{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T03:06:16Z","timestamp":1791342376972,"version":"4.3.1"},"reference-count":73,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Genomics"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>\n              <jats:italic>Mycobacterium ulcerans<\/jats:italic> is an unusual bacterial pathogen with elusive origins. While closely related to the aquatic dwelling <jats:italic>M. marinum<\/jats:italic>, <jats:italic>M. ulcerans<\/jats:italic> has evolved the ability to produce the immunosuppressive polyketide toxin mycolactone and cause the neglected tropical disease Buruli ulcer. Other mycolactone-producing mycobacteria (MPM) have been identified in fish and frogs and given distinct species designations (<jats:italic>M. pseudoshottsii, M. shinshuense<\/jats:italic>, <jats:italic>M. liflandii<\/jats:italic> and <jats:italic>M. marinum<\/jats:italic>), however the evolution of <jats:italic>M. ulcerans<\/jats:italic> and its relationship to other MPM has not been defined. Here we report the comparative analysis of whole genome sequences from 30 MPM and five <jats:italic>M. marinum<\/jats:italic>.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>A high-resolution phylogeny based on genome-wide single nucleotide polymorphisms (SNPs) showed that <jats:italic>M. ulcerans<\/jats:italic> and all other MPM represent a single clonal group that evolved from a common <jats:italic>M. marinum<\/jats:italic> progenitor. The emergence of the MPM was driven by the acquisition of the pMUM plasmid encoding genes for the biosynthesis of mycolactones. This change was accompanied by the loss of at least 185 genes, with a significant overrepresentation of genes associated with cell wall functions. Cell wall associated genes also showed evidence of substantial adaptive selection, suggesting cell wall remodeling has been critical for the survival of MPM. Fine-grain analysis of the MPM complex revealed at least three distinct lineages, one of which comprised a highly clonal group, responsible for Buruli ulcer in Africa and Australia. This indicates relatively recent transfer of <jats:italic>M. ulcerans<\/jats:italic> between these continents, which represent the vast majority of the global Buruli ulcer burden. Our data provide SNPs and gene sequences that can differentiate <jats:italic>M. ulcerans<\/jats:italic> lineages, suitable for use in the diagnosis and surveillance of Buruli ulcer.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>\n              <jats:italic>M. ulcerans<\/jats:italic> and all mycolactone-producing mycobacteria are specialized variants of a common <jats:italic>Mycobacterium marinum<\/jats:italic> progenitor that have adapted to live in restricted environments. Examination of genes lost or retained and now under selective pressure suggests these environments might be aerobic, and extracellular, where slow growth, production of an immune suppressor, cell wall remodeling, loss or modification of cell wall antigens, and biofilm-forming ability provide a survival advantage. These insights will guide our efforts to find the elusive reservoir(s) of <jats:italic>M. ulcerans<\/jats:italic> and to understand transmission of Buruli ulcer.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2164-13-258","type":"journal-article","created":{"date-parts":[[2012,6,29]],"date-time":"2012-06-29T16:35:52Z","timestamp":1340987752000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":163,"title":["On the origin of Mycobacterium ulcerans, the causative agent of Buruli ulcer"],"prefix":"10.1186","volume":"13","author":[{"given":"Kenneth D","family":"Doig","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn E","family":"Holt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet AM","family":"Fyfe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline J","family":"Lavender","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Eddyani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Portaels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorothy","family":"Yeboah-Manu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerd","family":"Pluschke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torsten","family":"Seemann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy P","family":"Stinear","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,6,19]]},"reference":[{"issue":"2","key":"4131_CR1","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1101\/gr.5942807","volume":"17","author":"TP Stinear","year":"2007","unstructured":"Stinear TP, Seemann T, Pidot S, Frigui W, Reysset G, Garnier T, Meurice G, Simon D, Bouchier C, Ma L: Reductive evolution and niche adaptation inferred from the genome ofMycobacterium ulcerans, the causative agent of Buruli ulcer. 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