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It is not known if NXT2 has any function in specific biological processes.<\/jats:p>\n                     <\/jats:sec><jats:sec>\n                        <jats:title>Results<\/jats:title>\n                        <jats:p>A zebrafish mutant exhibiting specific heart defects during embryogenesis was generated by animal cloning-mediated retroviral insertions. Molecular analysis indicated that the mutant phenotype was caused by a disruption of <jats:italic>NXT2<\/jats:italic>. Whole-mount RNA <jats:italic>in situ<\/jats:italic> hybridization showed that <jats:italic>NXT2<\/jats:italic> transcripts were clearly detectable in embryonic heart as well as other tissues. Further analysis revealed that expression level of one form of alternative splicing <jats:italic>NXT2<\/jats:italic> mRNA transcripts was significantly reduced, resulting in deficient myocardial cell differentiation and the malformation of cardiac valve at the atrioventricular boundary. The defects could be reproduced by morpholino anti-sense oligo knockdown of <jats:italic>NXT2<\/jats:italic>.<\/jats:p>\n                     <\/jats:sec><jats:sec>\n                        <jats:title>Conclusion<\/jats:title>\n                        <jats:p>NXT2 has a critical role in maintaining morphogenetic integrity of embryonic heart in vertebrate species.<\/jats:p>\n                     <\/jats:sec>","DOI":"10.1186\/1471-213x-5-7","type":"journal-article","created":{"date-parts":[[2005,3,26]],"date-time":"2005-03-26T07:16:10Z","timestamp":1111821370000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["NXT2 is required for embryonic heart development in zebrafish"],"prefix":"10.1186","volume":"5","author":[{"given":"Haigen","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parvana A","family":"Hartenstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jau-nian","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2005,3,24]]},"reference":[{"key":"58_CR1","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/S0074-7696(02)17012-4","volume":"217","author":"SM Steggerda","year":"2002","unstructured":"Steggerda SM, Paschal BM: Regulation of nuclear import and export by the GTPase Ran. 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