{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T21:46:34Z","timestamp":1780695994449,"version":"3.54.1"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2011,5,10]],"date-time":"2011-05-10T00:00:00Z","timestamp":1304985600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2011,5,10]],"date-time":"2011-05-10T00:00:00Z","timestamp":1304985600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Genomics"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Our previous studies revealed that a new disease form of streptococcal toxic shock syndrome (STSS) is associated with specific <jats:italic>Streptococcus suis<\/jats:italic> serotype 2 (SS2) strains. To achieve a better understanding of the pathogenicity and evolution of SS2 at the whole-genome level, comparative genomic analysis of 18 SS2 strains, selected on the basis of virulence and geographic origin, was performed using NimbleGen tiling arrays.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Our results demonstrate that SS2 isolates have highly divergent genomes. The 89K pathogenicity island (PAI), which has been previously recognized as unique to the Chinese epidemic strains causing STSS, was partially included in some other virulent and avirulent strains. The ABC-type transport systems, encoded by 89K, were hypothesized to greatly contribute to the catastrophic features of STSS. Moreover, we identified many polymorphisms in genes encoding candidate or known virulence factors, such as PlcR, lipase, sortases, the pilus-associated proteins, and the response regulator RevS and CtsR. On the basis of analysis of regions of differences (RDs) across the entire genome for the 18 selected SS2 strains, a model of microevolution for these strains is proposed, which provides clues into <jats:italic>Streptococcus<\/jats:italic> pathogenicity and evolution.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Our deep comparative genomic analysis of the 89K PAI present in the genome of SS2 strains revealed details into how some virulent strains acquired genes that may contribute to STSS, which may lead to better environmental monitoring of epidemic SS2 strains.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2164-12-219","type":"journal-article","created":{"date-parts":[[2011,5,11]],"date-time":"2011-05-11T06:22:08Z","timestamp":1305094928000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Probing genomic diversity and evolution of Streptococcus suis serotype 2 by NimbleGen tiling arrays"],"prefix":"10.1186","volume":"12","author":[{"given":"Zuowei","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changjun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruifen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongqiang","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruifu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuihua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George F","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoli","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2011,5,10]]},"reference":[{"issue":"6","key":"10150_CR1","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1023\/A:1005870317757","volume":"21","author":"JJ Staats","year":"1997","unstructured":"Staats JJ, Feder I, Okwumabua O, Chengappa MM: Streptococcus suis: past and present. Vet Res Commun. 1997, 21 (6): 381-407. 10.1023\/A:1005870317757.","journal-title":"Vet Res Commun"},{"issue":"3","key":"10150_CR2","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1016\/j.tim.2009.12.003","volume":"18","author":"Y Feng","year":"2010","unstructured":"Feng Y, Zhang H, Ma Y, Gao GF: Uncovering newly emerging variants of Streptococcus suis, an important zoonotic agent. Trends Microbiol. 2010, 18 (3): 124-131. 10.1016\/j.tim.2009.12.003.","journal-title":"Trends Microbiol"},{"issue":"1530","key":"10150_CR3","doi-asserted-by":"publisher","first-page":"2725","DOI":"10.1098\/rstb.2009.0093","volume":"364","author":"Y Ma","year":"2009","unstructured":"Ma Y, Feng Y, Liu D, Gao GF: Avian influenza virus, Streptococcus suis serotype 2, severe acute respiratory syndrome-coronavirus and beyond: molecular epidemiology, ecology and the situation in China. Philos Trans R Soc Lond B Biol Sci. 2009, 364 (1530): 2725-2737. 10.1098\/rstb.2009.0093.","journal-title":"Philos Trans R Soc Lond B Biol Sci"},{"key":"10150_CR4","doi-asserted-by":"publisher","first-page":"e151","DOI":"10.1371\/journal.pmed.0030151","volume":"3","author":"J Tang","year":"2006","unstructured":"Tang J, Wang C, Feng Y, Yang W, Song H, Chen Z, Yu H, Pan X, Zhou X, Wang H, et al: Streptococcal toxic shock syndrome caused by Streptococcus suis serotype 2. PLoS Med. 2006, 3: e151-10.1371\/journal.pmed.0030151.","journal-title":"PLoS Med"},{"issue":"6","key":"10150_CR5","doi-asserted-by":"publisher","first-page":"1670","DOI":"10.1111\/j.1365-2958.2011.07553.x","volume":"79","author":"M Li","year":"2011","unstructured":"Li M, Shen X, Yan J, Han H, Zheng B, Liu D, Cheng H, Zhao Y, Rao X, Wang C, et al: GI-type T4SS-mediated horizontal transfer of the 89 K pathogenicity island in epidemic Streptococcus suis serotype 2. Mol Microbiol. 2011, 79 (6): 1670-1683. 10.1111\/j.1365-2958.2011.07553.x.","journal-title":"Mol Microbiol"},{"key":"10150_CR6","doi-asserted-by":"publisher","first-page":"e315","DOI":"10.1371\/journal.pone.0000315","volume":"2","author":"C Chen","year":"2007","unstructured":"Chen C, Tang J, Dong W, Wang C, Feng Y, Wang J, Zheng F, Pan X, Liu D, Li M, et al: A Glimpse of Streptococcal Toxic Shock Syndrome from Comparative Genomics of S. suis 2 Chinese Isolates. PLoS ONE. 2007, 2: e315-10.1371\/journal.pone.0000315.","journal-title":"PLoS ONE"},{"issue":"7","key":"10150_CR7","doi-asserted-by":"publisher","first-page":"e6072","DOI":"10.1371\/journal.pone.0006072","volume":"4","author":"MT Holden","year":"2009","unstructured":"Holden MT, Hauser H, Sanders M, Ngo TH, Cherevach I, Cronin A, Goodhead I, Mungall K, Quail MA, Price C, et al: Rapid evolution of virulence and drug resistance in the emerging zoonotic pathogen Streptococcus suis. PLoS One. 2009, 4 (7): e6072-10.1371\/journal.pone.0006072.","journal-title":"PLoS One"},{"key":"10150_CR8","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1186\/1471-2164-9-413","volume":"9","author":"AS Leung","year":"2008","unstructured":"Leung AS, Tran V, Wu Z, Yu X, Alexander DC, Gao GF, Zhu B, Liu J: Novel genome polymorphisms in BCG vaccine strains and impact on efficacy. BMC Genomics. 2008, 9: 413-10.1186\/1471-2164-9-413.","journal-title":"BMC Genomics"},{"issue":"3","key":"10150_CR9","doi-asserted-by":"publisher","first-page":"398","DOI":"10.1101\/gr.2141004","volume":"14","author":"CW Wong","year":"2004","unstructured":"Wong CW, Albert TJ, Vega VB, Norton JE, Cutler DJ, Richmond TA, Stanton LW, Liu ET, Miller LD: Tracking the evolution of the SARS coronavirus using high-throughput, high-density resequencing arrays. Genome Res. 2004, 14 (3): 398-405. 10.1101\/gr.2141004.","journal-title":"Genome Res"},{"issue":"5","key":"10150_CR10","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1051\/vetres:2000133","volume":"31","author":"F Berthelot-Herault","year":"2000","unstructured":"Berthelot-Herault F, Morvan H, Keribin AM, Gottschalk M, Kobisch M: Production of muraminidase-released protein (MRP), extracellular factor (EF) and suilysin by field isolates of Streptococcus suis capsular types 2, 1\/2, 9, 7 and 3 isolated from swine in France. Vet Res. 2000, 31 (5): 473-479. 10.1051\/vetres:2000133.","journal-title":"Vet Res"},{"issue":"8","key":"10150_CR11","doi-asserted-by":"publisher","first-page":"3318","DOI":"10.1128\/IAI.61.8.3318-3326.1993","volume":"61","author":"HE Smith","year":"1993","unstructured":"Smith HE, Reek FH, Vecht U, Gielkens AL, Smits MA: Repeats in an extracellular protein of weakly pathogenic strains of Streptococcus suis type 2 are absent in pathogenic strains. Infect Immun. 1993, 61 (8): 3318-3326.","journal-title":"Infect Immun"},{"issue":"9","key":"10150_CR12","doi-asserted-by":"publisher","first-page":"3156","DOI":"10.1128\/IAI.59.9.3156-3162.1991","volume":"59","author":"U Vecht","year":"1991","unstructured":"Vecht U, Wisselink HJ, Jellema ML, Smith HE: Identification of two proteins associated with virulence of Streptococcus suis type 2. Infect Immun. 1991, 59 (9): 3156-3162.","journal-title":"Infect Immun"},{"issue":"4","key":"10150_CR13","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1016\/S0882-4010(02)90526-7","volume":"33","author":"A de Greeff","year":"2002","unstructured":"de Greeff A, Buys H, van Alphen L, Smith HE: Response regulator important in pathogenesis of Streptococcus suis serotype 2. Microb Pathog. 2002, 33 (4): 185-192.","journal-title":"Microb Pathog"},{"issue":"5","key":"10150_CR14","doi-asserted-by":"publisher","first-page":"e2080","DOI":"10.1371\/journal.pone.0002080","volume":"3","author":"M Li","year":"2008","unstructured":"Li M, Wang C, Feng Y, Pan X, Cheng G, Wang J, Ge J, Zheng F, Cao M, Dong Y, et al: SalK\/SalR, a two-component signal transduction system, is essential for full virulence of highly invasive Streptococcus suis serotype 2. PLoS One. 2008, 3 (5): e2080-10.1371\/journal.pone.0002080.","journal-title":"PLoS One"},{"issue":"5","key":"10150_CR15","doi-asserted-by":"publisher","first-page":"e187","DOI":"10.1371\/journal.pmed.0030187","volume":"3","author":"S Sriskandan","year":"2006","unstructured":"Sriskandan S, Slater JD: Invasive disease and toxic shock due to zoonotic Streptococcus suis: an emerging infection in the East?. PLoS Med. 2006, 3 (5): e187-10.1371\/journal.pmed.0030187.","journal-title":"PLoS Med"},{"issue":"2","key":"10150_CR16","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1128\/MMBR.00031-07","volume":"72","author":"AL Davidson","year":"2008","unstructured":"Davidson AL, Dassa E, Orelle C, Chen J: Structure, function, and evolution of bacterial ATP-binding cassette systems. Microbiol Mol Biol Rev. 2008, 72 (2): 317-364. 10.1128\/MMBR.00031-07.","journal-title":"Microbiol Mol Biol Rev"},{"issue":"47","key":"10150_CR17","doi-asserted-by":"publisher","first-page":"18490","DOI":"10.1073\/pnas.0704501104","volume":"104","author":"N Declerck","year":"2007","unstructured":"Declerck N, Bouillaut L, Chaix D, Rugani N, Slamti L, Hoh F, Lereclus D, Arold ST: Structure of PlcR: Insights into virulence regulation and evolution of quorum sensing in Gram-positive bacteria. Proc Natl Acad Sci USA. 2007, 104 (47): 18490-18495. 10.1073\/pnas.0704501104.","journal-title":"Proc Natl Acad Sci USA"},{"issue":"7","key":"10150_CR18","doi-asserted-by":"publisher","first-page":"e2793","DOI":"10.1371\/journal.pone.0002793","volume":"3","author":"M Gohar","year":"2008","unstructured":"Gohar M, Faegri K, Perchat S, Ravnum S, Okstad OA, Gominet M, Kolsto AB, Lereclus D: The PlcR virulence regulon of Bacillus cereus. PLoS One. 2008, 3 (7): e2793-10.1371\/journal.pone.0002793.","journal-title":"PLoS One"},{"issue":"6","key":"10150_CR19","doi-asserted-by":"publisher","first-page":"782","DOI":"10.1016\/j.biotechadv.2009.06.001","volume":"27","author":"F Hasan","year":"2009","unstructured":"Hasan F, Shah AA, Hameed A: Methods for detection and characterization of lipases: A comprehensive review. Biotechnol Adv. 2009, 27 (6): 782-798. 10.1016\/j.biotechadv.2009.06.001.","journal-title":"Biotechnol Adv"},{"issue":"2","key":"10150_CR20","first-page":"109","volume":"95","author":"J Rollof","year":"1987","unstructured":"Rollof J, Hedstrom SA, Nilsson-Ehle P: Lipolytic activity of Staphylococcus aureus strains from disseminated and localized infections. Acta Pathol Microbiol Immunol Scand B. 1987, 95 (2): 109-113.","journal-title":"Acta Pathol Microbiol Immunol Scand B"},{"key":"10150_CR21","doi-asserted-by":"publisher","first-page":"1419","DOI":"10.1099\/00221287-146-6-1419","volume":"146","author":"CM Longshaw","year":"2000","unstructured":"Longshaw CM, Farrell AM, Wright JD, Holland KT: Identification of a second lipase gene, gehD, in Staphylococcus epidermidis: comparison of sequence with those of other staphylococcal lipases. Microbiology. 2000, 146: 1419-1427.","journal-title":"Microbiology"},{"issue":"22","key":"10150_CR22","doi-asserted-by":"publisher","first-page":"6977","DOI":"10.1128\/JB.181.22.6977-6986.1999","volume":"181","author":"S Wilhelm","year":"1999","unstructured":"Wilhelm S, Tommassen J, Jaeger KE: A novel lipolytic enzyme located in the outer membrane of Pseudomonas aeruginosa. J Bacteriol. 1999, 181 (22): 6977-6986.","journal-title":"J Bacteriol"},{"issue":"1","key":"10150_CR23","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1007\/s00203-008-0425-z","volume":"191","author":"C Wang","year":"2009","unstructured":"Wang C, Li M, Feng Y, Zheng F, Dong Y, Pan X, Cheng G, Dong R, Hu D, Feng X, et al: The involvement of sortase A in high virulence of STSS-causing Streptococcus suis serotype 2. Arch Microbiol. 2009, 191 (1): 23-33. 10.1007\/s00203-008-0425-z.","journal-title":"Arch Microbiol"},{"issue":"4","key":"10150_CR24","doi-asserted-by":"publisher","first-page":"971","DOI":"10.1128\/jb.184.4.971-982.2002","volume":"184","author":"M Osaki","year":"2002","unstructured":"Osaki M, Takamatsu D, Shimoji Y, Sekizaki T: Characterization of Streptococcus suis genes encoding proteins homologous to sortase of gram-positive bacteria. J Bacteriol. 2002, 184 (4): 971-982. 10.1128\/jb.184.4.971-982.2002.","journal-title":"J Bacteriol"},{"issue":"3-4","key":"10150_CR25","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1016\/j.vetmic.2007.08.032","volume":"127","author":"G Vanier","year":"2008","unstructured":"Vanier G, Sekizaki T, Dominguez-Punaro MC, Esgleas M, Osaki M, Takamatsu D, Segura M, Gottschalk M: Disruption of srtA gene in Streptococcus suis results in decreased interactions with endothelial cells and extracellular matrix proteins. Vet Microbiol. 2008, 127 (3-4): 417-424. 10.1016\/j.vetmic.2007.08.032.","journal-title":"Vet Microbiol"},{"issue":"1","key":"10150_CR26","doi-asserted-by":"publisher","first-page":"e8426","DOI":"10.1371\/journal.pone.0008426","volume":"5","author":"Nahuel Fittipaldi","year":"2010","unstructured":"Fittipaldi N, Takamatsu D, de la Cruz Dominguez-Punaro M, Lecours MP, Montpetit D, Osaki M, Sekizaki T, Gottschalk M: Mutations in the gene encoding the ancillary pilin subunit of the Streptococcus suis srtF cluster result in pili formed by the major subunit only. PLoS One. 5 (1): e8426-","journal-title":"PLoS ONE"},{"issue":"3","key":"10150_CR27","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1111\/j.1365-2958.2008.06396.x","volume":"70","author":"S Falker","year":"2008","unstructured":"Falker S, Nelson AL, Morfeldt E, Jonas K, Hultenby K, Ries J, Melefors O, Normark S, Henriques-Normark B: Sortase-mediated assembly and surface topology of adhesive pneumococcal pili. Mol Microbiol. 2008, 70 (3): 595-607. 10.1111\/j.1365-2958.2008.06396.x.","journal-title":"Mol Microbiol"},{"issue":"43","key":"10150_CR28","doi-asserted-by":"publisher","first-page":"15641","DOI":"10.1073\/pnas.0507808102","volume":"102","author":"M Mora","year":"2005","unstructured":"Mora M, Bensi G, Capo S, Falugi F, Zingaretti C, Manetti AG, Maggi T, Taddei AR, Grandi G, Telford JL: Group A Streptococcus produce pilus-like structures containing protective antigens and Lancefield T antigens. Proc Natl Acad Sci USA. 2005, 102 (43): 15641-15646. 10.1073\/pnas.0507808102.","journal-title":"Proc Natl Acad Sci USA"},{"issue":"1","key":"10150_CR29","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1111\/j.1365-2958.2006.05225.x","volume":"61","author":"R Rosini","year":"2006","unstructured":"Rosini R, Rinaudo CD, Soriani M, Lauer P, Mora M, Maione D, Taddei A, Santi I, Ghezzo C, Brettoni C, et al: Identification of novel genomic islands coding for antigenic pilus-like structures in Streptococcus agalactiae. Mol Microbiol. 2006, 61 (1): 126-141. 10.1111\/j.1365-2958.2006.05225.x.","journal-title":"Mol Microbiol"},{"issue":"5","key":"10150_CR30","first-page":"1389","volume":"45","author":"DL Hava","year":"2002","unstructured":"Hava DL, Camilli A: Large-scale identification of serotype 4 Streptococcus pneumoniae virulence factors. Mol Microbiol. 2002, 45 (5): 1389-1406.","journal-title":"Mol Microbiol"},{"issue":"7","key":"10150_CR31","doi-asserted-by":"publisher","first-page":"1822","DOI":"10.1111\/j.1462-5822.2007.00918.x","volume":"9","author":"EL Abbot","year":"2007","unstructured":"Abbot EL, Smith WD, Siou GP, Chiriboga C, Smith RJ, Wilson JA, Hirst BH, Kehoe MA: Pili mediate specific adhesion of Streptococcus pyogenes to human tonsil and skin. Cell Microbiol. 2007, 9 (7): 1822-1833. 10.1111\/j.1462-5822.2007.00918.x.","journal-title":"Cell Microbiol"},{"issue":"2","key":"10150_CR32","doi-asserted-by":"publisher","first-page":"1059","DOI":"10.1128\/IAI.01400-06","volume":"75","author":"C Gianfaldoni","year":"2007","unstructured":"Gianfaldoni C, Censini S, Hilleringmann M, Moschioni M, Facciotti C, Pansegrau W, Masignani V, Covacci A, Rappuoli R, Barocchi MA, et al: Streptococcus pneumoniae pilus subunits protect mice against lethal challenge. Infect Immun. 2007, 75 (2): 1059-1062. 10.1128\/IAI.01400-06.","journal-title":"Infect Immun"},{"key":"10150_CR33","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1007\/978-0-387-78885-2_15","volume":"631","author":"S Dubrac","year":"2008","unstructured":"Dubrac S, Msadek T: Tearing down the wall: peptidoglycan metabolism and the WalK\/WalR (YycG\/YycF) essential two-component system. Adv Exp Med Biol. 2008, 631: 214-228. 10.1007\/978-0-387-78885-2_15.","journal-title":"Adv Exp Med Biol"},{"issue":"2","key":"10150_CR34","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1046\/j.1365-2958.2000.02124.x","volume":"38","author":"I Derre","year":"2000","unstructured":"Derre I, Rapoport G, Msadek T: The CtsR regulator of stress response is active as a dimer and specifically degraded in vivo at 37 degrees C. Mol Microbiol. 2000, 38 (2): 335-347. 10.1046\/j.1365-2958.2000.02124.x.","journal-title":"Mol Microbiol"},{"issue":"5","key":"10150_CR35","doi-asserted-by":"publisher","first-page":"1975","DOI":"10.1128\/IAI.63.5.1975-1979.1995","volume":"63","author":"S Quessy","year":"1995","unstructured":"Quessy S, Dubreuil JD, Caya M, Higgins R: Discrimination of virulent and avirulent Streptococcus suis capsular type 2 isolates from different geographical origins. Infect Immun. 1995, 63 (5): 1975-1979.","journal-title":"Infect Immun"},{"issue":"3","key":"10150_CR36","first-page":"236","volume":"69","author":"F Berthelot-Herault","year":"2005","unstructured":"Berthelot-Herault F, Gottschalk M, Morvan H, Kobisch M: Dilemma of virulence of Streptococcus suis: Canadian isolate 89-1591 characterized as a virulent strain using a standardized experimental model in pigs. Can J Vet Res. 2005, 69 (3): 236-240.","journal-title":"Can J Vet Res"},{"issue":"5","key":"10150_CR37","doi-asserted-by":"publisher","first-page":"R71","DOI":"10.1186\/gb-2007-8-5-r71","volume":"8","author":"T Lefebure","year":"2007","unstructured":"Lefebure T, Stanhope MJ: Evolution of the core and pan-genome of Streptococcus: positive selection, recombination, and genome composition. Genome Biol. 2007, 8 (5): R71-10.1186\/gb-2007-8-5-r71.","journal-title":"Genome Biol"},{"issue":"11","key":"10150_CR38","doi-asserted-by":"publisher","first-page":"1749","DOI":"10.1101\/gr.362402","volume":"12","author":"EF Nuwaysir","year":"2002","unstructured":"Nuwaysir EF, Huang W, Albert TJ, Singh J, Nuwaysir K, Pitas A, Richmond T, Gorski T, Berg JP, Ballin J, et al: Gene expression analysis using oligonucleotide arrays produced by maskless photolithography. Genome Res. 2002, 12 (11): 1749-1755. 10.1101\/gr.362402.","journal-title":"Genome Res"},{"issue":"7","key":"10150_CR39","doi-asserted-by":"publisher","first-page":"e35","DOI":"10.1093\/nar\/gng035","volume":"31","author":"TJ Albert","year":"2003","unstructured":"Albert TJ, Norton J, Ott M, Richmond T, Nuwaysir K, Nuwaysir EF, Stengele KP, Green RD: Light-directed 5'-- > 3' synthesis of complex oligonucleotide microarrays. Nucleic Acids Res. 2003, 31 (7): e35-10.1093\/nar\/gng035.","journal-title":"Nucleic Acids Res"},{"key":"10150_CR40","first-page":"69","volume-title":"Proc IEEE Comput Syst Bioinform Conf","author":"M Molla","year":"2004","unstructured":"Molla M, Shavlik J, Richmond T, Smith S: A self-tuning method for one-chip SNP identification. Proc IEEE Comput Syst Bioinform Conf. 2004, 69-79."}],"container-title":["BMC Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2164-12-219.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/1471-2164-12-219\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2164-12-219.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T14:06:06Z","timestamp":1630505166000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcgenomics.biomedcentral.com\/articles\/10.1186\/1471-2164-12-219"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,5,10]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,12]]}},"alternative-id":["10150"],"URL":"https:\/\/doi.org\/10.1186\/1471-2164-12-219","relation":{},"ISSN":["1471-2164"],"issn-type":[{"value":"1471-2164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,5,10]]},"assertion":[{"value":"13 December 2010","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 May 2011","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 May 2011","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"219"}}