid<\/jats:italic>.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We describe a new ENU induced missense allele of <jats:italic>Plzf<\/jats:italic> called seven toes (<jats:italic>Plzf<\/jats:italic>\n              <jats:sup>7<jats:italic>t<\/jats:italic>\n              <\/jats:sup>). Homozygous animals exhibit hindlimb and axial skeleton abnormalities. Whereas the skeletal abnormalities are similar to those of the other alleles, <jats:italic>Plzf<\/jats:italic>\n              <jats:sup>7<jats:italic>t<\/jats:italic>\n              <\/jats:sup>differs in that it does not cause spermatogonial depletion and infertility. Positional cloning revealed a point mutation changing the evolutionarily conserved amino acid Glu44 to Gly, possibly altering the BTB domain's activity.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>\n              <jats:italic>Plzf<\/jats:italic>\n              <jats:sup>7<jats:italic>t<\/jats:italic>\n              <\/jats:sup>is a separation-of-function allele that reveals differential requirements for domains of PLZF in different developmental milieus.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-213x-10-33","type":"journal-article","created":{"date-parts":[[2010,3,25]],"date-time":"2010-03-25T19:14:54Z","timestamp":1269544494000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["An allele separating skeletal patterning and spermatogonial renewal functions of PLZF"],"prefix":"10.1186","volume":"10","author":[{"given":"Yung-Hao","family":"Ching","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lawriston A","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John C","family":"Schimenti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2010,3,25]]},"reference":[{"key":"510_CR1","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1097\/00062752-200107000-00006","volume":"8","author":"JA Costoya","year":"2001","unstructured":"Costoya JA, Pandolfi PP: The role of promyelocytic leukemia zinc finger and promyelocytic leukemia in leukemogenesis and development. 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