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Land snakes target various prey species including insects (arthropods), lizards (reptiles), frogs and toads (amphibians), birds (aves), and rodents (mammals), whereas sea snakes target a single vertebrate class (fishes) and often specialize on specific types of fish. It is therefore interesting to examine the evolution of toxins in sea snake venoms compared to that of land snakes.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Here we describe the expression of toxin genes in the venom glands of two sea snakes, <jats:italic>Lapemis curtus<\/jats:italic> (Spine-bellied Sea Snake) and <jats:italic>Acalyptophis peronii<\/jats:italic> (Horned Sea Snake), two members of a large adaptive radiation which occupy very different ecological niches. We constructed cDNA libraries from their venom glands and sequenced 214 and 192 clones, respectively. Our data show that despite their explosive evolutionary radiation, there is very little variability in the three-finger toxin (3FTx) as well as the phospholipase A<jats:sub>2<\/jats:sub> (PLA<jats:sub>2<\/jats:sub>) enzymes, the two main constituents of <jats:italic>Lapemis curtus<\/jats:italic> and <jats:italic>Acalyptophis peronii<\/jats:italic> venom. To understand the evolutionary trends among land snakes, sea snakes and sea kraits, pairwise genetic distances (intraspecific and interspecific) of 3FTx and PLA<jats:sub>2<\/jats:sub> sequences were calculated. Results show that these proteins appear to be highly conserved in sea snakes in contrast to land snakes or sea kraits, despite their extremely divergent and adaptive ecological radiation.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Based on these results, we suggest that streamlining in habitat and diet in sea snakes has possibly kept their toxin genes conserved, suggesting the idea that prey composition and diet breadth may contribute to the diversity and evolution of venom components.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2148-7-175","type":"journal-article","created":{"date-parts":[[2007,9,27]],"date-time":"2007-09-27T18:13:40Z","timestamp":1190916820000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Expression pattern of three-finger toxin and phospholipase A2 genes in the venom glands of two sea snakes, Lapemis curtus and Acalyptophis peronii: comparison of evolution of these toxins in land snakes, sea kraits and sea snakes"],"prefix":"10.1186","volume":"7","author":[{"given":"Susanta","family":"Pahari","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Bickford","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryan G","family":"Fry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R Manjunatha","family":"Kini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2007,9,27]]},"reference":[{"key":"466_CR1","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1016\/0041-0101(82)90019-8","volume":"20","author":"JP Chippaux","year":"1982","unstructured":"Chippaux JP, Boche J, Courtois B: Electrophoretic patterns of the venoms from a litter of Bitis gabonica snakes. 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