{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:14:19Z","timestamp":1791173659127,"version":"4.1.0"},"reference-count":57,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Dev Biol"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>The full-length mammalian homologs of <jats:italic>groucho<\/jats:italic>, Tle1, 2, 3, and 4, act as transcriptional corepressors and are recruited by transcription factors containing an eh1 or WRPW\/Y domain. Many transcription factors critical to pancreas development contain a Gro\/TLE interaction domain and several have been shown to require Gro\/TLE interactions for proper function during neuronal development. However, a detailed analysis of the expression patterns of the Gro\/TLE proteins in pancreas development has not been performed. Moreover, little is known about the ability of Gro\/TLE proteins to interact with transcription factors in the pancreas.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We describe the expression of Gro\/TLE family members, and of 34 different transcription factors that contain a Gro\/TLE interaction motif, in the pancreas utilizing nine SAGE libraries created from the developing and adult pancreas, as well as the <jats:italic>GenePaint<\/jats:italic> database. Next, we show the dynamic expression of <jats:italic>Tle1<\/jats:italic>, <jats:italic>2<\/jats:italic>, <jats:italic>3<\/jats:italic>, <jats:italic>4, 5<\/jats:italic> and <jats:italic>6<\/jats:italic> during pancreas development by qRT-PCR. To further define the cell-type specificity of the expression of these proteins we use immunofluorescence to co-localize them with Pdx1 at embryonic day 12.5 (E12.5), Ngn3 at E14.5, Pdx1, Nkx2-2, Insulin, Glucagon, Pancreatic polypeptide and Somatostatin at E18.5, as well as Insulin and Glucagon in the adult. We then show that Tle2 can interact with Nkx2-2, Hes1, Arx, and Nkx6-1 which are all critical factors in pancreas development. Finally, we demonstrate that Tle2 modulates the repressive abilities of Arx in a \u03b2-cell line.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Although Tle1, 2, 3, and 4 show overlapping expression in pancreatic progenitors and in the adult islet, the expression of these factors is restricted to different cell types during endocrine cell maturation. Of note, Tle2 and Tle3 are co-expressed with Gro\/TLE interaction domain containing transcription factors that are essential for endocrine pancreas development. We further demonstrate that Tle2 can interact with several of these factors and that Tle2 modulate Arx's repressive activity. Taken together our studies suggest that Gro\/TLE proteins play a role in the repression of target genes during endocrine cell specification.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-213x-8-81","type":"journal-article","created":{"date-parts":[[2008,9,9]],"date-time":"2008-09-09T06:14:06Z","timestamp":1220940846000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Expression of Groucho\/TLE proteins during pancreas development"],"prefix":"10.1186","volume":"8","author":[{"given":"Brad G","family":"Hoffman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bogard","family":"Zavaglia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike","family":"Beach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheryl D","family":"Helgason","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2008,9,8]]},"reference":[{"issue":"16","key":"365_CR1","doi-asserted-by":"publisher","first-page":"16284","DOI":"10.1074\/jbc.M500491200","volume":"280","author":"Q Wei","year":"2005","unstructured":"Wei Q, Miskimins WK, Miskimins R: Stage-specific expression of myelin basic protein in oligodendrocytes involves NKX2.2-mediated repression that is relieved by the Sp1 transcription factor. J Biol Chem. 2005, 280 (16): 16284-16294. 10.1074\/jbc.M500491200.","journal-title":"J Biol Chem"},{"key":"365_CR2","doi-asserted-by":"publisher","first-page":"1413","DOI":"10.1016\/S0960-9822(01)00441-9","volume":"11","author":"T Sun","year":"2001","unstructured":"Sun T, Echelard Y, Lu R, Yuk DI, Kaing S, Stiles CD, Rowitch DH: Olig bHLH proteins interact with homeodomain proteins to regulate cell fate acquisition in progenitors of the ventral neural tube. Curr Biol. 2001, 11: 1413-1420. 10.1016\/S0960-9822(01)00441-9.","journal-title":"Curr Biol"},{"key":"365_CR3","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1242\/dev.02763","volume":"134","author":"MJ Doyle","year":"2007","unstructured":"Doyle MJ, Loomis ZL, Sussel L: Nkx2.2-repressor activity is sufficient to specify {alpha}-cells and a small number of {beta}-cells in the pancreatic islet. Development. 2007, 134: 515-523. 10.1242\/dev.02763.","journal-title":"Development"},{"key":"365_CR4","doi-asserted-by":"publisher","first-page":"2969","DOI":"10.1242\/dev.01870","volume":"132","author":"P Collombat","year":"2005","unstructured":"Collombat P, Hecksher-Sorensen J, Broccoli V, Krull J, Ponte I, Mundiger T, Smith J, Gruss P, Serup P, Mansouri A: The simultaneous loss of Arx and Pax4 genes promotes a somatostatin-producing cell fate specification at the expense of the alpha- and beta-cell lineages in the mouse endocrine pancreas. Development. 2005, 132: 2969-2980. 10.1242\/dev.01870.","journal-title":"Development"},{"key":"365_CR5","doi-asserted-by":"publisher","first-page":"1363","DOI":"10.1210\/me.2004-0006","volume":"18","author":"T Iype","year":"2004","unstructured":"Iype T, Taylor DG, Ziesmann SM, Garmey JC, Watada H, Mirmira RG: The transcriptional repressor Nkx6.1 also functions as a deoxyribonucleic acid context-dependent transcriptional activator during pancreatic beta-cell differentiation: evidence for feedback activation of the nkx6.1 gene by Nkx6.1. Mol Endocrinol. 2004, 18: 1363-1375. 10.1210\/me.2004-0006.","journal-title":"Mol Endocrinol"},{"key":"365_CR6","doi-asserted-by":"publisher","first-page":"14743","DOI":"10.1074\/jbc.275.19.14743","volume":"275","author":"RG Mirmira","year":"2000","unstructured":"Mirmira RG, Watada H, German MS: Beta-cell differentiation factor Nkx6.1 contains distinct DNA binding interference and transcriptional repression domains. J Biol Chem. 2000, 275: 14743-14751. 10.1074\/jbc.275.19.14743.","journal-title":"J Biol Chem"},{"issue":"Suppl","key":"365_CR7","doi-asserted-by":"publisher","first-page":"1183","DOI":"10.1038\/nn750","volume":"4","author":"SK Lee","year":"2001","unstructured":"Lee SK, Pfaff SL: Transcriptional networks regulating neuronal identity in the developing spinal cord. Nat Neurosci. 2001, 4 (Suppl): 1183-1191. 10.1038\/nn750.","journal-title":"Nat Neurosci"},{"key":"365_CR8","doi-asserted-by":"publisher","first-page":"861","DOI":"10.1016\/S0092-8674(01)00283-5","volume":"104","author":"J Muhr","year":"2001","unstructured":"Muhr J, Andersson E, Persson M, Jessell TM, Ericson J: Groucho-mediated transcriptional repression establishes progenitor cell pattern and neuronal fate in the ventral neural tube. Cell. 2001, 104: 861-873. 10.1016\/S0092-8674(01)00283-5.","journal-title":"Cell"},{"key":"365_CR9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0378-1119(00)00161-X","volume":"249","author":"G Chen","year":"2000","unstructured":"Chen G, Courey AJ: Groucho\/TLE family proteins and transcriptional repression. Gene. 2000, 249: 1-16. 10.1016\/S0378-1119(00)00161-X.","journal-title":"Gene"},{"key":"365_CR10","doi-asserted-by":"publisher","first-page":"3072","DOI":"10.1101\/gad.11.22.3072","volume":"11","author":"G Jimenez","year":"1997","unstructured":"Jimenez G, Paroush Z, Ish-Horowicz D: Groucho acts as a corepressor for a subset of negative regulators, including Hairy and Engrailed. Genes Dev. 1997, 11: 3072-3082. 10.1101\/gad.11.22.3072.","journal-title":"Genes Dev"},{"key":"365_CR11","doi-asserted-by":"publisher","first-page":"670","DOI":"10.1002\/jcb.20476","volume":"95","author":"M Gasperowicz","year":"2005","unstructured":"Gasperowicz M, Otto F: Mammalian Groucho homologs: redundancy or specificity?. J Cell Biochem. 2005, 95: 670-687. 10.1002\/jcb.20476.","journal-title":"J Cell Biochem"},{"key":"365_CR12","doi-asserted-by":"publisher","first-page":"26604","DOI":"10.1074\/jbc.272.42.26604","volume":"272","author":"A Palaparti","year":"1997","unstructured":"Palaparti A, Baratz A, Stifani S: The Groucho\/transducin-like enhancer of split transcriptional repressors interact with the genetically defined amino-terminal silencing domain of histone H3. J Biol Chem. 1997, 272: 26604-26610. 10.1074\/jbc.272.42.26604.","journal-title":"J Biol Chem"},{"key":"365_CR13","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1016\/0925-4773(95)00452-1","volume":"53","author":"G Dehni","year":"1995","unstructured":"Dehni G, Liu Y, Husain J, Stifani S: TLE expression correlates with mouse embryonic segmentation, neurogenesis, and epithelial determination. Mech Dev. 1995, 53: 369-381. 10.1016\/0925-4773(95)00452-1.","journal-title":"Mech Dev"},{"key":"365_CR14","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1046\/j.1440-169X.1998.00003.x","volume":"40","author":"J Yao","year":"1998","unstructured":"Yao J, Liu Y, Husain J, Lo R, Palaparti A, Henderson J, Stifani S: Combinatorial expression patterns of individual TLE proteins during cell determination and differentiation suggest non-redundant functions for mammalian homologs of Drosophila Groucho. Dev Growth Differ. 1998, 40: 133-146. 10.1046\/j.1440-169X.1998.00003.x.","journal-title":"Dev Growth Differ"},{"key":"365_CR15","first-page":"687","volume":"45","author":"T Allen","year":"1999","unstructured":"Allen T, Lobe CG: A comparison of Notch, Hes and Grg expression during murine embryonic and post-natal development. Cell Mol Biol (Noisy-le-grand). 1999, 45: 687-708.","journal-title":"Cell Mol Biol (Noisy-le-grand)"},{"key":"365_CR16","doi-asserted-by":"publisher","first-page":"2152","DOI":"10.1210\/me.2003-0225","volume":"17","author":"ML Brinkmeier","year":"2003","unstructured":"Brinkmeier ML, Potok MA, Cha KB, Gridley T, Stifani S, Meeldijk J, Clevers H, Camper SA: TCF and Groucho-related genes influence pituitary growth and development. Mol Endocrinol. 2003, 17: 2152-2161. 10.1210\/me.2003-0225.","journal-title":"Mol Endocrinol"},{"key":"365_CR17","doi-asserted-by":"publisher","first-page":"5522","DOI":"10.1093\/emboj\/cdg536","volume":"22","author":"Y Cai","year":"2003","unstructured":"Cai Y, Brophy PD, Levitan I, Stifani S, Dressler GR: Groucho suppresses Pax2 transactivation by inhibition of JNK-mediated phosphorylation. Embo J. 2003, 22: 5522-5529. 10.1093\/emboj\/cdg536.","journal-title":"Embo J"},{"key":"365_CR18","doi-asserted-by":"publisher","first-page":"65","DOI":"10.3109\/08830180109056723","volume":"20","author":"SL Nutt","year":"2001","unstructured":"Nutt SL, Eberhard D, Horcher M, Rolink AG, Busslinger M: Pax5 determines the identity of B cells from the beginning to the end of B-lymphopoiesis. Int Rev Immunol. 2001, 20: 65-82.","journal-title":"Int Rev Immunol"},{"key":"365_CR19","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1002\/dvdy.10089","volume":"224","author":"WF Wang","year":"2002","unstructured":"Wang WF, Wang YG, Reginato AM, Plotkina S, Gridley T, Olsen BR: Growth defect in Grg5 null mice is associated with reduced Ihh signaling in growth plates. Dev Dyn. 2002, 224: 79-89. 10.1002\/dvdy.10089.","journal-title":"Dev Dyn"},{"key":"365_CR20","doi-asserted-by":"publisher","first-page":"760","DOI":"10.1002\/dvdy.20398","volume":"233","author":"B Bajoghli","year":"2005","unstructured":"Bajoghli B, Aghaallaei N, Czerny T: Groucho corepressor proteins regulate otic vesicle outgrowth. Dev Dyn. 2005, 233: 760-771. 10.1002\/dvdy.20398.","journal-title":"Dev Dyn"},{"key":"365_CR21","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1242\/dev.00185","volume":"130","author":"J Lopez-Rios","year":"2003","unstructured":"Lopez-Rios J, Tessmar K, Loosli F, Wittbrodt J, Bovolenta P: Six3 and Six6 activity is modulated by members of the groucho family. Development. 2003, 130: 185-195. 10.1242\/dev.00185.","journal-title":"Development"},{"key":"365_CR22","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1006\/dbio.2001.0185","volume":"232","author":"M Kobayashi","year":"2001","unstructured":"Kobayashi M, Nishikawa K, Suzuki T, Yamamoto M: The homeobox protein Six3 interacts with the Groucho corepressor and acts as a transcriptional repressor in eye and forebrain formation. Dev Biol. 2001, 232: 315-326. 10.1006\/dbio.2001.0185.","journal-title":"Dev Biol"},{"key":"365_CR23","doi-asserted-by":"publisher","first-page":"1410","DOI":"10.1093\/nar\/29.7.1410","volume":"29","author":"H Brantjes","year":"2001","unstructured":"Brantjes H, Roose J, Wetering van De M, Clevers H: All Tcf HMG box transcription factors interact with Groucho-related co-repressors. Nucleic Acids Res. 2001, 29: 1410-1419. 10.1093\/nar\/29.7.1410.","journal-title":"Nucleic Acids Res"},{"key":"365_CR24","doi-asserted-by":"publisher","first-page":"18418","DOI":"10.1074\/jbc.M910211199","volume":"275","author":"JC Wang","year":"2000","unstructured":"Wang JC, Waltner-Law M, Yamada K, Osawa H, Stifani S, Granner DK: Transducin-like enhancer of split proteins, the human homologs of Drosophila groucho, interact with hepatic nuclear factor 3beta. J Biol Chem. 2000, 275: 18418-18423. 10.1074\/jbc.M910211199.","journal-title":"J Biol Chem"},{"key":"365_CR25","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1128\/MCB.22.2.389-399.2002","volume":"22","author":"HN Nuthall","year":"2002","unstructured":"Nuthall HN, Husain J, McLarren KW, Stifani S: Role for Hes1-induced phosphorylation in Groucho-mediated transcriptional repression. Mol Cell Biol. 2002, 22: 389-399. 10.1128\/MCB.22.2.389-399.2002.","journal-title":"Mol Cell Biol"},{"key":"365_CR26","doi-asserted-by":"publisher","first-page":"1578","DOI":"10.1074\/jbc.M007629200","volume":"276","author":"KW McLarren","year":"2001","unstructured":"McLarren KW, Theriault FM, Stifani S: Association with the nuclear matrix and interaction with Groucho and RUNX proteins regulate the transcription repression activity of the basic helix loop helix factor Hes1. J Biol Chem. 2001, 276: 1578-1584. 10.1074\/jbc.M007629200.","journal-title":"J Biol Chem"},{"key":"365_CR27","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1046\/j.1432-1327.1998.2580339.x","volume":"258","author":"D Grbavec","year":"1998","unstructured":"Grbavec D, Lo R, Liu Y, Stifani S: Transducin-like Enhancer of split 2, a mammalian homologue of Drosophila Groucho, acts as a transcriptional repressor, interacts with Hairy\/Enhancer of split proteins, and is expressed during neuronal development. Eur J Biochem. 1998, 258: 339-349. 10.1046\/j.1432-1327.1998.2580339.x.","journal-title":"Eur J Biochem"},{"key":"365_CR28","doi-asserted-by":"publisher","first-page":"1962","DOI":"10.1128\/MCB.21.6.1962-1972.2001","volume":"21","author":"J Yao","year":"2001","unstructured":"Yao J, Lai E, Stifani S: The winged-helix protein brain factor 1 interacts with groucho and hes proteins to repress transcription. Mol Cell Biol. 2001, 21: 1962-1972. 10.1128\/MCB.21.6.1962-1972.2001.","journal-title":"Mol Cell Biol"},{"key":"365_CR29","doi-asserted-by":"publisher","first-page":"2134","DOI":"10.1101\/gad.820400","volume":"14","author":"M Sander","year":"2000","unstructured":"Sander M, Paydar S, Ericson J, Briscoe J, Berber E, German M, Jessell TM, Rubenstein JL: Ventral neural patterning by Nkx homeobox genes: Nkx6.1 controls somatic motor neuron and ventral interneuron fates. Genes Dev. 2000, 14: 2134-2139. 10.1101\/gad.820400.","journal-title":"Genes Dev"},{"key":"365_CR30","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1016\/S0896-6273(01)00412-3","volume":"31","author":"A Vallstedt","year":"2001","unstructured":"Vallstedt A, Muhr J, Pattyn A, Pierani A, Mendelsohn M, Sander M, Jessell TM, Ericson J: Different levels of repressor activity assign redundant and specific roles to Nkx6 genes in motor neuron and interneuron specification. Neuron. 2001, 31: 743-755. 10.1016\/S0896-6273(01)00412-3.","journal-title":"Neuron"},{"key":"365_CR31","doi-asserted-by":"publisher","first-page":"18485","DOI":"10.1073\/pnas.0509455102","volume":"102","author":"AS Siddiqui","year":"2005","unstructured":"Siddiqui AS, Khattra J, Delaney AD, Zhao Y, Astell C, Asano J, Babakaiff R, Barber S, Beland J, Bohacec S, Brown-John M, Chand S, Charest D, Charters AM, Cullum R, Dhalla N, Featherstone R, Gerhard DS, Hoffman B, Holt RA, Hou J, Kuo BY, Lee LL, Lee S, Leung D, Ma K, Matsuo C, Mayo M, McDonald H, Prabhu AL, et al: A mouse atlas of gene expression: large-scale digital gene-expression profiles from precisely defined developing C57BL\/6J mouse tissues and cells. Proc Natl Acad Sci USA. 2005, 102: 18485-18490. 10.1073\/pnas.0509455102.","journal-title":"Proc Natl Acad Sci USA"},{"key":"365_CR32","doi-asserted-by":"publisher","first-page":"R99","DOI":"10.1186\/gb-2008-9-6-r99","volume":"9","author":"BG Hoffman","year":"2008","unstructured":"Hoffman BG, Zavaglia B, Witzsche J, Ruiz de Algara T, Beach M, Hoodless PA, Jones SJ, Marra MA, Helgason CD: Identification of transcripts with enriched expression in the developing and adult pancreas. Genome Biol. 2008, 9: R99-10.1186\/gb-2008-9-6-r99.","journal-title":"Genome Biol"},{"key":"365_CR33","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1016\/S0925-4773(00)00317-8","volume":"94","author":"E Lammert","year":"2000","unstructured":"Lammert E, Brown J, Melton DA: Notch gene expression during pancreatic organogenesis. Mech Dev. 2000, 94: 199-203. 10.1016\/S0925-4773(00)00317-8.","journal-title":"Mech Dev"},{"key":"365_CR34","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1038\/12669","volume":"23","author":"H Li","year":"1999","unstructured":"Li H, Arber S, Jessell TM, Edlund H: Selective agenesis of the dorsal pancreas in mice lacking homeobox gene Hlxb9. Nat Genet. 1999, 23: 67-70.","journal-title":"Nat Genet"},{"key":"365_CR35","doi-asserted-by":"crossref","first-page":"5533","DOI":"10.1242\/dev.127.24.5533","volume":"127","author":"M Sander","year":"2000","unstructured":"Sander M, Sussel L, Conners J, Scheel D, Kalamaras J, Dela Cruz F, Schwitzgebel V, Hayes-Jordan A, German M: Homeobox gene Nkx6.1 lies downstream of Nkx2.2 in the major pathway of beta-cell formation in the pancreas. Development. 2000, 127: 5533-5540.","journal-title":"Development"},{"key":"365_CR36","doi-asserted-by":"publisher","first-page":"2491","DOI":"10.1242\/dev.002691","volume":"134","author":"SB Nelson","year":"2007","unstructured":"Nelson SB, Schaffer AE, Sander M: The transcription factors Nkx6.1 and Nkx6.2 possess equivalent activities in promoting beta-cell fate specification in Pdx1+ pancreatic progenitor cells. Development. 2007, 134: 2491-2500. 10.1242\/dev.002691.","journal-title":"Development"},{"key":"365_CR37","doi-asserted-by":"publisher","first-page":"1203","DOI":"10.1007\/s001250100628","volume":"44","author":"C McKinnon","year":"2001","unstructured":"McKinnon C, Docherty K: Pancreatic duodenal homeobox-1, PDX-1, a major reulator of beta cell identity and function. Diabetologia. 2001, 44: 1203-1214. 10.1007\/s001250100628.","journal-title":"Diabetologia"},{"key":"365_CR38","doi-asserted-by":"crossref","first-page":"2447","DOI":"10.1242\/dev.129.10.2447","volume":"129","author":"G Gu","year":"2002","unstructured":"Gu G, Dubauskaite J, Melton D: Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors. Development. 2002, 129: 2447-2457.","journal-title":"Development"},{"key":"365_CR39","doi-asserted-by":"publisher","first-page":"18749","DOI":"10.1074\/jbc.271.31.18749","volume":"271","author":"J Jensen","year":"1996","unstructured":"Jensen J, Serup P, Karlsen C, Nielsen TF, Madsen OD: mRNA profiling of rat islet tumors reveals nkx 6.1 as a beta-cell-specific homeodomain transcription factor. J Biol Chem. 1996, 271: 18749-18758. 10.1074\/jbc.271.31.18749.","journal-title":"J Biol Chem"},{"key":"365_CR40","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1038\/71657","volume":"24","author":"J Jensen","year":"2000","unstructured":"Jensen J, Pedersen EE, Galante P, Hald J, Heller RS, Ishibashi M, Kageyama R, Guillemot F, Serup P, Madsen OD: Control of endodermal endocrine development by Hes-1. Nat Genet. 2000, 24: 36-44. 10.1038\/72814.","journal-title":"Nat Genet"},{"key":"365_CR41","doi-asserted-by":"publisher","first-page":"163","DOI":"10.2337\/diabetes.49.2.163","volume":"49","author":"J Jensen","year":"2000","unstructured":"Jensen J, Heller RS, Funder-Nielsen T, Pedersen EE, Lindsell C, Weinmaster G, Madsen OD, Serup P: Independent development of pancreatic alpha- and beta-cells from neurogenin3-expressing precursors: a role for the notch pathway in repression of premature differentiation. Diabetes. 2000, 49: 163-176. 10.2337\/diabetes.49.2.163.","journal-title":"Diabetes"},{"key":"365_CR42","doi-asserted-by":"publisher","first-page":"877","DOI":"10.1038\/23716","volume":"400","author":"A Apelqvist","year":"1999","unstructured":"Apelqvist A, Li H, Sommer L, Beatus P, Anderson D, Honjo T, Hrabe de Angelis M, Lendahl U, Edlund H: Notch signalling controls pancreatic cell differentiation. Nature. 1999, 400: 877-881. 10.1038\/23716.","journal-title":"Nature"},{"key":"365_CR43","doi-asserted-by":"publisher","first-page":"14920","DOI":"10.1073\/pnas.2436557100","volume":"100","author":"LC Murtaugh","year":"2003","unstructured":"Murtaugh LC, Stanger BZ, Kwan KM, Melton DA: Notch signaling controls multiple steps of pancreatic differentiation. Proc Natl Acad Sci USA. 2003, 100: 14920-14925. 10.1073\/pnas.2436557100.","journal-title":"Proc Natl Acad Sci USA"},{"key":"365_CR44","doi-asserted-by":"publisher","first-page":"961","DOI":"10.1172\/JCI29115","volume":"117","author":"P Collombat","year":"2007","unstructured":"Collombat P, Hecksher-Sorensen J, Krull J, Berger J, Riedel D, Herrera PL, Serup P, Mansouri A: Embryonic endocrine pancreas and mature beta cells acquire alpha and PP cell phenotypes upon Arx misexpression. J Clin Invest. 2007, 117: 961-970. 10.1172\/JCI29115.","journal-title":"J Clin Invest"},{"key":"365_CR45","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1016\/j.neuroscience.2007.01.038","volume":"146","author":"O McKenzie","year":"2007","unstructured":"McKenzie O, Ponte I, Mangelsdorf M, Finnis M, Colasante G, Shoubridge C, Stifani S, Gecz J, Broccoli V: Aristaless-related homeobox gene, the gene responsible for West syndrome and related disorders, is a Groucho\/transducin-like enhancer of split dependent transcriptional repressor. Neuroscience. 2007, 146: 236-247. 10.1016\/j.neuroscience.2007.01.038.","journal-title":"Neuroscience"},{"key":"365_CR46","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/S0925-4773(00)00278-1","volume":"93","author":"J Yao","year":"2000","unstructured":"Yao J, Liu Y, Lo R, Tretjakoff I, Peterson A, Stifani S: Disrupted development of the cerebral hemispheres in transgenic mice expressing the mammalian Groucho homologue transducin-like-enhancer of split 1 in postmitotic neurons. Mech Dev. 2000, 93: 105-115. 10.1016\/S0925-4773(00)00278-1.","journal-title":"Mech Dev"},{"key":"365_CR47","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1002\/dvdy.10460","volume":"229","author":"J Jensen","year":"2004","unstructured":"Jensen J: Gene regulatory factors in pancreatic development. Dev Dyn. 2004, 229: 176-200. 10.1002\/dvdy.10460.","journal-title":"Dev Dyn"},{"key":"365_CR48","doi-asserted-by":"publisher","first-page":"51049","DOI":"10.1074\/jbc.M111660200","volume":"277","author":"HN Nuthall","year":"2002","unstructured":"Nuthall HN, Joachim K, Palaparti A, Stifani S: A role for cell cycle-regulated phosphorylation in Groucho-mediated transcriptional repression. J Biol Chem. 2002, 277: 51049-51057. 10.1074\/jbc.M111660200.","journal-title":"J Biol Chem"},{"key":"365_CR49","doi-asserted-by":"publisher","first-page":"10916","DOI":"10.1128\/MCB.25.24.10916-10929.2005","volume":"25","author":"N Marcal","year":"2005","unstructured":"Marcal N, Patel H, Dong Z, Belanger-Jasmin S, Hoffman B, Helgason CD, Dang J, Stifani S: Antagonistic effects of Grg6 and Groucho\/TLE on the transcription repression activity of brain factor 1\/FoxG1 and cortical neuron differentiation. Mol Cell Biol. 2005, 25: 10916-10929. 10.1128\/MCB.25.24.10916-10929.2005.","journal-title":"Mol Cell Biol"},{"key":"365_CR50","doi-asserted-by":"publisher","first-page":"2591","DOI":"10.1101\/gad.269003","volume":"17","author":"P Collombat","year":"2003","unstructured":"Collombat P, Mansouri A, Hecksher-Sorensen J, Serup P, Krull J, Gradwohl G, Gruss P: Opposing actions of Arx and Pax4 in endocrine pancreas development. Genes Dev. 2003, 17: 2591-2603. 10.1101\/gad.269003.","journal-title":"Genes Dev"},{"key":"365_CR51","doi-asserted-by":"publisher","first-page":"4149","DOI":"10.1242\/dev.00641","volume":"130","author":"A Pattyn","year":"2003","unstructured":"Pattyn A, Vallstedt A, Dias JM, Sander M, Ericson J: Complementary roles for Nkx6 and Nkx2 class proteins in the establishment of motoneuron identity in the hindbrain. Development. 2003, 130: 4149-4159. 10.1242\/dev.00641.","journal-title":"Development"},{"key":"365_CR52","doi-asserted-by":"publisher","first-page":"3139","DOI":"10.1242\/dev.01875","volume":"132","author":"KD Henseleit","year":"2005","unstructured":"Henseleit KD, Nelson SB, Kuhlbrodt K, Hennings JC, Ericson J, Sander M: NKX6 transcription factor activity is required for alpha- and beta-cell development in the pancreas. Development. 2005, 132: 3139-3149. 10.1242\/dev.01875.","journal-title":"Development"},{"key":"365_CR53","doi-asserted-by":"publisher","first-page":"1808","DOI":"10.1210\/en.2004-1175","volume":"146","author":"L Marzban","year":"2005","unstructured":"Marzban L, Soukhatcheva G, Verchere CB: Role of carboxypeptidase E in processing of pro-islet amyloid polypeptide in {beta}-cells. Endocrinology. 2005, 146: 1808-1817. 10.1210\/en.2004-1175.","journal-title":"Endocrinology"},{"key":"365_CR54","doi-asserted-by":"publisher","first-page":"R6","DOI":"10.1186\/gb-2007-8-1-r6","volume":"8","author":"N Robertson","year":"2007","unstructured":"Robertson N, Oveisi-Fordorei M, Zuyderduyn SD, Varhol RJ, Fjell C, Marra M, Jones S, Siddiqui A: DiscoverySpace: an interactive data analysis application. Genome Biol. 2007, 8: R6-10.1186\/gb-2007-8-1-r6.","journal-title":"Genome Biol"},{"key":"365_CR55","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1101\/gr.8.3.186","volume":"8","author":"B Ewing","year":"1998","unstructured":"Ewing B, Green P: Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Res. 1998, 8: 186-194.","journal-title":"Genome Res"},{"key":"365_CR56","doi-asserted-by":"publisher","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","volume":"29","author":"MW Pfaffl","year":"2001","unstructured":"Pfaffl MW: A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001, 29: e45-10.1093\/nar\/29.9.e45.","journal-title":"Nucleic Acids Res"},{"key":"365_CR57","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/j.devcel.2007.06.001","volume":"13","author":"Q Zhou","year":"2007","unstructured":"Zhou Q, Law AC, Rajagopal J, Anderson WJ, Gray PA, Melton DA: A multipotent progenitor domain guides pancreatic organogenesis. Dev Cell. 2007, 13: 103-114. 10.1016\/j.devcel.2007.06.001.","journal-title":"Dev Cell"}],"container-title":["BMC Developmental Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-213X-8-81.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T09:53:25Z","timestamp":1630490005000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcdevbiol.biomedcentral.com\/articles\/10.1186\/1471-213X-8-81"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,9,8]]},"references-count":57,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,12]]}},"alternative-id":["365"],"URL":"https:\/\/doi.org\/10.1186\/1471-213x-8-81","relation":{},"ISSN":["1471-213X"],"issn-type":[{"value":"1471-213X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,9,8]]},"assertion":[{"value":"28 April 2008","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 September 2008","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 September 2008","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"81"}}