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Studies of these changes are expected to reveal molecular targets for novel vector control and pathogen transmission-blocking strategies. The mosquito<jats:italic>Aedes aegypti<\/jats:italic>(Diptera, Culicidae), a vector of Dengue viruses, Yellow Fever Virus (YFV) and Chikungunya virus (CV), is the subject of this study to look at genome-wide changes in gene expression following a blood meal.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Transcriptional changes that follow a blood meal in<jats:italic>Ae. aegypti<\/jats:italic>females were explored using RNA-seq technology. Over 30% of more than 18,000 investigated transcripts accumulate differentially in mosquitoes at five hours after a blood meal when compared to those fed only on sugar. Forty transcripts accumulate only in blood-fed mosquitoes. The list of regulated transcripts correlates with an enhancement of digestive activity and a suppression of environmental stimuli perception and innate immunity. The alignment of more than 65 million high-quality short reads to the<jats:italic>Ae. aegypti<\/jats:italic>reference genome permitted the refinement of the current annotation of transcript boundaries, as well as the discovery of novel transcripts, exons and splicing variants.<jats:italic>Cis<\/jats:italic>-regulatory elements (CRE) and<jats:italic>cis<\/jats:italic>-regulatory modules (CRM) enriched significantly at the 5'end flanking sequences of blood meal-regulated genes were identified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study provides the first global view of the changes in transcript accumulation elicited by a blood meal in<jats:italic>Ae. aegypti<\/jats:italic>females. This information permitted the identification of classes of potentially co-regulated genes and a description of biochemical and physiological events that occur immediately after blood feeding. The data presented here serve as a basis for novel vector control and pathogen transmission-blocking strategies including those in which the vectors are modified genetically to express anti-pathogen effector molecules.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1471-2164-12-82","type":"journal-article","created":{"date-parts":[[2011,2,8]],"date-time":"2011-02-08T14:52:33Z","timestamp":1297176753000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":140,"title":["RNA-seq analyses of blood-induced changes in gene expression in the mosquito vector species, Aedes aegypti"],"prefix":"10.1186","volume":"12","author":[{"given":"Mariangela","family":"Bonizzoni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W Augustine","family":"Dunn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corey L","family":"Campbell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken E","family":"Olson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle T","family":"Dimon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osvaldo","family":"Marinotti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony A","family":"James","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2011,1,28]]},"reference":[{"key":"3213_CR1","doi-asserted-by":"crossref","first-page":"583","DOI":"10.20506\/rst.28.2.1904","volume":"28","author":"DJ Gubler","year":"2009","unstructured":"Gubler DJ: Vector-borne diseases. 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